全文获取类型
收费全文 | 21159篇 |
免费 | 1894篇 |
国内免费 | 304篇 |
专业分类
电工技术 | 29篇 |
综合类 | 675篇 |
化学工业 | 6608篇 |
金属工艺 | 152篇 |
机械仪表 | 315篇 |
建筑科学 | 142篇 |
矿业工程 | 34篇 |
能源动力 | 42篇 |
轻工业 | 13333篇 |
水利工程 | 29篇 |
石油天然气 | 180篇 |
武器工业 | 1篇 |
无线电 | 258篇 |
一般工业技术 | 909篇 |
冶金工业 | 137篇 |
原子能技术 | 53篇 |
自动化技术 | 460篇 |
出版年
2024年 | 218篇 |
2023年 | 503篇 |
2022年 | 1391篇 |
2021年 | 1484篇 |
2020年 | 842篇 |
2019年 | 896篇 |
2018年 | 761篇 |
2017年 | 856篇 |
2016年 | 761篇 |
2015年 | 884篇 |
2014年 | 1018篇 |
2013年 | 1212篇 |
2012年 | 1345篇 |
2011年 | 1351篇 |
2010年 | 980篇 |
2009年 | 896篇 |
2008年 | 812篇 |
2007年 | 1062篇 |
2006年 | 956篇 |
2005年 | 803篇 |
2004年 | 688篇 |
2003年 | 525篇 |
2002年 | 479篇 |
2001年 | 327篇 |
2000年 | 263篇 |
1999年 | 297篇 |
1998年 | 204篇 |
1997年 | 155篇 |
1996年 | 191篇 |
1995年 | 182篇 |
1994年 | 192篇 |
1993年 | 152篇 |
1992年 | 145篇 |
1991年 | 94篇 |
1990年 | 76篇 |
1989年 | 85篇 |
1988年 | 53篇 |
1987年 | 57篇 |
1986年 | 38篇 |
1985年 | 35篇 |
1984年 | 29篇 |
1983年 | 6篇 |
1982年 | 6篇 |
1981年 | 4篇 |
1980年 | 30篇 |
1979年 | 4篇 |
1978年 | 2篇 |
1976年 | 3篇 |
1973年 | 1篇 |
1951年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
81.
Masuhiro Tsukada Hiroshi Katoh Donna Wilson Bong‐Seob Shin Takayuki Arai Ritsuko Murakami Giuliano Freddi 《应用聚合物科学杂志》2002,86(5):1181-1188
The work presented here discusses a new technique for preparing silk fibers and films with persistent antimicrobial activity through use of metallic dyestuffs during the fiber dyeing process. The length of the silk fibers investigated contracted when the fibers were immersed in concentrated neutral salt solutions, such as calcium or potassium nitrate, at elevated temperature levels. The birefringence and molecular orientation of the silk fibroin molecules became less ordered by the action of the neutral salt solutions, resulting in increased dyestuff absorption. Subsequently, contracted silk fibers were dyed with metallic dyestuffs containing Cr or Cu for the purpose of obtaining silk fibers with antimicrobial activity. Silk fibers dyed with metallic dyestuffs showed significant antimicrobial activity against the plant pathogen Cornebacterium and the human pathogen Coli bacillus. Tensile strength of the silk fibers after the salt shrinking and dyeing processes did not show a significant change, whereas the elongation at break was increased slightly. The techniques described here for preparing significantly active antimicrobial silk fibers are effective and economic ways of providing new materials for industrial and biomedical applications. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 86: 1181–1188, 2002 相似文献
82.
作者应用前 S_1、前 S_2和 HBsAg/a 单克隆抗体,用免疫斑点法(Immuno-spot)检测同一批的乙肝表面抗原分别经加热灭活和三步化学灭活后的前 S_1和前 S_2蛋白保留情况,比较了两种工艺对前 S_1和前 S_2蛋白的影响。结果表明;加热灭活可保留前 S_1和前 S_2蛋白,三步化学灭活使前 S_1和前 S_2蛋白丢失,从抗原组成上看,加热灭活后的抗原更接近自然抗原。首次报告了含有前 S_1蛋白的乙肝疫苗,并对前 S_1和前 S_2蛋白在乙肝血源疫苗中的可能作用进行了讨论。 相似文献
83.
Cortajarena AL Kajander T Pan W Cocco MJ Regan L 《Protein engineering, design & selection : PEDS》2004,17(4):399-409
Protein design aims to understand the fundamentals of protein structure by creating novel proteins with pre-specified folds. An equally important goal is to understand protein function by creating novel proteins with pre-specified activities. Here we describe the design and characterization of a tetratricopeptide (TPR) protein, which binds to the C-terminal peptide of the eukaryotic chaperone Hsp90. The design emphasizes the importance of both direct, short-range protein-peptide interactions and of long-range electrostatic optimization. We demonstrate that the designed protein binds specifically to the desired peptide and discriminates between it and the similar C-terminal peptide of Hsp70. 相似文献
84.
Ruan Ke-He; Milfeld Kent; Kulmacz Richard J.; Wu Kenneth K. 《Protein engineering, design & selection : PEDS》1994,7(11):1345-1351
A 3-D model of human thromboxane A2 synthase (TXAS) was constructedusing a homology modeling approach based on information fromthe 2.0 crystal structure of the hemoprotein domains of cytochromeP450BM-3 and P450cam. P450BM-3 is a bacterial fatty acid monooxygenaseresembling eukaryotic microsomal cytochrome P450s in primarystructure and function. TXAS shares 26.4% residue identity and48.4% residue similarity with the P450BM-3 hemoprotein domain.The homology score between TXAS and P450BM-3 is much higherthan that between TXAS and P450cam. Alignment between TXAS andthe P450BM-3 hemoprotein domain or P450cam was determined throughsequence searches. The P450BM-3 or P450cam main-chain coordinateswere spplied to the TXAS main chain in those sements where thetwo sequences were well aligned. These segments were linkedto one another using a fragment search method, and the sidechains were added to produce a 3-D model for TXAS. A TXAS substrate,prostaglandin H2 (PGH2) was docked into the TXAS cavity correspondingto the arachidonic acid binding pocket in P450BM-3 or camphorbinding site in P450cam. Regions of the heme and putative PGH2binding cavities in the TXAS model were identified and analyzed.The segments and residues involved in the active-site pocketof the TXAS model provide reasonable candidates for TXAS proteinengineering and inhibitor design. Comparison of the TXAS modelbased on P450BM-3 with another TXAS model based on the P450BM-3with another TXAS model based on the P450cam structure indicatedthat P450BM-3 is a more suitable template for homology modelingof TXAS. 相似文献
85.
Markland William; Pollock Daniel; Livingston David J. 《Protein engineering, design & selection : PEDS》1989,3(2):111-116
The interactions between tPA domains that are important forcatalysis are poorly understood. We have probed the functionof interdomain interactions by generating tPA variants in whichdomains are duplicated or rearranged. The proteins were expressedin a transient mammalian expression system and tested in vitrofor their ability to activate plasminogen, induce fibrinolysisand bind to a forming fibrin clot. Duplication of the heavychain domains of tPA produced enzymatically active tPA variants,many of which demonstrated similar in vitro amidolytic and fibrinolyticactivity and similar fibrin affinity to the parent molecule.Zymographic analysis of the domain duplication tPA variantsshowed one major active species for each variant. Selectionof the residues duplicated and the interdomain spacing werefound to be critical considerations in the design of tPA variantswith duplicated domains. We also rearranged the domains of tPAsuch that kringle 1 replaced the second kringle domain and viceversa. An analysis of these variants indicates that the firstkringle domain can confer fibrin affinity to a tPA variant andfunction in place of kringle 2. Therefore, in wild-type tPA,the functions of kringle 1 and kringle 2 must be dependent partiallyon their orientation within the heavy chain of the protein.The functional autonomy of the heavy and light chains of tPAis demonstrated by the activity of a tPA variant in which theorder of the heavy and light chains was reversed. 相似文献
86.
87.
88.
目的获得高质量的中国流行株HIV-1B/C重组亚型包膜蛋白抗原。方法改造表达载体,以构建中国流行株HIV-1B/C重组亚型包膜蛋白基因带有筛选标记的真核细胞表达质粒,将所构建质粒转染真核细胞,用含有抗性的培养基筛选出能够高效、持续表达包膜蛋白的稳定表达细胞株。结果所构建的表达载体pVRPEnv转染细胞后可表达目的蛋白,并建立了稳定表达细胞系。结论获得了可以高效、持续稳定表达中国流行株HIV-1B/C重组亚型包膜蛋白的细胞株。 相似文献
89.
Nielsen Per F.; Roepstorff Peter; Clausen Ib G.; Jensen Ejner B.; Jonassen Ib; Svendsen Allan; Balschmidt Per; Hansen Finn B. 《Protein engineering, design & selection : PEDS》1989,2(6):449-457
Californium-252 plasma desorption mass spectrometry (PDMS) hasbeen employed for the characterization of a series of humaninsulin derivatives in order to evaluate the performance ofthis technique as an analytical tool in protein engineering.Several of the characterized modifications result in a 1 a.m.u.mass change. The precision in mass determination obtainableby PDMS analysis is not sufficient for unambiguous verificationof such modifications based on the molecular weight alone. Itis, however, possible to carry out in situ enzymatic digestionof the sample. Subsequent PDMS analysis will in most cases revealif the modification has been introduced as intended. 相似文献
90.
Bates Paul A.; McGregor Malcolm J.; Islam Suhail A.; Sattentau Quentin J.; Sternberg Michael J.E. 《Protein engineering, design & selection : PEDS》1989,3(1):13-21
A predicted three-dimensional structure of the two N-terminalextracellular domains of human CD4 antigen, a cell surface glycoprotein,is reported. This region of CD4, particularly the first domain,has been identified as containing the binding region for theenvelope gp120 protein of the human immuno-deficiency virus.The model was predicted based on the sequence homology of eachdomain with the variable light chain of immunoglobulins. Theframework ß-sheet regions were taken from the crystalcoordinates of REI. For one region in the first domain of CD4there was an ambiguity in the alignment with REI and two alternatemodels are presented. Loops connecting the framework were modeledfrom fragments selected from a database of main chain coordinatesfrom all known protein structures. Residues identified as involvedin binding gp120 have been located in several other studieswithin the first domain of CD4. Epitopes from eight monoclonalantibodies have been mapped onto residues in both domains. Competitionof these antibodies with each other and with gp120 can be interpretedfrom the structural model. 相似文献