Reduction of Levels of Volatile Components Associated with the "Beany" Flavor in Soymilk by Lactobacilli and Streptococci

ABSTRACT: Methanol, acetaldehyde, ethanol, and hexanal were the 4 major volatiles detected in unfermented soymilk. Eight of the cultures of lactobacilli or streptococci completely eliminated hexanal in the soymilk during fermentation. However, there were considerable variations in the effects of the cultures on the other 3 compounds. All 8 caused significant reduction in levels of methanol. Streptococcus thermophilus OSU-2 was the only culture that significantly lowered the concentration of ethanol in the soymilk. All except Lactobacillus acidophilus C19 and Lactobacillus casei E5 significantly lowered the level of acetaldehyde. Of the cultures tested, L. acidophilus L1 offered the best potential for producing fermented soymilk with an improved volatile profile.     

Effect of Soybean Varieties and Growing Locations on the Flavor of Soymilk

ABSTRACT: The volatile compounds of soymilk prepared from 5 soybean varieties grown in 2 locations were isolated and separated by a dynamic headspace analyzer and capillary gas chromatography. The coefficient of variation for the quantitative analysis of volatile compounds of soymilk by dynamic headspace analysis was less than 3.0%. Soybean varieties and growing locations had significant effects on the volatile compounds of the batches of soymilk at α= 0.05. The higher the soybean protein, the higher was the volatile compounds of soymilk. Correlation coefficient between the protein content and the volatile compounds of the milk was 0.99. Thirty-eight volatile compounds of soymilk were identified by a combination of mass spectrometry and gas chromatographic retention times of standard compounds. Most of the identified compounds were formed by the lipid oxidation of soymilk. However, 2-pentylfuran, dimethyl disulfide, and dimethyl sulfide were formed by singlet oxygen. The beany or green flavor that makes the soymilk unpleasant or unacceptable to Westerners may be due to 2-pentylfuran, which is formed from linoleic acid by singlet oxygen. The beany flavor can be eliminated by processing the soymilk with a minimum exposure to light and air.     

Stability of β-glucosidase Activity Produced by Bifidobacterium and Lactobacillus spp. in Fermented Soymilk During Processing and Storage

ABSTRACT: Microorganisms possess endogenous enzymes, however the stability of these enzymes during storage in soymilk has not been studied. β-glucosidase is an important enzyme that could be used in the bioconversion of the predominant soy isoflavone glucosides to their bioactive aglycone forms. Fifteen probiotic microorganisms including bifidobacterium, Lactobacillus acidophilus , and Lactobacillus casei were screened for β-glucosidase activity using p-nitrophenyl-β-d-glucopyranoside as a substrate. Six strains were selected on the basis of β-glucosidase activity produced during fermentation of soymilk. The stability of the enzyme activity was assessed during incubation for up to 48 h and storage for 8 wk at frozen (-80°C), refrigerated (4°C), room (24.8°C), and incubation (37°C) temperatures. L. casei strains showed the highest β-glucosidase activity after 24 h of incubation followed by L. acidophilus strains, whereas bifidobacterium strains showedleast activity. However, p-glucosidase from Bifidobacterium animalis BB12 showed the best stability during the 48 h fermentation. Lower storage temperatures (-80°C and 4°C) showed significantly higher ( P < 0.05) β-glucosidase activity and better stability than that at higher temperatures (24.8°C and 37°C). The stability of β-glucosidase from these microorganisms should be considered for enzymic biotransformation during storage of isoflavone β-glucosides to bioactive isoflavone aglycone forms with potential health benefits.     

豆浆前处理工艺对其粒径分布及感官品质影响的研究

利用激光衍射粒度分析仪考察了离心、过滤、胶体磨处理、均质、热处理对豆浆稳定性及粒径分布的影响,并通过感官评价方法对豆浆品质进行了评定。结果表明,离心处理对粒径影响最明显,3000r/min,10min离心可使D[4,3]减小为0.487μm,沉淀率仅为0.44%;250目过滤则仅对粒径大于160μm的颗粒有效;均质作用比胶体磨处理效果更明显;豆浆经90℃,15～20min热处理最适;感官评价结果基本反映豆浆粒径分布状态的差异。     

Functionality of soymilk powder and its components in fresh soy bread

ABSTRACT:  The physicochemical changes upon addition of soymilk powder (SMP) to soy bread were investigated. Two-pound loaves of soy bread were produced with components (soluble fiber [SF], insoluble fiber [ISF], soy protein) that mimic those levels contributed by SMP. Soy flour and soy flour/SMP soy breads served as controls. The following were determined for all breads produced: physical properties (loaf volume, crust, and crumb color); chemical compositions (SF and ISF contents, protein and ash contents); and physicochemical properties (water activity, total moisture content by thermogravimetric analysis [TGA], "freezable" water [FW], "unfreezable" water [UFW] content by DSC, stiffness at 25 °C by dynamic mechanical analysis [DMA], and firmness with Instron testing machine). SMP contained significant amounts of SF aside from the ISF fraction and mostly denatured soy protein. SMP addition to soy bread formulation significantly decreased loaf volume with respect to control soy bread, which can be attributed to the ISF and SPI contents of this ingredient. Other effects of SMP were found to be lighter and yellowish crumb color, darker crust color, and increase in firmness, as well as no change in moisture content, FW and UFW contents, water activity, and stiffness parameters.     

高静压技术对豆浆品质的影响

本文研究发现,400MPa以上的高静压能有效地灭活豆浆中的微生物,当压力为400—600MPa、保压时间为0—20min时,豆浆中的菌落总数、霉菌和酵母数分别降低0.51-3.69、0.31-2.83个对数级。其中经600MPa、20min处理,豆浆中的菌落总数不能检出。HHP处理后豆浆的色泽无明显变化(ΔE<2);pH略有升高(P<0.05);表观粘度增加,其流变特性指数n有所减小,粘稠系数K有所增大,但是HHP处理对K和n值的影响均小于巴氏杀菌处理的豆浆,HHP处理较巴氏杀菌能更好地保证食品的品质。     

Germination improves the functional properties of soybean and enhances soymilk quality

The effects of soybean variety and germination time on structural changes, antinutritional factor content, antioxidant activity of germinated soybean, and on the functional properties of soymilk were comprehensively investigated. The results showed that the antioxidant activity increased with increasing germination time. The content of antinutritional factors decreased with increasing germination time. Soybean varieties with the lowest tannin and trypsin inhibitor content were DN690 and HJ1. Lipoxygenase activity and phytic acid content showed no significant differences among soybean varieties. The content of α-helices and β-turns in soybean protein decreased with increasing germination time, while the content of β-sheets was increased. Soybean protein was progressively broken down into smaller molecular peptides during the germination process. The digestion and content of soluble protein in soymilk increased with germination. In summary, we show that germination is an effective, cheap, and green method that improves the functional properties of soybean and soymilk.     

Protein quality and antioxidant properties of soymilk derived from black soybean after in vitro simulated gastrointestinal digestion

Protein quality and antioxidant properties of soymilk derived from black soybean (eight varieties) in China were analysed following in vitro simulated gastrointestinal digestion (including dialysis). Soymilk from black soybean possessed high okara weight but low yield, protein content and sensory scores. The in vitro digestibility of protein in all black soymilk samples was higher than 60%, and the Shenmu black soybean exhibited the highest digestibility. Non-digested milk from the black soybean exhibited significantly high total phenolic content (TPC) (127.15–173.04 mg/100 mL), ferric-reducing antioxidant power (FRAP) (272.18–366.27 μmol L⁻¹) and DPPH free radical-scavenging activity (61.20–83.81%). These parameters were significantly lower in the non-digested soymilk than those in soymilk after gastric digestion but higher than those of soymilk in the dialysed fraction. Gastric digestion significantly increased bioactive compound levels released from black soymilk, and the bioaccessibility of phenolic compounds was 24.37–36.05%. Hence, black soymilk was sufficiently available for human absorption.     

Kinetics of sensory quality changes in soymilk during thermal processing,by parametric and non‐parametric data analyses

Soymilk was subjected to various heat treatments at 90, 120 and 140 °C for various lengths of time. The colour and flavour of the soymilk samples were evaluated by a sensory panel consisting of eight semi‐trained assessors on (1) a 9‐unit hedonic scale, with analysis based on the assumption that the units are equidistant, and (2) a 15‐point hedonic ‘Smiley’ pictorial scale, the results being analysed non‐parametrically. The proportion (%) of ratings higher than or equal to/higher than the overall median of the samples was used as a rigorous estimate of product quality. These values were used to determine the colour and flavour changes in heated soymilk and their temperature dependence. The Z‐values derived from the parametric and non‐parametric analyses were in close agreement, ranging from 31 to 33 °C and from 30 to 32 °C for colour and flavour respectively. © 2000 Society of Chemical Industry     

Insolubilisation and gelation of heat–frozen soymilk

Soymilk prepared from soybean seed was heated and frozen. After thawing, precipitation was shown to occur in soymilk. Precipitation from heated and frozen soymilk increased with increase in heating time. Precooling treatment (−5°C) before freeze‐storage of heated soymilk resulted in gel‐like solidification of soymilk. The gel strength of the freeze‐gel formed from soymilk was related to the heating time and the viscosity of the soymilk before freezing. © 1999 Society of Chemical Industry