Banana peel: an effective biosorbent for aflatoxins

This work reports the application of banana peel as a novel bioadsorbent for in vitro removal of five mycotoxins (aflatoxins (AFB1, AFB2, AFG1, AFG2) and ochratoxin A). The effect of operational parameters including initial pH, adsorbent dose, contact time and temperature were studied in batch adsorption experiments. Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and point of zero charge (pH_pzc) analysis were used to characterise the adsorbent material. Aflatoxins’ adsorption equilibrium was achieved in 15 min, with highest adsorption at alkaline pH (6–8), while ochratoxin has not shown any significant adsorption due to surface charge repulsion. The experimental equilibrium data were tested by Langmuir, Freundlich and Hill isotherms. The Langmuir isotherm was found to be the best fitted model for aflatoxins, and the maximum monolayer coverage (Q₀) was determined to be 8.4, 9.5, 0.4 and 1.1 ng mg^?1 for AFB1, AFB2, AFG1 and AFG2 respectively. Thermodynamic parameters including changes in free energy (ΔG), enthalpy (ΔH) and entropy (ΔS) were determined for the four aflatoxins. Free energy change and enthalpy change demonstrated that the adsorption process was exothermic and spontaneous. Adsorption and desorption study at different pH further demonstrated that the sorption of toxins was strong enough to sustain pH changes that would be experienced in the gastrointestinal tract. This study suggests that biosorption of aflatoxins by dried banana peel may be an effective low-cost decontamination method for incorporation in animal feed diets.     

Astringency sub-qualities of red wines and the influence of wine–saliva aggregates

Astringency is a sensory attribute, related to the quality and mouthfeel of red wines. However, the origin of astringency sub-qualities, such as the typical drying astringency found in immature grapes, is still unknown. Astringency of red wines with similar tannin content but different astringency sub-qualities, from different harvest dates, is studied. Astringency was characterised in terms of friction coefficient, polyphenol content, sensory analysis and tannin/salivary–proteins aggregates characterisation. A different evolution during ripening was found for both Cabernet Sauvignon and Carménère, and tannin–protein aggregates showed differences in size, shape and surface. The velvety sub-quality appears to be related to aggregates with low precipitation, and with specific surface characteristics as roundness and Feret diameter. Results from this work propose an effect of aggregates on sensory perception and opens the possibility to explore their effect on oral lubrication.     

Climate and Bordeaux wine quality: identifying the key factors that differentiate vintages based on consensus rankings

The climate of Bordeaux, France, was examined to determine if climatic factors can distinguish between consensus vintage rankings, developed using eight ratings sources, of red and sweet white wines from 1961 to 2009. Climate variables were computed for the growing season and average plant phenological stages and were compared between the 10 highest and lowest ranked vintages. Good vintages exhibited higher heat accumulation during the growing season and a general lack of rainfall, particularly during veraison. Most climate factors were consistent for both red and sweet white wines. Mean maximum temperature during the growing season was an important discriminator between good and poor vintages for both reds and whites, although sweet white wines were also affected by growing season precipitation and temperatures during the vine's dormant period. In general, consensus vintage quality is consistent between reds and whites (Spearman's ρ?=?0.66, p?相似文献   

A finite element simulator for spine orthopaedics with haptic interface

The human spine is a biomechanical structure that allows complex motions while providing stability and protection for the spinal cord during a variety of loading conditions. In this study, finite element models of spine are developed to investigate clinical problems as well as to predict its biomechanical behaviour. This research proposes a combination of an online real-time FE simulator and an offline nonlinear FEA solver. Haptic feedback is provided in the online FE simulator to enhance the human–computer interaction of the system. Primitive results of spinal deformation can be obtained from the haptic online FE simulator. The offline FEA solver provides detailed deformation and strain/stress information based on the primary simulation results from the online FE simulator.     

Prediction models for the cross-sectional areas of lower lumbar intervertebral discs and vertebral endplates

Current approaches to obtain lumbar morphometry data usually require expensive medical imaging technology, long processing time, and are often limited by small sample size. This study develops regression models for the cross-sectional areas (CSAs) of the lower lumbar (i.e., from L3/L4 to L5/S1 level) intervertebral discs (IVDs) and vertebral endplates (EPs) using both simple and complex anthropometric variables. CSAs were measured using OsiriX^© software, based on 3T magnetic resonance imaging (MRI) scans from a sample of 13 females and 22 males, aged between 20 and 40, and asymptomatic of low back disorders. Comprehensive body anthropometry data were collected and included in the regression analyses. Several multiple regression models were developed with varying levels of complexity. Subject stature, elbow dimensions, and ankle dimensions were statistically significant predictors for the CSAs of IVDs and EPs. Gender exhibited a more predictive relationship with the CSAs when compared to body weight and age. In general, regression models using newly proposed best subset procedure resulted in smaller prediction errors, compared to the models using easy-to-measure variables (i.e., gender, age, height, and weight). However, simple regression models are still worthy of investigation given the low cost, ease of data collection, and satisfactory model performance.     

Enhancement of biohydrogen production from sustainable orange peel wastes using Enterobacter species isolated from domestic wastewater

Five facultative anaerobic bacterial isolates were recovered from domestic wastewater. These isolates were identified based on the 16S rRNA as Enterobacter aerogenes (one isolate), Enterobacter cloacae (two isolates), and Cronobacter sakazakii (three isolates). These isolates were examined for their potential to evolve hydrogen on a glucose medium. The most potent hydrogen‐producing isolates, E aerogenes (KY549389) and E cloacae (KY524293), were examined for their capacity to generate hydrogen, acetone, butanol, and ethanol using orange peel (OP) hydrolysate. OP powder was pretreated with n‐hexane to remove the toxicity of d ‐limonene. Different concentrations (4%, 6%, and 8% w/v) of limonene‐free OP were subjected to the boiling water (temperature of 100°C) or acid (HCl) treatments. The maximum fermentative H₂ production of 1700 and 1620 mL/L was obtained from 6% OP hydrolysate extracted with boiling water using facultative anaerobic E aerogenes (KY549389) and E cloacae (KY524293), respectively. Hydrogen production efficiency was 0.99 and 1.19 mol H₂/mol glucose for E aerogenes and E cloacae, respectively. The total fermentative acetone, butanol, and ethanol (ABE) generated by E aerogenes and E cloacae were 0.78 and 0.38 g/L including acetone (0.05 and 0.04 g/L), butanol (0.011 and 0.013 g/L), and ethanol (0.71 and 0.32 g/L), respectively. The maximum ABE productivity was 0.01 and 0.005 g/L/h generated at 60 g/L OP hydrolysate by E aerogenes and E cloacae, respectively. These strains were positive for nitrogen fixation (nitrogenase) capability estimated by the acetylene reduction assay. Application of OP hydrolysate without the addition of any nutritional components or reducing agent is considered an eco‐friendly, economical, and commercial substrate for desired biofuel production.     

Layer thickness and draft angle selection for stereolithography injection mould tooling

The introduction of rapid prototyping has allowed engineers and designers to generate physical models of required parts very early on in the design and development phase. Further to this, the use of stereolithography (SL) cavities as a rapid tooling method has allowed plastic prototype parts to be produced in their most common production manner -- by injection moulding. The process is best suited to small production runs where the high costs of conventionally machined tooling is prohibitive. One of the major drawbacks of the SL injectionmoulding process is the susceptibility of the tools to premature failure. SL tools may break under the force exerted by part ejection when the friction between a moulding and a core is greater than the tensile strength of the core, resulting in tensile failure. Very few justified recommendations exist about the choice of mould design variables that can lower the part ejection force experienced and reduce the risk of SL tool failure. This research investigates the ejection forces resulting from SL injection moulding tools which are identical in all respects except for their build layer thickness and incorporated draft angles in an attempt to identify appropriate evidence for recommendations with respect to these design variables and SL injection moulding. The results show that adjustment of draft angle results in a change of part ejection force as a reasonably linear relationship. An adjustment of the build layer thickness results in a change in part ejection force as a more non-linear relationship. The adjustment of build layer thickness had a greater effect on ejection force than the adjustment of draft angle. The results also show that the surface roughness of all tools remains unchanged after moulding a number of parts in polypropylene. A mathematical model was used in an attempt to predict ejection forces according to the moulding material used. This model reflected the experimental results in terms of relative values but not in absolute values, which may be due to inappropriate specific values used in their calculation. Finite element analysis (FEA) was used in an attempt to identify the factors involved in the ejection process. Results indicate that the effect of draft angle on ejection force is due to elastic deformation of the surface roughness. A fact borne out by the lack of damage to the surface after ejection.     

Curved muscles in biomechanical models of the spine: a systematic literature review

Abstract

Early biomechanical spine models represented the trunk muscles as straight-line approximations. Later models have endeavoured to accurately represent muscle curvature around the torso. However, only a few studies have systematically examined various techniques and the logic underlying curved muscle models. The objective of this review was to systematically categorise curved muscle representation techniques and compare the underlying logic in biomechanical models of the spine. Thirty-five studies met our selection criteria. The most common technique of curved muscle path was the ‘via-point’ method. Curved muscle geometry was commonly developed from MRI/CT database and cadaveric dissections, and optimisation/inverse dynamics models were typically used to estimate muscle forces. Several models have attempted to validate their results by comparing their approach with previous studies, but it could not validate of specific tasks. For future needs, personalised muscle geometry, and person- or task-specific validation of curved muscle models would be necessary to improve model fidelity.

Practitioner Summary: The logic underlying the curved muscle representations in spine models is still poorly understood. This literature review systematically categorised different approaches and evaluated their underlying logic. The findings could direct future development of curved muscle models to have a better understanding of the biomechanical causal pathways of spine disorders.