螺旋藻水溶性多糖的分离纯化及其本征特性

螺旋藻干粉经乙醇脱脂后用水提取,用乙醇沉淀,并经凝胶柱层析精制,获得了３ 种平均相对分子质量分别为１５０００、２１００ 和４４６００ 的均一螺旋藻精制多糖ＳＰＡ １、ＳＰＡ ２ 和ＳＰＡ ３（ＳＰＡ为螺旋藻粗多糖Ａ的简称）,经酸水解,高效液相色谱分析,ＳＰＡ １ 由Ｌ 鼠李糖、Ｄ 木糖、Ｄ 葡萄糖和Ｄ 半乳糖组成,其质量分数分别为２７－２２％ 、１５．９５％ 、３８．２２％ 和１８．６１％ ;ＳＰＡ ２ 由Ｌ－ 鼠李糖、Ｄ 木糖和Ｄ 葡萄糖组成,其质量分数分别为２５％ 、９．５％ 和６５．５％ ;ＳＰＡ ３ 由Ｌ 鼠李糖和Ｄ 葡萄糖组成,其质量分数分别为１５．１４％ 和８４．８６％ 。经红外光谱分析,ＳＰＡ １、ＳＰＡ ２ 均以糖残基通过β苷键连接而成;ＳＰＡ ３ 以糖残基通过α苷键连接而成     

螺旋藻多糖对环磷酰胺所致小鼠骨髓抑制的实验研究

目的 研究螺旋藻多糖对小鼠骨髓造血功能的增强作用。方法 将小鼠分成4 组:对照组、环磷酰胺模型组、螺旋藻多糖30 mg·kg^-1、螺旋藻多糖60mg·kg^-1。模型组、螺旋藻多糖组腹腔注射环磷酰胺造模。同时螺旋藻多糖组灌胃螺旋藻多糖, 对照组、模型组灌胃生理盐水。结果 螺旋藻多糖在30、60mg·kg^-1剂量下, 对环磷酰胺所致小鼠外周血象、骨髓有核细胞均有改善(P <0.01) 。对骨髓细胞微核增加有明显的拮抗作用(P <0.05) 。螺旋藻多糖组小鼠骨髓细胞染色体畸变率较模型组显著降低,其抑制率分别为51.2%、61.5 %。结论 螺旋藻多糖可抑制环磷酰胺诱发的小鼠造血功能损伤。     

螺旋藻培养液中碳、氮、磷的优化及其效应评价

以总蛋白含量和比生长速率为评价指标,进行综合评分后,优化了螺旋藻培养液中碳、氮、磷源的种类和浓度,其中,碳源为NaHCO37.00g/L、氮源为尿素0.10g/L、磷源为KH2PO40.10g/L。与对照Zarrouk配方相比,该优化配方不但可以降低螺旋藻的生产成本,而且显著提高了螺旋藻总蛋白含量、色素蛋白含量和类胡萝卜素含量。     

藻蓝蛋白对Au（Ⅲ）原位还原与纳米Au（0）形成动态过程的谱学研究

在4℃、避光条件下,将藻蓝蛋白（PC）与AuCl3作用,通过UV、FS、FT-IR等谱学方法研究了PC与Au（Ⅲ）作用的动态过程,结果显示：PC在615nm的特征吸收峰强度明显减弱,并随Au（Ⅲ）浓度增加和时间延长单调降低;PC的荧光发射峰和荧光激发峰也均呈现衰减趋势.同时通过TEM观察到纳米Au（0）粒子形成,纳米金基本呈球形,有较窄的分布尺寸,粒径在20 nm左右,均匀地分散于PC中,这是PC原位还原Au（Ⅲ）所形成的.     

螺旋藻巧克力的研制

研究在巧克力液块中添加螺旋藻干粉等物质,制成营养均衡的螺旋藻巧克力,确定最佳配方为螺旋藻干粉5％、砂糖30％、卵磷脂0．3％和单硬脂酸甘油酯0．4％的比例添加。并对螺旋藻巧克力的营养成分及理化微生物指标进行测定分析。     

Hydrogen production from dry spirulina algae with downstream feeding in microwave plasma reactor assisted under atmospheric pressure

Advancement in the field of biomass and bioenergy has pulled in researchers to produce biofuels derived from naturally accessible feedstock by adopting diverse innovative approaches in biomass pretreatment. In this study, pyrolysis of micro algae with downstream processing of biomass from the resonator cavity under atmospheric pressure was conducted and hydrogen production produced under different microwave power was analyzed. With the increase in microwave power, the hydrogen gas yield accounted to be as twice as that at lower power, where the concentration of produced hydrogen accounts for about 30.80%, 33.20%, and 37.58% at microwave power levels of 800, 900 and 1000 W. It was also observed that no methane was produced in this study since most of the methane produced from microwave plasma conversion has reacted with CO₂ and produced CO and H₂, hence dropped in the concentration of CO₂ with decline in power intensity.     

钝顶螺旋藻中藻蓝蛋白提取纯化新工艺

用０３ｍｍｏｌ／Ｌ的十二烷基苯磺酸钠提取钝顶螺旋藻中的藻蓝蛋白,提取率达到了９８％。再在硅藻土５４５柱上分级洗脱,进一步经ＤＥＡＥ—纤维素柱纯化,其纯度达到了４１（指可见区最大吸收与２８０ｎｍ处之比）,最大吸收峰在６１９ｎｍ,室温荧光发射峰在６４３ｎｍ。     

螺旋藻片剂生产中实施GMP标准探讨

根据《保健食品生产规范》,参照《药品生产质量规范》要求,结合螺旋藻生产实际,论述螺旋藻片剂实施GMP标准的硬件和软件要求,以保证螺旋藻片剂生产质量。     

Evaluation of the influence of spirulina microalgae on the drug delivery characteristics of genipin cross-linked chitosan hydrogels

Abstract

Chitosan-spirulina hydrogels were prepared by crosslinking of chitosan with genipin, in presence of spirulina microalgae. The swelling ratios and kinetic parameters of the hydrogels in distilled water, pH:2 and pH:7.4 solutions and the drug 5-Fluorouracil delivery capacities of the hydrogels were determined by gravimetric and spectrophotometric analyses, respectively and the results were statistically evaluated. The morphological structures, thermal and mechanical properties of the hydrogels were investigated using related techniques. Furthermore, cytotoxicity test was performed for assessment of biocompability of the hydrogels.The all results indicate that spirulina significantly improves the drug delivery properties of the genipine cross-linked hydrogels.     

螺旋藻多糖粗提的新方法工艺条件研究

开发了一种提取螺旋藻多糖的新方法——超声提取和冷碱液提取相结合的方法。正交实验确定的最佳提取条件为:温度为常温,超声时间1 h,提取时间2 h,溶液的pH值为9,固液比9∶1。在此条件下,提取率较传统方法提高5%左右。