Early Osteogenic Marker Expression in hMSCs Cultured onto Acid Etching-Derived Micro- and Nanotopography 3D-Printed Titanium Surfaces

Polyetheretherketone (PEEK) titanium composite (PTC) is a novel interbody fusion device that combines a PEEK core with titanium alloy (Ti6Al4V) endplates. The present study aimed to investigate the in vitro biological reactivity of human bone-marrow-derived mesenchymal stem cells (hBM-MSCs) to micro- and nanotopographies produced by an acid-etching process on the surface of 3D-printed PTC endplates. Optical profilometer and scanning electron microscopy were used to assess the surface roughness and identify the nano-features of etched or unetched PTC endplates, respectively. The viability, morphology and the expression of specific osteogenic markers were examined after 7 days of culture in the seeded cells. Haralick texture analysis was carried out on the unseeded endplates to correlate surface texture features to the biological data. The acid-etching process modified the surface roughness of the 3D-printed PTC endplates, creating micro- and nano-scale structures that significantly contributed to sustaining the viability of hBM-MSCs and triggering the expression of early osteogenic markers, such as alkaline phosphatase activity and bone-ECM protein production. Finally, the topography of 3D-printed PTC endplates influenced Haralick’s features, which in turn correlated with the expression of two osteogenic markers, osteopontin and osteocalcin. Overall, these data demonstrate that the acid-etching process of PTC endplates created a favourable environment for osteogenic differentiation of hBM-MSCs and may potentially have clinical benefit.     

生态系统服务功能损害调查与价值量化方法研究

随着国家对生态环境保护的大力推进,开展生态系统服务功能损害鉴定评估,可以切实打击环境污染和生态破坏行为,提升污染环境和破坏生态者的违法成本.主要通过对各种生态系统提供的服务功能进行整理,依据中国现行的生态环境损害鉴定评估相关技术规范和方法对生态系统服务功能的调查和评价进行了程序、内容、方法上的研究分析,同时选取森林生态...     

热暴露对SiC纤维增强Ti基复合材料基体织构的影响

本文利用电子背散射衍射（EBSD）技术,研究了高温热暴露对SiC纤维增强Ti-6A1—4V复合材料基体织构的影响。结果表明,相对于制备态复合材料较为集中的晶粒取向,900℃10h热暴露后的复合材料其晶粒生长的方向既有基面方向,又有棱面方向,同时还存在锥面方向。随着热暴露时间的延长,900℃75h热暴露的复合材料基面方向的生长方式与900℃10h热暴露的复合材料情况恰好相反。另外,无论是基面方向还是棱面方向,随着热暴露时间的延长,晶粒生长的取向均有向单一取向演化的趋势,且锥面方向上的生长会逐渐消失。     

The Role of the Choroid in Stargardt Disease

Stargardt disease is the commonest juvenile macular dystrophy. It is caused by genetic mutations in the ABCA4 gene. Diagnosis is not always straightforward, and various phenocopies exist. Late-onset disease can be misdiagnosed with age-related macular disease. A correct diagnosis is particularly critical because of emergent gene therapies. Stargardt disease is known to affect retinal pigment epithelium and photoreceptors. Many studies have also highlighted the importance of the choroid in the diagnosis, pathophysiology, and progression of the disease. The choroid is in an integral relationship with the retinal pigment epithelium and photoreceptors, and its possible involvement during the disease should be considered. The purpose of this review is to analyze the current diagnostic tools for choroidal evaluation and the extrapolation of useful data for ophthalmologists and researchers studying the disease.     

Proteomic Biomarkers of the Apnea Hypopnea Index and Obstructive Sleep Apnea: Insights into the Pathophysiology of Presence,Severity, and Treatment Response

Obstructive sleep apnea (OSA), a disease associated with excessive sleepiness and increased cardiovascular risk, affects an estimated 1 billion people worldwide. The present study examined proteomic biomarkers indicative of presence, severity, and treatment response in OSA. Participants (n = 1391) of the Stanford Technology Analytics and Genomics in Sleep study had blood collected and completed an overnight polysomnography for scoring the apnea–hypopnea index (AHI). A highly multiplexed aptamer-based array (SomaScan) was used to quantify 5000 proteins in all plasma samples. Two separate intervention-based cohorts with sleep apnea (n = 41) provided samples pre- and post-continuous/positive airway pressure (CPAP/PAP). Multivariate analyses identified 84 proteins (47 positively, 37 negatively) associated with AHI after correction for multiple testing. Of the top 15 features from a machine learning classifier for AHI ≥ 15 vs. AHI < 15 (Area Under the Curve (AUC) = 0.74), 8 were significant markers of both AHI and OSA from multivariate analyses. Exploration of pre- and post-intervention analysis identified 5 of the 84 proteins to be significantly decreased following CPAP/PAP treatment, with pathways involving endothelial function, blood coagulation, and inflammatory response. The present study identified PAI-1, tPA, and sE-Selectin as key biomarkers and suggests that endothelial dysfunction and increased coagulopathy are important consequences of OSA, which may explain the association with cardiovascular disease and stroke.     

基于近邻传播聚类的铁路客运节点类别划分

为简化旅客列车开行方案优化编制问题,采用聚类法对铁路客运节点进行类别划分。选取2014年日均旅客发送量排名前100位客运节点的相关属性数据为实例,以铁路客运节点的类别划分依据作为属性变量,首先运用分层聚类中的凝聚法对属性变量进行聚类,然后根据简化的客运节点变量指标,运用近邻传播算法对客运节点样本其进行聚类,并引用CH、KL、IGP等三种聚类有效性指标对聚类结果加以分析。研究结果表明,将100个客运节点分为5个类别时,具有最好的聚类效果,可为旅客列车开行方案的设计奠定基础。     

家用电冰箱碳排放评价指标体系研究

针对家用电冰箱的碳排放评价问题,本文在分析家用冰箱生命周期碳足迹的基础上,引用了调整容积的概念统一冰箱产品的功能描述,并基于产品功能与其生命周期碳排放关键影响因素构建了家用冰箱生命周期碳排放评价指标体系,为设计人员进行电冰箱的低碳设计提供支持。     

Single-Step Fast Tissue Clearing of Thick Mouse Brain Tissue for Multi-Dimensional High-Resolution Imaging

Recent advances in optical clearing techniques have dramatically improved deep tissue imaging by reducing the obscuring effects of light scattering and absorption. However, these optical clearing methods require specialized equipment or a lengthy undertaking with complex protocols that can lead to sample volume changes and distortion. In addition, the imaging of cleared tissues has limitations, such as fluorescence bleaching, harmful and foul-smelling solutions, and the difficulty of handling samples in high-viscosity refractive index (RI) matching solutions. To address the various limitations of thick tissue imaging, we developed an Aqueous high refractive Index matching and tissue Clearing solution for Imaging (termed AICI) with a one-step tissue clearing protocol that was easily made at a reasonable price in our own laboratory without any equipment. AICI can rapidly clear a 1 mm thick brain slice within 90 min with simultaneous RI matching, low viscosity, and a high refractive index (RI = 1.466), allowing the imaging of the sample without additional processing. We compared AICI with commercially available RI matching solutions, including optical clear agents (OCAs), for tissue clearing. The viscosity of AICI is closer to that of water compared with other RI matching solutions, and there was a less than 2.3% expansion in the tissue linear morphology during 24 h exposure to AICI. Moreover, AICI remained fluid over 30 days of air exposure, and the EGFP fluorescence signal was only reduced to ~65% after 10 days. AICI showed a limited clearing of brain tissue >3 mm thick. However, fine neuronal structures, such as dendritic spines and axonal boutons, could still be imaged in thick brain slices treated with AICI. Therefore, AICI is useful not only for the three-dimensional (3D) high-resolution identification of neuronal structures, but also for the examination of multiple structural imaging by neuronal distribution, projection, and gene expression in deep brain tissue. AICI is applicable beyond the imaging of fluorescent antibodies and dyes, and can clear a variety of tissue types, making it broadly useful to researchers for optical imaging applications.     

顾客满意度评价体系的建立及应用

介绍了顾客满意度评价体系的构建,该体系包括评价指标的构成、权重确定、顾客对评价指标态度的量化等,采用主观赋权等方法确定各评价指标的权重,将顾客对评价指标态度分为五个等级,并通过多层加权方法,给各等级相应赋值。通过对莱钢某产品顾客满意度定量测评,说明该评价体系对挖掘顾客价值取向、指导企业经营改进、开拓市场等方面的作用,并对该评价体系的运用作了进一步的探讨与思考。     

储层流动单元研究新技术在油田开发中的应用

流动单元研究提供了精细解剖砂体,表征砂体内部非均质性的新方法。本文在传统流动单元划分的基础上应用流动带指标新方法(F z)进行储层流动单元的定量划分,为合理进行油田开发动态分析提供依据,同时开辟了一个可预测可操作的有效流动单元研究方法。