The Study of Otsu Algorithm Applied in the Measuring of Ash Proportion

To further improve the boiler ash ratio detection methods and resource utilization, through image processing technology for boiler ash ratio analysis, the article first studied the one-dimensional Otsu algorithm, and then for the one-dimensional Otsu algorithm, in order to improve the accuracy of the algorithm, then it puts forward a two-dimensional Otsu algorithm. Finally the two-dimensional Otsu algorithm combined with the one-dimensional Otsu algorithm and the improved Otsu algorithm. By analyzing the improved Otsu algorithm, this paper considers the pixel gray value, neighborhood information, excluding light, noise and the relative efficiency of one-dimensional Otsu algorithm higher accuracy. The relative dimensional Otsu algorithm operating efficiency has been greatly improved. Improved Otsu algorithm in dealing with boiler ash ratio detection has played a very good part in the ecological environment, economic development and some other important aspects.     

不同改良剂对滨海盐渍粉土改良效果的试验研究

采用桶栽试验的方法,研究了基质、稻壳和菌糠三种不同有机质改良剂对滨海盐渍粉土理化性质和西红柿产量的影响。研究结果表明:与不加改良剂(CK)相比,三种有机质改良剂可降低表层土壤干容重22.5%～33.1%,孔隙率增加25.5%～37.7%;土壤的含盐量降低5.05%～24.54%。使用改良剂处理的土壤,碱化度(ESP)、pH、土壤交换性Na+、土壤Na+、K+占阳离子总量和土壤Cl-下降,而土壤Ca2+和SO42-增加。三种改良剂均能显著增加西红柿的产量,增幅在24.05%～34.46%;单果总量整体有所提高。结果表明,基质、稻壳和菌糠对滩涂高钠盐粉土均具有较好的改良效果。综合考虑改良剂的价格、材料获得难易程度,稻壳和菌康是较为适宜的滨海盐渍粉土改良材料。     

0.3%壳聚糖水剂对番茄产量和病害发生的影响

试验发现0.3%壳聚糖水剂对番茄品质和产量的提高有明显的促进作用,其中500倍液可使单果重增加11.1%,产量提高21.7%。同时它可以明显提高番茄对病害的抵抗能力,抑制病害发生,且3次施药效果更佳,500倍液第3次施药后对番茄晚疫病防效达到74.0%,300倍液对绵疫病可达78.2%。300～500倍液壳聚糖对2种病害的防效与50%多菌灵600倍液防效相近。     

番茄喷施“增收保”的增产效果

本文介绍了番茄喷施“增收保”──一种广谱型植物生长调节剂大田试验示范的增产效果．     

Consumer Sensory Analysis of High Flavonoid Transgenic Tomatoes

Tomatoes have ameliorative effects on cardiovascular disease and cancer. In this study, metabolic engineering of flavonoids was utilized to improve the nutritional value of tomatoes by increasing flavonol and anthocyanin content. Total flavonol content was significantly increased in both the peel and flesh using the onion chalcone isomerase (CHI) gene. The Delila (Del) and Rosea1 (Ros1) genes from the snapdragon Antirrhinum majus were concomitantly expressed to produce an anthocyanin‐rich tomato which was purple in color. Sensory evaluation by a panel of 81 untrained consumers revealed no significant difference in liking of color or texture between CHI, Del/Ros1, and wild‐type tomatoes. Consumers reported marginal but significantly higher preference for the flavor and overall liking of CHI tomatoes over Del/Ros1 and wild‐type tomatoes. This study is the first to report the results of sensory tests of transgenic tomatoes by a consumer panel representing the general consuming public.     

Development of non-enzymatic ZnO nanocomposite-based optical sensor for l-lactate detection in tomato samples

l -lactate is a conjugate base of α-hydroxy acid, a key organic chemical in food processing, fermentation, biology and chemical industries. We are reporting for the first time a facile and rapid non-enzymatic nanosensor for l -lactate detection in tomato-based products using 3-aminophenylboronic acid (3-APBA)-added zinc oxide nanoparticles (ZnO NPs). 3-APBA-functionalised ZnO NPs have a selective and stable SPR intensity change at pH 5.5 to 7.5 and forms a reversible ester with bound l -lactate revealing the wavelength shift from λ = 300 nm to 288 nm in UV-VIS absorption spectrometry. In the spiked (2.5–20 mm ) samples of tomato puree, absorption peak increased as per l -lactate addition with the lower detection limit of 3.98 mm . l -lactate binds with a trigonal form of 3-boronate, thereby generating the tetrahedral complex. ZnO NPs stabilise the tetragonal complex. Nanosensor validated with standard HPLC. This method is useful for l -lactate detection and alternates to conventional techniques.     

液相色谱—串联质谱法测定番茄和黄瓜中敌菌灵残留量

样品采用固相萃取小柱净化,以C18色谱柱为分离柱,以甲酸水—乙腈为流动相进行梯度洗脱,经LC-MS/MS检测,以多反应监测模式检测目标化合物的特征离子进行定性定量分析。结果表明:该方法线性范围为0.1～10.0μg/mL,线性相关系数0.999 0,方法的检出限为0.01mg/kg,定量限为0.03mg/kg。在加标水平为0.03,0.10,0.50 mg/kg时,方法的回收率为88.3%～95.2%,RSD在4.5%～8.8%。该方法前处理方式简便、有机溶剂用量少、分析时间短、选择性好且灵敏度高,适用于番茄和黄瓜中敌菌灵的检测。     

番茄PIN基因家族的生物信息学分析及其表达模式

PIN蛋白是生长素输出蛋白,具有多个跨膜结构域,能够介导生长素在整个植物体内的流动。番茄作为一种具有重要经济价值的果蔬,对其生长发育调控的研究具有重要的意义。研究表明,PIN蛋白对植物果实的生长发育起着重要的调控作用。本研究通过生物信息学的方法对番茄PIN基因组中10个SlPINs的核酸和氨基酸序列进行分析,构建番茄Sl PINs的进化树,并分析番茄SlPINs在不同组织和果实生长发育过程中的表达量变化,为进一步研究SlPINs的生物学功能提供理论依据。     

胶体金免疫电镜技术检测番茄环纹斑点病毒

本实验利用胶体金免疫电镜技术检测番茄环纹斑点病毒（ tomato zonate spot virus, TZSV）,分别以TZSV的N 蛋白多克隆抗体及TZSV的Gn蛋白多克隆抗体为一抗,然后以胶体金标记羊抗兔IgG?gold（10 nm）为二抗对病毒进行胶体金标记,电镜观察用TZSV Gn蛋白抗体标记的胶体金颗粒能很好地结合到病毒粒体上。结果表明该方法可以实现对TZSV更为准确可靠地检测。