一株产白藜芦醇真菌的培养及调控研究

在虎杖组织培养过程中分离出1株真菌,对其发酵液提取物进行TLC和HPLC分析证明能产生白藜芦醇,并对其培养条件及白藜芦醇积累特性进行了初步研究,通过正交优化试验得到最佳培养条件为:20g/L蔗糖、2g/L硝酸铵、自然pH值（6．8～7．0）、温度为28℃,转速100r/min。3mmol/L苯丙氨酸前体物、Mg2+、Zn2+能促进真菌B-39的生长及白藜芦醇的积累,其白藜芦醇含量能达8．6617μg/100ml。     

野刺梨营养酒饮料的研制

用酒糟酒、野刺梨果汁、蜂花粉、香精为原料,通过正交试验,探索含酒营养果汁饮料的生产工艺,并对蜂花粉的破壁工艺进行了探讨。实验结果显示,花粉用曲霉培养,在pH5.4、温度55℃、10%NaCl条件下降解56h,破壁率达98.5%以上。由酒糟酒降度实验可知:用1.35mg/100ml海藻酸钠和180mg/100ml可溶淀粉联合处理20°加浆酒,18h可沉淀完全,除去大部分高级脂肪酸酯。     

川北地区黑木耳中锌铁铜微量元素的测定

为了解不同产地黑木耳中金属元素含量是否与产地有关,采用火焰原子吸收分光光度法测定了绵阳、通江、青川、北川等地黑木耳中铜、铁、锌3种微量元素的含量。样品采用干法灰化和湿法消解进行处理。测定结果表明干法效果较好。同时对方法进行了准确度和精密度测定,其加标平均回收率为97%,RSD<3%。结果表明:4种黑木耳都含有丰富的人体必需的微量元素,不同产地黑木耳中同一种元素的含量不同。     

野生葡萄及其F1代副梢遗传规律的研究

对西北农林科技大学野生葡萄种植资源圃61类野生葡萄连续3年调查,结果表明,野生葡萄副梢性状类型分布广泛,副梢长度和形成率变异均不服从正态分布,表现出质量性状特征.主梢长度和副梢长度表现为极显著正相关(R=0.6271,P＜0.0001).以长副梢、短副梢葡萄种类进行杂交的燕山×河岸为例,F1代副梢形成率呈连续性变异,表现出数量性状遗传特征;副梢长度变异不服从正态分布,表现出质量性状遗传特征.说明中国野生葡萄和美洲种葡萄在副梢长度和形成率遗传方面存在差异.     

干型山葡萄酒酿造试验

用广西都安野生毛葡萄与西北农大提供的优质野葡萄品系（西农2号）进行嫁接，用该嫁接品种进行干型山葡萄酒酿造试验，产品质量达到中档水平，但有微咸味，酒中总酸实际中降至7．8g/L，与理论降酸量7．0g/L尚有差距，有待探索。     

Determination of water quality variables, endotoxin concentration, and Enterobacteriaceae concentration and identification in southern High Plains dairy lagoons

The objectives of this study were to determine the concentration of endotoxin, determine 20 water quality variables, and identify and enumerate fungal and bacterial pathogens from United States southern High Plains dairy lagoons and control lakes during summer and winter. Water samples were collected in triplicate from the north, south, east, and west quadrants of each body of water. The mean (± SEM) winter dairy lagoon endotoxin concentration was significantly higher (9,678 ± 1,834 ng/mL) than the summer concentration (3,220 ± 810 ng/mL). The mean endotoxin concentration of the 2 control lakes (summer: 58.1 ± 8.8 ng/mL; winter: 38.6 ± 4.2 ng/mL) was significantly less than that of the dairy lagoons. Two hundred-one Salmonella enterica spp. isolates were identified, 7 serovars were recovered from the dairy lagoons, and 259 Salmonella ssp. were identified from 5 other dairy locations (milk barn, ditch effluent, settling basin, feed alley pad flush, and center pivots). Twenty-eight Salmonella spp. were identified from center pivot water. Escherichia coli O157:H7 pathogens were isolated from other dairy locations but not from lagoons. Neither Salmonella spp. nor E. coli O157:H7 were identified from control lakes. Enterobacteriaceae opportunistic pathogens were isolated from both dairies and control lakes. Important mesophilic and thermophilic catabolic (to manure biosolids) fungal isolates were identified from dairy effluent locations, but no thermophilic fungal isolates were cultured from the control lakes. Adequate curing of green forage following center pivot irrigation is important to kill lagoon water enteric pathogens, even though the lagoon water is mixed with fresh water. Recirculating lagoon water to flush the feed alley pad, where cows stand while eating, to remove manure and using lagoon water to abate dairy dust in loafing pens and unimproved dairy roads is inconsistent with good environmental practice management.     

低糖野生软枣猕猴桃果脯工艺优化的研究

对低糖野生软枣猕猴桃果脯的生产工艺条件进行优化。结果表明,低糖软枣猕猴桃果脯最优工艺条件为:采用8成熟新鲜的或速冻的软枣猕猴桃为原料,沸水烫漂60 s,刺孔,0.04%硫酸铜和0.01%硫酸锌溶液护色、0.05%氯化钙溶液硬化,在50%蔗糖溶液、0.07MPa条件下真空渗糖,在0.09MPa条件下真空干燥。在此优化条件下制备的果脯组织形态较饱满,软硬适中,酸甜适口,具有浓郁的软枣猕猴桃风味。     

丽江产野生青刺果油营养成分分析

目的：综合研究云南丽江地区野生青刺果油的营养成分和功效。方法：采用国内外公认的标准方法进行常规理化、卫生指标及氨基酸、脂肪酸、无机元素等营养成分分析及评价。结果：青刺果油的特性常数为水分及挥发物0.03%、折光指数1.4716(n20)、相对密度0.9260(d2020)、碘值45.91(以I计)、皂化值0.02%、酸价0.17mg KOH/g,过氧化值1.20mmol/g,属于非干性油脂;营养指标为17种氨基酸总量为46.97%,其中含有7种人体必需氨基酸(异亮氨酸、亮氨酸、赖氨酸、蛋氨酸、苯丙氨酸、苏氨酸、缬氨酸),为13.2%,占氨基酸总量的28.1%;富含VA(3.257mg/100g)、VD(1.745 mg/100g)、VE(8.736mg/100g)、VK(0.293mg/100g)、β-胡萝卜素(1.234mg/100g)等;13种矿物质微量元素含量最高的是K,为9.8mg/kg;脂肪酸13种,其中不饱和脂肪酸含量占78.35%,以油酸和亚油酸为主,含量均为38%,饱和脂肪酸、单不饱合脂肪酸和多不饱和脂肪酸组成比例接近于0.7:1:1,油脂营养结构合理,各项指标均符合部分国家食用大豆油标准(GB/T 1535-2003;Codex-Stan 210)。结论：丽江产野生青刺果油属于适合人类食用的天然功能性营养油。     

食用菌软包装保鲜技术研究

研究了软包装技术在食用菌保鲜中的应用。在常温储藏条件下,从保鲜液澄清度变化、保鲜液酸度变化、食用菌色泽变化、食用菌形态变化4个方面来考察不同保鲜液配方的保鲜效果。实验表明:保鲜液1号的保鲜效果较佳,可以与含亚硫酸盐的保鲜液3号效果相当,常温下存放一个月,品质变化不大。     

对山葡萄皮中白藜芦醇的抗氧化性质研究

对山葡萄皮中白藜芦醇进行提取和分离纯化,然后研究山葡萄皮中白藜芦醇的抗氧化性质。主要对白藜芦醇对猪油抗氧化性质的能力和消除氧自由基,羟自由基,超氧自由基的能力进行分析。得出结论:白藜芦醇具有抗氧化能力,随着浓度的增大其消除自由基作用逐渐增强。