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991.
Twelve samples derived from different locations in south central area of China are treated by enrichment and spread-plate
technique for initial screening. Seven chitinase-producing strains are isolated. The chitinase present in the culture supernatant
of strain CS-01 possesses the maximum activity of 0.118 U/mL. Analysis of the morphological feature and the ITS rDNA sequence
reveals that strain CS-01 belongs to Aspergillus fumigatus. Production of the chitinase is regulated by a inducible way and the maximum activity appears at 36 h in colloidal chitin
culture. Purification of the chitinase is carried out by salting out, gel filtrate chromatography and anion exchange chromatography
sequentially. Native-PAGE and SDS-PAGE indicate that the chitinase from A. fumigatus CS-01 is a monomer with the relative molecular mass estimated to be 4.50×104. Its maximum activity appears at pH 5 and 55 °C. The chitinase is stable at pH 4.0–7.5 and below 45 °C.
Foundation item: Projects(50621063, 50674101) supported by the National Natural Science Foundation of China 相似文献
992.
Failure mode and strength anisotropic characteristic of stratified rock mass under uniaxial compressive situation 总被引:1,自引:1,他引:0
A stratified rock mass model was founded by FLAC3D. The failure mode and anisotropic characteristic of strength for stratified rock mass were analyzed. The analysis results
show that the numerical simulation can visually reflect the failure modes of rock samples under different inclination angles
β of structural plane. The stiffness of rock sample before peak strength changes in the compressive procedure. With the increase
of β, the compressive strength σ
c of rock sample decreases firstly and then increases; when β is in the range of 20°–30° and 80°–90°, σ
c has the largest sensitivity to β; while β falls in the range of 30°–70°, σ
c varies little. When ϕ
j
<β<90° (β
j
is friction angle of structure plane), the results obtained from numerical simulation and theoretical analysis are in almost
the same values; while β⩽ ϕ
j
or β=90°, they are in great different values. The results obtained from theoretical analysis are obvious larger than those from
numerical simulation; and the results from numerical simulation can reflect the difference of compressive strength of rock
samples for the two situations of β⩾ ϕ
j
and β=90°, which is in more accordance with the real situation.
Foundation item: Project (50099620) supported by the National Natural Science Foundation of China 相似文献
993.
基于高速摄像机的电磁电器动态特性测试及其图像处理的研究 总被引:1,自引:0,他引:1
为了研究电磁电器动态特性测试的新方法,以智能交流接触器为例拍摄了智能交流接触器的动态过程,对采集的接触器触头、铁心等动态位移图像进行处理,然后应用二进小波多层多尺度分解完成降噪处理,此外本文采用样条求导方式对运动速度进行软测量,从而获得智能交流接触器的真实动态特性,介绍了电磁电器动态特性测试装置与数据处理新方法。该研究采用非接触测试方式,进行图像拍摄与降噪处理,实现了电磁电器动态过程全面、高速与准确的测量,为加快电磁电器产品的研究开发奠定基础。 相似文献
994.
徐青山 《哈尔滨工业大学学报(英文版)》2009,16(3):408-412
With proper phase module transformation,parallel lines can be decomposed to the same directional net and the reverse directional net. The propagation characteristics of traveling waves in the reverse directional net were analyzed,and the refraction coefficient at the fault point for a single phase fault was derived. In addition,the module selection was discussed. Simulation results show that satisfying accuracy can be achieved with the proposed method. Moreover,it is immune to fault types,fault resistances,and outside system parameters. 相似文献
995.
负荷电压静特性模型对潮流计算的影响分析 总被引:1,自引:0,他引:1
在实际系统运行中,采用合理的负荷电压特性模型能较准确地反映电网的潮流分布状况。基于节点负荷的取用功率是其电压的函数,提出采用两种负荷电压静态特性模型,针对不同负荷电压静态特性模型的系数对潮流计算结果的影响,对比分析各关系曲线的斜率从而确定选择负荷电压静态特性模型。所得结果可为电压敏感性负荷的潮流计算提供参考。 相似文献
996.
XU Zhi-wei ZENG Kai-hua WEI Zhou LIU Zhi-qiang ZHAO Xiao-dong TIAN Qiu-hong 《中国矿业大学学报(英文版)》2009,19(1):129-132
Exact calculations of the static earth pressure from a thick alluvium require accurate K0 values. These calculations influence the sinking cost and the safety of the freezing method. The static earth pressure coefficient (K0) of thick and deep soil was analyzed using laboratory tests. The results show that the static earth pressure coefficient of thick and deep soils is nonlinear and different from that of superficial soils. The constant of superficial soils is usually invariant and the total stress or incremental stress definitions used in traditional geo-mechanics give the same value. The influence of load increments when calculating for superficial soil is ignored. The difference in values of K0 for thick alluvium defined by the total stress or the incremental stress methods is over 10%. The effects of the thick alluvium on K0 should be considered during the design of frozen shaft projects. Such things as the frozen shaft thickness and the excavated section height should be chosen to assure the rationality of the design and to avoid potential faults and accidents. 相似文献
997.
Electromagnetic emission (EME) is a kind of physical phenomenon accompanying the process of deformation and frac-ture of loaded coal and rock and it is of importance in quantitatively analyzing its characteristics. This will reveal the process of deformation and fracture of coal and predicting dynamic disasters in coal mines. In this study, the G-P (Grassberger and Procaccia) algorithm, calculation steps of the (if only 1 dimension) correlation dimension of time series and the identification standards of chaotic signals are introduced. Furthermore, the correlation dimensions of EME and the acoustic emission (AE) signals of time series during deformation and fracture of coal bodies are calculated and analyzed. The results show that the time series of pulses number of EME and the time series of AE count rate are chaotic and that the saturation embedding dimensions of a K3 coal sample are, respectively, 5 and 6. The results can be used to provide basic parameters for predicting of EME and AE time series. 相似文献
998.
作为大电机系统中的关键部件,转子的振动特性直接关系到电机工作性能的优劣。为获得转子的振动特性,从转子动力学基本理论出发,应用有限元分析软件ANSYS研究了转子系统的固有频率和振型,对转子结构进行了模态分析,计算出了转子前20阶模态的固有频率,并得到了转子的临界转速,找出了影响转子动力学特性的主要因素,为设计转子的理想工作转速和提高转子的振动特性提供依据。 相似文献
999.
对某景观桥进行了检测评定,提出了3种加固方案,并采用大型通用有限元软件ANSYS建立了桥梁上部结构模型以及3种加固方案的模型,其中加固方案模型建立于根据静载试验数据修正后的模型之上;利用该模型进行了设计荷载工况下的静力分析和模态分析,并根据计算结果比较了3种加固方案以及参数变化对桥梁挠度、支座区最大等效应力、极限承栽力以及动力特性的影响。结果表明:综合采用加固方案1和加固方案3是比较合理的。 相似文献
1000.
高压旋喷桩复合地基承载特性的有限元分析 总被引:1,自引:0,他引:1
结合工程实际,运用大型有限元分析软件ANSYS分析了不同桩长、不同桩土模量比和不同桩间距情况下高压旋喷桩复合地基的承载特性.结果表明,该地基存在有效桩长,当桩土模量较合适时,该地基能够发挥最大作用;同时运用ANSYS优化功能,得出了最优桩长、最优桩体弹性模量和最优桩间距,为实际工程提供一些参考. 相似文献