1-氨基-1-环烷基甲酸的合成

通过改进的Strecker法,分别以环己酮和环戊酮为原料与氨反应生成亚胺（I）,（I）与氰化钠反应生成（Ⅱ）,（Ⅱ）用浓盐酸水解为旷氨基酸（Ⅲ）。反应最佳条件为：环烷基酮、氨水、氯化铵和氰化钠的摩尔比为：1：1．8：1．1：1．1。粗品旷氨基酸用75％的乙醇重结晶。1-氨基-1-环己基甲酸和1-氨基-1-环戊基甲酸的收率分别为56．42％和60．21％。目标化合物用核磁和红外光谱进行了表征。     

尺寸效应对TiC/α-C∶H涂层织构行为的影响

利用磁控溅射法制备了TiC/α-C∶H纳米复合涂层尺寸效应,并对涂层择优取向的影响进行了研究。通过改变涂层中的C含量来调节晶粒尺寸,发现晶粒尺寸对晶粒的择优取向有着重要影响。随着晶粒尺寸减小,TiC的择优取向从面心立方(111)向(200)转变。这种择优取向的尺寸依赖效应可以用热力学来解释。通过计算与尺寸相关的吉布斯自由能解释了尺寸效应与涂层择优取向的关系。     

煤田绳索取芯深孔钻探设备的选择与应用

探讨了煤田绳索取芯深孔钻探设备选用XY-6B立轴岩芯钻机、BW-320泥浆泵、GF-120 kW柴油发电机组及ZT-17A加强型四脚钻塔等,开展了深孔施工技术研究,指出煤田绳索取芯深孔钻探设备可降低钻探直接成本、能占领深孔钻探市场,对提高福建省121队的核心竞争力,保持绳索取芯钻进技术领先水平具有重要意义。     

Tocols of selected spring wheat (Triticum aestivum L.), einkorn wheat (Triticum monococcum L.) and wild emmer (Triticum dicoccum Schuebl [Schrank]) varieties

Wheat contributes significantly worldwide to antioxidant income with beneficial healthy effects. Two varieties of einkorn wheat (Triticum monococcum L. – Escana and Schwedisches Einkorn), two varieties of wild emmer [Triticum dicoccum Schuebl (Schrank)] (Rudico and Kahler Emmer) and three varieties of spring wheat (Triticum aestivum L. – Granny, Kärtner Früher and SW Kadrilj) were evaluated for tocols – tocopherols and tocotrienols by high performance liquid chromatography – electrospray ionisation tandem mass spectrometry (HPLC–ESI/MS/MS). Investigated emmer and einkorn wheat varieties have shown minor lower total tocols content in comparison with spring varieties, however significant typical levels of tocopherols and tocotrienols were found in these groups. In einkorn wheat varieties the major abundance of total tocols is represented by tocotrienols (Schwedisches Einkorn 65.0%, Escana 54.9%), in emmer wheat varieties the abundance of tocotrienols and tocopherols were similar (Rudico 48.9% of tocotrienols, Kahler Emmer 47.6%), whereas in spring bread wheat higher tocopherols (SW Kadrilj 54.5%, Kärtner Früher 60.2%, Granny 55.0%) were recorded.     

Bioactivities of kernel extracts of 18 strains of maize (Zea mays)

Abstract: Aqueous and ethanolic extracts of maize kernels from 18 varieties/strains were prepared for the evaluation of inhibitory activities toward α-glucosidase and scavenging activities toward nitric oxide (NO•) and superoxide (•O₂⁻). All ethanolic extracts of maize strains tested inhibited yeast (Saccharomyces cerevisiae) α-glucosidase with the highest potency (49% to 54%) found for 2 purple and a yellow strains. However, inhibitory effects of maize extracts on rat intestinal α-glucosidase were as a whole about 10% as effective as with the yeast enzyme. Maize extracts were capable of scavenging NO• at the level of 0.25 mg/mL to extents ranging from 24% to 50% and 26% to 57%, respectively, for aqueous and ethanolic extracts. All tested aqueous extracts were also capable of scavenging •O₂⁻, with efficacies ranging from 8% to 38%, at the level of 1.5 mg/mL, whereas almost none of the ethanolic extracts scavenged •O₂⁻, except for one purple strain (approximately 10% effective). The effectiveness in the enzyme inhibition and antioxidant assays did not correlate with total phenolic and/or anthocyanin levels, nor with the nature of pigmentation among the maize strains evaluated. Practical Application : A diversity of pigmented maize strains was evaluated for biological activities related to mitigating oxidative stress and slowing down glucose absorption from the diet. Certain strains tended to be more abundant in these biological activities and have potential to be used in dietary regimes that are designed to promote human health.     

Assessment of the antioxidative potential of dietary supplementation with α-tocopherol in low-nitrite salami-type sausages

The objective of this study was to determine the effects of dietary α-tocopherol supplementation to pigs on the lipid stability of nitrite-reduced salami-type sausages (custom-produced for this study). Fourteen crossbred pigs were divided into two equal groups. The control group (CG) was fed a basal diet containing 34ppm α-tocopherol, and the experimental group (EG), a diet supplemented with 364ppm α-tocopherol, both for 35 days. After slaughter, salami-type sausages were produced with the addition of different nitrite levels in the curing salt (100, 50, 25 and 0ppm), ripened for four weeks, sliced, and packaged under a protective atmosphere (80 parts N(2) to 20 parts CO(2)). The packed products were stored for eight weeks under simulation of the usual conditions in self-service shelves with permanent lighting (9°C, 200 lux). The sausages were regularly assessed for their oxidative status (thiobarbituric acid-reactive substances, fatty acid pattern) and the amount of α-tocopherol, and by sensory evaluation. In the EG sausages, α-tocopherol concentrations were twice as high and TBARS were lower than in the CG sausages. The α-tocopherol in EG prevented the loss of unsaturated fatty acids during storage. Sausages of EG containing 100ppm nitrite had the longest sensory stability. However, the biochemical parameters did not indicate that nitrite had any effect on the antioxidative potential of the sausages.     

Investigation of the effect of branched structure on the performances of the copolymers synthesized from ethylene and α-olefin with rac-Et(Ind)2ZrCl2/MMAO catalyst system

The branched copolymers prepared from ethylene and α-olefins using rac-Et(Ind)₂ZrCl₂/MMAO catalyst system were studied. Both the absolute molecular weight and the molecular size (radius of gyration, R_g) of the polymers eluting from gel permeation chromatography (GPC) columns were obtained simultaneously via a high temperature GPC coupled with a two-angle laser light scattering (TALLS) detector. The branched structures and performances of the copolymers display approximate molecular weight and molecular sizes were investigated. Wide angle X-ray diffraction analyses indicate that 16-carbon side branch could co-crystallize effectively with backbone chain at low α-olefin incorporation. The melt behaviors of the copolymers were studied by dynamic rheological measurements. Both branch length and comonomer content affect considerably the loss modulus, storage modulus and complex viscosity of the copolymers. The relationship between the dynamic-mechanical behavior and the comonomer content of the copolymers was also examined by dynamic-mechanical experiments. It is found that the intensity of β-transition raise with comonomer content increasing.     

4-(吡啶-2-甲氧基)-5,6,7,8-四氢萘酚-1-胺的合成

以α-萘酚为起始原料,经过5步反应得到4-(吡啶-2-甲氧基)-5,6,7,8-四氢萘酚-1-胺,总收率为27%。该制备工艺原料易得、反应条件温和、后处理简单,适合实验室的小规模制备。     

17-AAG对HeLa细胞和V79细胞放射敏感性的影响

为研究17-AAG对肿瘤细胞和正常细胞辐射敏感性的影响,以人宫颈癌Hela细胞和中国仓鼠肺成纤维细胞V79为研究对象,用克隆形成实验观察细胞的存活率。结果表明,17-AAG能明显降低受X射线照射的Hela细胞的存活率,而对V79细胞的存活率则无明显影响,表明17-AAG对HeLa细胞具有放射增敏作用,对V79细胞无放射敏感影响。     

重组人干扰素βSer~(17)的制备及其对SARS病毒的抑制作用

目的制备重组人干扰素-βSer17(rhIFN-βSer17),并检测其对SARS病毒的抑制作用。方法通过发酵收集菌体,经高压匀浆破碎收集包涵体、有机溶剂抽提、酸沉淀、柱层析纯化重组蛋白,通过细胞病变抑制法检测其对SARS病毒的抑制作用。结果研制的rhIFN-βSer17的纯度超过95%,比活性超过2.0×107IU/mg蛋白,在体外对SARS病毒具有明显的抑制作用。结论已成功制备rhIFN-βSer17,并在体外对SARS病毒具有抑制作用。