测井资料高分辨率层序地层分析

高分辨率层序地层学克服了岩性地层对比穿时的缺点.高分辨率层序地层对比是等时的高精度的地层对比,它满足了勘探开发中地层对比等时性和高分辨率的要求.测井资料的纵向分辨率较高,是高分辨率层序地层研究的重要资料.提出了利用测井相分析技术,从测井资料上识别出单井岩性和沉积微相剖面,结合测井曲线特征,划分单井基准面旋回,并进行连井地层等时对比,这为定量地进行高分辨率层序地层分析提供了一种方法.通过研究区实际资料的处理,验证了该方法的有效性.     

二次型时频分析技术的开发与应用

基于二次型的时频分析是高分辨率时频分析技术的有效方法之一，该项技术能够有效反映时变信号的频谱特性，具有分辨率高、能量集中和跟踪瞬时频率的特点。从时变信号的特点出发，阐述了二次型时频分析技术的基本方法原理，采用正演模型分析验证了该方法的有效性，并在实际地震资料中应用于识别物源方向、划分沉积相带、预测储层结构以及相对厚度等诸多方面，均取得了较好的效果。     

Hydro-economic Modeling in River Basin Management: Implications and Applications for the European Water Framework Directive

Economic ideas and processes are becoming increasingly integrated with more traditional engineering and hydrologic models of water management problems. Combining economic management concepts and performance indicators with an engineering-level of understanding of a hydrologic system can provide results and insights more directly relevant for water management decisions and policies. When such models are developed and used with involvement of stakeholders, they can become a basis for shared understanding of water problems as a foundation for negotiated management and policy solutions. When implemented with optimization software, integrated hydro-economic models also can suggest promising innovative solutions for policy-makers to consider. Their applications to river basin management problems are reviewed. Economic and integrated economic-engineering-hydrologic modeling is then discussed in the context of the evolving European Water Framework Directive. Relevant items are cost recovery and water pricing, cost-effectiveness of water management measures, and public participation in decision processes.     

Physical degradation of genomic DNA of soybean flours does not impair relative quantification of its transgenic content

In this paper, four different physical treatments (microwaves, heating by conduction, sonication and pressure autoclaving) were performed to degrade a pure DNA extract, and their influence on GMO quantification was studied. The aim was to check the hypothesis that processing of agrofood products results in a similar degradation rate for both the transgenic target and the specific target. Indeed we could observe that even if the used physical treatments could lead to a significant increase of C _t values for both transgenic and specific targets, the resulting ΔC _t remained stable. So, the main conclusion of the study is that the aforementioned hypothesis seems valid and thus a physical degradation of DNA will not affect the relative quantitation of the GMO content, provided that both the specific and transgenic targets have very similar size. A second important issue of the experiments performed was that DNA is a very robust molecule as it is extremely difficult to reach a mean size below 100 bp. The study also gives evidence of the importance of using small targets.     

Split and collectorless flotation to medium coking coal fines for multi-product zero waste concept

The medium coking coal fines of − 0.5 mm from Jharia coal field were taken for this investigation. The release analysis of the composite coal reveals that yield is very low at 10.0% ash, about 25% at 14% ash and 50% at 17% ash level. The low yield is caused by the presence of high ash finer fraction. The size-wise ash analysis of − 0.5 mm coal indicated that − 0.5 + 0.15 mm fraction contains less ash than − 0.15 mm fraction. Thus, the composite feed was split into − 0.5 + 0.15 mm and − 0.15 mm fractions and subjected to flotation separately. The low ash bearing fraction (− 0.5 + 0.15 mm) was subjected to two stages collectorless flotation to achieve the concentrate with 10% ash. The cleaner concentrate (18.9%) with 10% ash was recovered which has an application in metallurgical industries. The concentrate of 30.2% yield with 12.5% ash could be achieved in one stage collectorless flotation which is suitable for use in coke making as sweetener. As the − 0.15 mm fraction contains relatively high ash, collector aided flotation using sodium silicate was performed to get a concentrate of 23.6% yield with about 17% ash. The blending of this product with cleaner tail obtained from − 0.5 + 0.15 mm produces about 35.0% yield with 17% ash and that can be utilized for coke making. The reject from the two fractions can be used for conventional thermal power plant or cement industries using a 23.5% ash after one stage collector aided flotation and the final tailings produced content ash of 61.6% can be used for fluidization combustion bed (FBC). This eventually leads to complete utilization of coal.     

Halogen-induced charge transfer polymerization of pyrrole in aqueous media

Simultaneous polymerization and doping of pyrrole have been carried out in the presence of a halogenic electron acceptor, bromine (Br₂) or iodine (I₂), in aqueous dispersion or in a two-phase solvent system. The morphology of the polypyrrole (PPY) so produced is granular and porous. The electrical conductivity of the PPY-I₂ charge transfer (CT) complex is of the order of 10¹ ohm⁻¹ cm⁻¹ while that of the PPY-Br₂ complex is about one order of magnitude less. Both complexes are stable in the atmosphere. The physicochemical properties of the PPY-I₂ and PPY-Br₂ CT complexes prepared under various experimental conditions are examined in detail.     

发育有切割井眼结构面的井壁稳定性评价研究

切割井眼结构面的存在弱化了井壁的稳定性，常规完整井眼的井壁稳定分析理论已不能完全适用于受结构面切割的井眼。基于摩尔-库仑理论，在分析结构面应力状态的基础上，给出了井眼受结构面切割的井壁稳定性评价方法，并研究分析了结构面产状对井壁失稳的影响。结果表明，井壁的稳定性与原地应力状态、结构面产状以及结构面的力学性质有关，在其它因素一定的情况下，结构面的走向与倾角对井壁的稳定与失稳方位有着较大的影响。因此，准确评价结构面产出状态是提高井壁稳定性评价精度的前提。     

β-hydroxyacyl-coa-dehydrogenase (HADH) activity of unfrozen and frozen—thawed frog (Rana esculenta) legs

The β-hydroxyacyl-CoA-dehydrogenase (HADH) activity of unfrozen and thawed frog legs was investigated. The enzyme was extracted by either immersing frog legs in phosphate buffer 0.1 M, pH 6.0 at 25°C for 15 min or pressing them between trichinoscopy glasses. The enzyme activity was assayed using acetoacetyl-CoA as substrate and measured spectrophotometrically at 340 nm. It was possible by both extraction methods to distinguish between thawed and unfrozen samples although when the juice was obtained by pressing the HADH activity of the dilution was ～ 1.5 times higher than that obtained by immersion. The HADH activity was significantly higher (P≤0·001) in frozen-thawed frogs than in unfrozen legs because during freezing there is a release of HADH. No significative differences were found in the HADH activity in samples frozen in the temperature range -10 to -196°C. HADH activity was not affected by the storage time in crushed ice up to 6 days.     

Yeast exo-β-glucanases can be used as efficient and readily detectable reporter genes in Saccharomyces cerevisiae

Yeast exo-1,3-β-glucanases are secretable proteins whose function is basically trophic and may also be involved in cell wall glucan hydrolytic processes. Since fluorescein di(β-D -glucopyranoside) is a fluorogenic substrate detectable and quantifiable by flow cytometry, it was used for testing the ability of the EXG1 gene product of Saccharomyces cerevisiae and its homologous gene in Candida albicans to function as reporter genes. These open reading frames were coupled to different promoters in multicopy plasmids, and exoglucanase activity quantified at flow cytometry. Exoglucanases were found to be useful tools for the study of promoter regions in S. cerevisiae. This technique has the advantage over other reporter gene systems—such as β-galactosidase fusions—that it does not require permeabilization of yeast cells and therefore it allows the recovery of viable cells—by sorting—after flow cytometry analysis.     

Rates of Crystallization of Dried Lactose-sucrose Mixtures

The isothermal crystallization kinetics of glassy lactose/sucrose mixtures were studied at several storage temperatures (close to T_g and T_m). The kinetic parameters implicit in the Avrami equation were determined. Activation energies for transport (E_D) and surface nucleation (W*) were also found and correlated to the molar composition of the lactose/sucrose mixtures. A monotonic increase in the half crystallization time (t_1/z), Avrami index (n), % crystallization per day, activation energy for transport (E_D) and surface nucleation energy (W*) and a decrease in the crystallization velocity constants (K) were related to the increase in the lactose content of lactose/sucrose mixtures.