One‐step production of 2,3‐butanediol from starch by secretory over‐expression of amylase in Klebsiella pneumoniae

BACKGROUND: 2,3‐Butanediol (2,3‐BD) is a valuable chemical that can be biosynthesized from many kinds of substrates. For commercial biological production of 2,3‐BD, it is desirable to use cheap substrate without pretreatment, such as starch. However, there have been few reports on the production of 2,3‐BD directly from starch. RESULTS: In this work, gene malS coding for α‐amylase (EC 3.2.1.1) precursor was inserted into plasmid pUC18K, and secretory over‐expression of α‐amylase was achieved by engineered Klebsiella pneumoniae. The extracellular recombinant amylase accelerated the hydrolyzation of starch, and one‐step production of 2,3‐BD from starch was carried out by engineered K. pneumoniae. A 2,3‐BD concentration of 3.8 g L^?1 and yield of 0.19 g 2,3‐BD g^?1 starch were obtained after 24 h fermentation. CONCLUSION: The one‐step production of 2,3‐BD from starch was achieved by secretory over‐expression of amylase in K. pneumoniae. This would simplify the process and reduce the production cost considerably by enabling use of starch with minimal pretreatment. Copyright © 2008 Society of Chemical Industry     

科研项目管理信息系统的设计

介绍了用自顶向下的结构化方法进行科研项目管理信息系统设计的过程。该系统的应用节省了大量人力、物力和财力,提高了企业科研项目管理水平。     

人类HLA-DRB1基因型与民族和人群的神经网络无监督模式分类

HLA-DRB1等位基因主要参与人类的抗原免疫功能,对其进行比较研究,有助于追溯人类的进化迁移史,开发类群特异性药物.本研究以Matlab为平台,用自组织竞争网络(Self-Organizing Competitive Neural Network),对世界54个民族和人群、14个HLA-DRB1等位基因,进行了无监督模式分类.结果表明,各民族之间存在差异性,同民族的各人群之间有相似性.西伯利亚各人群,澳洲各土著人群,黑人各人群,南美印第安各人群,犹太族各人群,日本各人群,及欧美白人各人群有相对独立性:南美印第安人与西伯利亚人有高度相似性;中国民族在南方人群和北方人群间存在较大差异;中国汉族中,广东汉族地位特殊,与少数民族如拉祜族、瑶族关系密切.     

Periodic surface modeling for computer aided nano design

Current solid and surface modeling methods based on Euclidean geometry in traditional computer aided design are not efficient in constructing a large number of atoms and particles. In this paper, we propose a periodic surface model for computer aided nano design such that geometry of atoms and molecules can be constructed parametrically. At the molecular scale, periodicity of the model allows thousands of particles to be built efficiently. At the meso scale, inherent porosity of the model represents natural morphology of polymer and macromolecule. Surface and volume operations are defined to support crystal and molecular model creation with loci and foci periodic surfaces. The ultimate goal is to enable computer assisted material and system design at atomic, molecular, and meso scales.     

错金工艺及对其运用的探讨

为了继承和发扬错金工艺，从其定义出发，探讨了该工艺在中国古代及国外的发展与应用以及笔者对该工艺的实验研究。针对在错金工艺实践过程中出现的问题，在查阅了相关的资料后，对传统错金工艺的实现方法提出了质疑。     

Comparative study of the elimination of toluene vapours in twin biotrickling filters using two microorganisms Bacillus cereus S1 and S2

BACKGROUND: To investigate the microbial degradation performance of organic pollutants in the atmosphere using a biotrickling filter, two microorganism strains, Bacillus cereus S1 and Bacillus cereus S2, were selected, identified and inoculated into a twin biotrickling filter for comparison. RESULTS: Both strains showed good performance towards the degradation of model organic pollutants when gas flow rates ranged from 100 to 600 L h⁻¹. For S1, the total maximum removal efficiency (RE) of toluene was maintained nearly 100% not only at gas flow rates of 100 L h⁻¹ corresponding to empty bed residence time (EBRT) 199.44 s, but also at gas flow rates of 200 L h⁻¹ (EBRT = 99.72 s) and 300 L h⁻¹ (EBRT = 66.48 s). However, S2 had a much lower degradation capability; near 100% removal efficiency was obtained only at the gas flow rate of 100 L h⁻¹ although both bacteria belong to the same Bacillus cereus. With further increase in gas flow rate, the total REs for both S1 and S2 decreased slightly at first and then dropped sharply to 46% and 35%, respectively, at an EBRT of 33.24 s, corresponding to a gas flow rate of 600 L h⁻¹. Starvation for between 2 and 10 days resulted in the re‐acclimation times of both strains ranging between 1.0 and 15.5 h. CONCLUSION: Strain S1 would be a better choice for inoculation into a biotrickling filter than strain S2, because of its much higher toluene removal capacity and rapid recovery to full performance. Copyright © 2008 Society of Chemical Industry     

轮式工程机械轮边减速器优化模型与方法

论述了重构轮式工程机械轮边减速器优化设计数学模型的必要性,给出了精确化的目标函数,全面化和准确化的设计变量、基于并行设计的约束条件;介绍了对优化解适用性评价的方法和手段。巧妙地解决了优化解中离散变量的圆整问题,提供了基于MATLAB的求解反渐开线函数的M文件。     

正交实验优选盐生藻多糖的提取工艺

以盐生藻为原料,盐生藻多糖得率为指标,运用稀酸、稀碱和蒸馏水为提取剂提取盐生藻多糖,得出合适的提取剂为稀酸.进一步的正交实验表明,盐生藻多糖的最适提取工艺为:提取75min,pH=4,固液比为1∶70,温度为95℃.     

水上地震折射互换法误差分析

在理论上，用源检互换的方法进行折射地震勘探是没有误差的，在某些特殊条件下无法铺设检波器而又必须进行折射地震勘探时它也是唯一的选择，然而，在实际工作中，由于各种因素的影响，其误差是不可忽略的。为此，本文从地震折射运动学的角度对水上地震折射互换法因过河钢丝绳的重量、水的流速和流向、炮点深度等客观因素造成的解释误差进行了分析，揭示了水上地震折射互换法应注意的问题。这对水上地震折射互换法的资料解释进行误差校正提供了理论基础。     

一种基于像素自适应仲裁的OSD分层混合结构设计

数据混合是OSD分层显示的关键技术之一.本文基于OSD的分层结构及数据组织结构特点,提出一种OSD分层显示的单混合结构,该结构采用基于像素自适应仲裁的策略,按照层优先级次序,对多个OSD图层信号实时地切换显示.FPGA验证结果表明,该结构在满足OSD分层混合显示、实现菜单选择鼠标控制等高级人机对话功能的前提下,其硬件资源开销比多混合结构节省约33%以上,且具有良好的层次可扩展性和操作灵活性.