高坝泄洪诱发场地振动响应分析及传播规律

为研究高坝泄洪诱发场地振动的问题,提出一种基于黏弹性人工边界的有限元数值模拟和水力学模型试验相结合的方法计算场地振动响应。以国内某工程为例,计算不同工况下场地振动响应,分析泄洪量和泄洪调度方式对场地振动的影响,揭示不同岩体条件下振动波的传播规律。结果表明:该工程各种泄洪方式都不会对周边居民的生活造成影响;泄洪量越大,下游场地振动响应越大,当3个溢洪洞泄洪条件一致时,采用2号洞泄洪最不利;同等泄洪条件下,采用4洞均分泄洪的振动响应小于按设计开度泄洪的振动响应;模型范围内放大振动波的主因是断层左右岩体性质的差异,断层的存在进一步加大了放大效应。     

避暑山庄的选址

造园首在选址。选址得当,可以事半功倍。计成在《园冶》中指出,只有“相地合宜”,才能“构园得体”。地址要好,清洁卫生,有丘有壑,草地和树林相间”。避暑山庄之所以能够在造园史上独树一帜,选址得当是其中一个重要原因。避暑山庄当时所以选择这块地方建造宫苑,除政治上的原因之外,主要是因为它具有优越的自然条件。避暑山庄的园林艺术成就,已得到世人的公认,造园家相地择址的匠心,也会给我们许多有益的启示。     

Single amino acid substitutions can further increase the stability of a thermophilic L-lactate dehydrogenase

Lactate dehydrogenases are of considerable interest as stereospecificcatalysts in the chemical preparation of enantiomerically pure-hydroxyacid synthons. For such applications in synthetic organicchemistry it would be desirable to have enzymes which tolerateelevated temperatures for prolonged reaction times, to increaseproductivity and to extend then applicability to poor substrates.Here, two examples are reported of significant thermostabilizations,induced by sitedirected mutagenesis, of an already thermostableprotein, the L-lactate dehydrogenase (EC 1.1.1.27 [EC] , 35 kDa permonomer subunit) from Bacillus stearothermophilus. Thermal inactivationof this enzyme is accompanied by irreversible unfolding of thenative protein structure. The replacement of Argl71 by Tyr stabilizesthe enzyme against thermal inactivation and unfolding. Thisstabilizing effect appears to be based on improved interactionsbetween the subunits in the core of the active dimeric or tetramericforms of the enzyme. The thermal stability of L-lactate dehydrogenasevariants with an active site Arg residue, either in the 171(wild-type) or in the 102 position, is further increased bysulfate ions. The two stabilizing effects are additive, as foundfor the Argl71Tyr/ Gln1O2Arg double mutant, for which the stabilityof the protein in 100 mM sulfate solution reaches that of L-lactatedehydrogenases from extreme thermophiles. All mutant proteinsretain significant catalytic activity, both in the presenceand absence of stnhilfoing salts, and are viable catalysts inpreparative scale reactions.     

装饰设计专业《计算机辅助设计》辅助教学网站的建设研究

装饰艺术设计专业《计算机辅助设计》课程的教学,既要能学会熟练操作各类艺术设计软件,又要有装饰制图知识、艺术设计能力和独创性思维能力。而各类辅助设计软件彼此之间缺少必要的联系和连贯性,学生很难系统掌握。因此建设本课程的辅助教学网站很有必要,使传统课堂讲授和网络教学相结合。本文同时还从网站建设思路和建设方案等方面来探讨本课程的网站辅助教学方法。     

Site occupations and ordering behaviour of O phase in Ti2AlNb—based intermetallics

1　ＩＮＴＲＯＤＵＣＴＩＯＮＴｈｅｏｒｔｈｏｒｈｏｍｂｉｃａｌｌｏｙｓａｒｅｏｆｇｒｅａｔｔｅｃｈ ｎｏｌｏｇ ｉｃａｌｉｎｔｅｒｅｓｔｉｎｈｉｇｈｔｅｍｐｅｒａｔｕｒｅａｐｐｌｉｃａｔｉｏｎｓｓｉｎｃｅｔｈｅｙｈａｖｅａｇｏｏｄｂａｌａｎｃｅａｍｏｎｇｓｔ     

智能化磁控谐振高压试验系统研究

目前电网智能化水平的提升对高压试验的智能化和试验效率也有了更高的需求,为解决现有的高压试验电源在高压交流绝缘试验中存在的不足,提升高压试验的效率,提出了一种智能化磁控谐振高压试验系统。该试验系统集升压、补偿和调谐于一体,具有升压调节平滑连续,结构紧凑等优势,可用于工频耐压试验。阐述了该系统的结构组成与基本原理,并搭建了该试验系统仿真模型,通过研究空载和负载运行特性验证了该系统原理的正确性。     

The GAUGAA Motif Is Responsible for the Binding between circSMARCA5 and SRSF1 and Related Downstream Effects on Glioblastoma Multiforme Cell Migration and Angiogenic Potential

Circular RNAs (circRNAs) are a large class of RNAs with regulatory functions within cells. We recently showed that circSMARCA5 is a tumor suppressor in glioblastoma multiforme (GBM) and acts as a decoy for Serine and Arginine Rich Splicing Factor 1 (SRSF1) through six predicted binding sites (BSs). Here we characterized RNA motifs functionally involved in the interaction between circSMARCA5 and SRSF1. Three different circSMARCA5 molecules (Mut1, Mut2, Mut3), each mutated in two predicted SRSF1 BSs at once, were obtained through PCR-based replacement of wild-type (WT) BS sequences and cloned in three independent pcDNA3 vectors. Mut1 significantly decreased its capability to interact with SRSF1 as compared to WT, based on the RNA immunoprecipitation assay. In silico analysis through the “Find Individual Motif Occurrences” (FIMO) algorithm showed GAUGAA as an experimentally validated SRSF1 binding motif significantly overrepresented within both predicted SRSF1 BSs mutated in Mut1 (q-value = 0.0011). U87MG and CAS-1, transfected with Mut1, significantly increased their migration with respect to controls transfected with WT, as revealed by the cell exclusion zone assay. Immortalized human brain microvascular endothelial cells (IM-HBMEC) exposed to conditioned medium (CM) harvested from U87MG and CAS-1 transfected with Mut1 significantly sprouted more than those treated with CM harvested from U87MG and CAS-1 transfected with WT, as shown by the tube formation assay. qRT-PCR showed that the intracellular pro- to anti-angiogenic Vascular Endothelial Growth Factor A (VEGFA) mRNA isoform ratio and the amount of total VEGFA mRNA secreted in CM significantly increased in Mut1-transfected CAS-1 as compared to controls transfected with WT. Our data suggest that GAUGAA is the RNA motif responsible for the interaction between circSMARCA5 and SRSF1 as well as for the circSMARCA5-mediated control of GBM cell migration and angiogenic potential.     

基于水质模拟的水质在线监测站点布设研究

随着水质监测连续化的需求增加,河流水质在线监测站布设成为不可忽视的问题。本文采用水文模拟模型HSPF对台湾淡水河流域进行水量水质联合模拟。水文模拟以9个测站比对,纳什效率系数多数大于0.7;相关系数r皆大于0.7。水质模拟以27个测站比对,生化需氧量、氨氮、悬浮固体、溶氧的修正平均百分误差多数小于25%。基于水质变化率和水质超标率提出水质敏感河段概念,并以此确定为补充布设水质在线监测站点的优先河段。研究指出,淡水河流域水质在线监测站目前仍缺乏且位置不尽合理,应优先补充三峡河的三峡大桥、景美溪的宝桥、基隆河的江北桥水质在线监测站。     

用攀枝花钛矿和云南钛矿冶炼钛渣工业试验研究

通过磁性测量和X-射线衍射研究了Gd2(Co，Al)17化合物的结构和磁晶各向异性。当x≤4时，样品具有菱方Th2znl7型结构，x=5时，具有六方CaCu5型结构。随着Al浓度增加，晶格常数和单胞体积单调增大，而居里温度和自发磁化强度近似线性地减小；在x≥l时，观察到Co亚点阵的磁晶各向异性变号，室温易磁化方向由易面转变为易轴，且易轴各向异性场和各向异性常数随Al原子浓度增加出现极大值；基于补偿温度(65K)下自由粉末颗粒的磁化曲线，导出了亚点阵间的分子场系数和Gd-co交换耦合常数，结果表明，Al原子对Gd-Co交换耦合作用的影响较小。根据Al原子在Co亚点阵4种晶位的择优占位，分析了磁晶各向异性的演变。     

除草剂对植物和动物共有靶标的毒性机制

除草剂对哺乳动物和植物的毒性是通过对靶标位点的作用而产生的,目前已发现了近20种不同的除草剂作用机制,它们的靶标位点有些是植物所特有的,有些是哺乳动物和植物以及微生物所共有的。就这些除草剂的作用机制进行了系统的阐述,比较了它们对植物和哺乳动物的相对活性,以及如何确定它们的毒性。同时,除靶标位点外,除草剂的吸收、传导、代谢和降解也是影响毒性的重要因素,对这些因素的研究也有助于提高对非靶标生物的安全性。