立地对油茶籽出油率及油茶籽油品质的影响研究

为了探明立地对油茶籽出油率及油茶籽油品质的影响,以9个立地的低温压榨油茶籽油为样品,测定油茶籽出油率及油茶籽油的理化性质、感官品质、脂肪酸组成、功能性成分含量及氧化诱导时间,分析坡向、坡位因子对油茶籽出油率及油茶籽油品质的影响。结果表明:立地对油茶籽出油率及油茶籽油的过氧化值、碘值、皂化值、感官品质、功能性成分含量及氧化诱导时间均具有明显影响,对油茶籽油的酸价、脂肪酸组成无明显影响。说明立地是影响油茶籽出油率及油茶籽油品质的重要因素。     

The Role of Heparan Sulfate in CCL26-Induced Eosinophil Chemotaxis

Proinflammatory chemokine ligand 26 (CCL26, eotaxin-3) mediates transendothelial cell migration of eosinophils by binding and activating the G-protein-coupled (GPC) chemokine receptor 3 on the surface of eosinophilic cells. Here we have investigated the role of glycosaminoglycans (GAGs) as potential co-receptors in the process of CCL26-induced eosinophil chemotaxis. For this purpose, we have first identified the GAG-binding site of CCL26 by a site-directed mutagenesis approach in the form of an alanine screening. A panel of GAG-binding-deficient mutants has been designed, generated, and analyzed with respect to their binding affinities to heparan sulphate (HS) by isothermal fluorescence titration studies. This showed that basic amino acids in the α-helical part of CCL26 are strongly involved in GAG-binding. In chemotaxis experiments, we found that decreased GAG-binding affinity correlated with decreased chemotactic activity, which indicates an involvement of GAGs in eosinophil migration. This was further proven by the negative impact of heparinase III treatment and, independently, by the incubation of eosinophils with an anti heparan sulfate antibody. We finally investigated eosinophils’ proteoglycan (PG) expression patterns by real-time PCR, which revealed the highest expression level for serglycin. Including an anti-serglycin antibody in CCL26-induced eosinophil migration experiments reduced the chemotaxis of these immune cells, thereby proving the dependence of eosinophil mobilization on the proteoglycan serglycin.     

Generation and Identification of the Number of Copies of Exogenous Genes and the T-DNA Insertion Site in SCN-Resistance Transformation Event ZHs1-2

Soybean cyst nematode (SCN, Heterodera glycines Ichinohe) causes an estimated economic loss of about USD 3 billion each year in soybean (Glycine max L.) production worldwide. Overexpression of resistance genes against SCN provides a powerful approach to develop SCN resistance cultivars in soybean. The clarification of molecular characterization in transformation events is a prerequisite for ecological risk assessment, food safety, and commercial release of genetically modified crops. Here, we generated transgenic events harboring the BCN (beet cyst nematode) resistance Hs1^pro−1 gene using the Agrobacterium-mediated method in soybean, evaluated their resistance to SCN infection, and clarified the molecular characterization of one of the transformation events. Five independent and stable inheritable transformation events were generated by an Agrobacterium-mediated transformation method. SCN resistance tests showed the average number of developed females per plant and female index (FI) in T4 ZHs1-1, ZHs1-2, ZHs1-3, ZHs1-4, and ZHs1-5 transformation events were significantly lower than that in the nontransgenic control. Among these, the ZHs1-2 transformation event had the lowest number of developed females per plant and FI. Southern hybridization showed the exogenous target Hs1^pro−1 gene was inserted in one copy and the Bar gene was inserted two copies in the ZHs1-2 transformation event. The exogenous T-DNA fragment was integrated in the reverse position of Chr02: 5351566–5231578 (mainly the Bar gene expression cassette) and in the forward position of Chr03: 17083358–17083400 (intact T-DNA, including Hs1^pro−1 and Bar gene expression cassette) using a whole genome sequencing method (WGS). The results of WGS method and Southern hybridization were consistent. All the functional elements of exogenous T-DNA fragments were verified by PCR using specific primer pairs in the T5 and T6 ZHs1-2 transformation events. These results demonstrated that the overexpression of Hs1^pro−1 gene enhanced SCN resistance, and provide an important reference for the biosafety assessment and the labeling detection in transformation event ZHs1-2.     

广西容县古代田辽岭冶铜遗址初步研究

田辽岭冶铜遗址位于广西容县容西乡西山村,面积约0.12 km2,考古年代属汉唐时期。对取自该遗址的18个炉渣运用扫描电子显微镜及能谱分析（SEM-EDS）方法进行成分分析;对其中2个炉渣样品采用变形法进行熔点测定。检测结果表明,该遗址采炼铜铅多金属矿石,使用“氧化矿—铜”工艺冶炼红铜。该遗址冶炼温度高,铜渣分离较好,该遗址冶炼技术比较成熟。     

珠江虎门河口洪季潮流近底边界层水流结构研究

研究河口近底边界层水动力结构对进一步研究河口地区水流能量耗散和物质输运具有重要意义,2006年7月26～28日在珠江虎门口采用ADV、ADP及OBS和CDT组合置底支架观测系统对近底边界层水流、泥沙和温盐度进行了水下定点观测,本文通过分析流速观测数据,对该潮汐河口近底边界层的水流结构、阻力特性和紊动尺度进行研究,结果表明除憩流附近外,近底边界层内时均流速分布均可采用对数剖面,摩阻流速值与时均流速基本成正比,平均摩阻流速为0.014m/s,粗糙长度为0.09mm,切应力梯度为0.426N/m~2·m;脉动流速统计分析结果符合典型潮汐河口底边界层的紊动特点,距床面0.15m处,落急时刻宏尺度紊动的时间尺度和长度尺度分别为3s和0.06m;垂向脉动分速以及瞬时雷诺应力自相关分析表明转流附近时段的湍流主要以低频大尺度紊动为主.测量分析结果可为下一步研究近底边界层泥沙运动提供依据,并为数值模拟计算提供水动力分析条件和特征参数.     

互动式基建现场管控中心的建设及应用

针对当前电网建设项目多、分布广、管理人员少的现状,利用3G网络建成省内首家互动式基建现场管控中心,实现了实时监控、手机监控、远程数码照片采集、远程安全检查、E信整改、实时技术会诊等一系列功能。管控中心建成后,可定期进行远程安全检查、远程数码照片采集,在促进施工规范化、保障施工安全方面取得了显著效果。     

基于综合敏感性分析的时序分布式电源规划

随着配电网中分布式电源渗透率的逐渐提高,对电网电压和网损的影响变得明显。这种影响和分布式电源规划的位置和容量密切相关。提出的时序分布式电源规划的方法:首先利用节点电压和网损综合敏感性分析,筛选出分布式电源的安装位置节点;然后利用优化方法对分布式电源容量优化。其中容量优化方法:以减少网络损耗和提高电压水平两个技术指标建立目标函数,利用机会约束规划方法构建约束条件;并使用蒙特卡洛模拟的方法对分布式电源和负荷时序模型进行随机潮流计算;最后利用精英策略的遗传算法求得分布式电源的最优配置容量。算例仿真结果验证了该规划方法的有效性。     

环形配电网的分频调节阻性有源电力滤波器位置策略

通过理论分析环形配电网的谐波放大原理,对比安装在线路中点且阻抗匹配的阻性有源电力滤波器(RAPF)和对称安装在不同位置的分频调节RAPF的抑制效果,提出了一种适用于环形配电网的分频调节RAPF的位置策略。若线路长度小于谐波波长的1/2,靠近线路中点的对称位置为最优安装位置;若线路长度大于谐波波长的1/2,距谐波源1/4波长的对称位置为最优安装位置。与安装在线路中点且阻抗匹配的RAPF相比,安装在最优位置的分频调节RAPF,当其电导增益≥匹配的电导时,均能有效地衰减谐波,获得更好的抑制效果;且电导增益越大,谐波抑制效果越好。同时,该方案对线路参数的改变具有更好的适应性。仿真和实验结果验证了该方案的有效性。     

航空物探试验场地面地球物理场特征分析

航空地球物理探测技术是地质和矿产勘查的重要手段,其探测能力主要取决于测量系统及测量方法的有效性,而测量系统及测量方法有效性的提高,又需要专业的检验平台(航空物探试验场)。因此,在我国建立专业的航空物探试验场十分必要。本文搜集了我国某航空物探试验场的地质地球物理资料,在试验场设计并完成了重力、电阻率测深、瞬变电磁工作。通过对实测资料的处理和研究,将试验场划分为三个大的异常区,确定了试验场内的两个较大的断裂和一个次级断裂。本文在国内首次以专项工作的方式为航空重力及电磁法测量提供了高精度的参考数据,从而为国内航空地球物理测量系统检验和方法研究提供了综合平台。并且通过多种物探方法建立标准场的实践认识到,通过有效的选址,在充分利用已有的地质、地球物理(地面、航空)及测绘等资料的基础上,可以完善建成综合试验场,为航空物探飞行测量提供可靠的高精度对比资料。