一种基于显微多光谱宫颈细胞图像自动分割方法

介绍了一种基于多光谱宫颈显微图像自动分割方法。细胞涂片每一个局部区域经过显微镜放大后再通过电调谐光器件分别取出不同光谱波段的图像，经过CCD送入计算机后得到该视场分析所需的多光谱图像。从所获得的多光谱图像中选择一定的波段图像对数变换相除、多阈值分割和形态学操作，最后获得宫颈细胞的胞浆和胞核覆盖层。本文首次将多光谱分析应用到宫颈显微图像分割中，具有分割准确、分割速度快、能够去除胞浆重叠和受外界干扰少的特点。     

Passport 15000交换机在福建电力通信网中的应用

北电Passport15000交换机作为福建省ATM宽带数据广域通信网中的骨干节点，具有40Gbit／s的大交换容量，支持如ATM、帧中继、IP和话音等多种业务的综合数据交换平台。文中介绍了该设备的技术特点及应用情况，分析了对其实现保护投资的应用前景。     

甲烷制备高纯度氢气的实验研究

对甲烷制备可供质子交换膜燃料电池(PEMFC)用的高纯度氢气进行了实验研究，得出了不同蒸汽／碳比值时甲烷的转化率、不同过量空气系数对制备气体成分的影响、不同压差时氢气在选择性膜中的渗透率以及不同系统压力对氢气反应焓的影响规律．研究结果表明，在蒸汽与甲烷中的碳比例一定时，蒸汽重整反应器内的反应速率和它出口处CO的浓度，随甲烷的流量的增加而提高．提高甲烷蒸汽重整系统的工作温度和压力，可以提高甲烷的转化率，在S／C比值不变的情况下，仅提高温度可提高甲烷的转化率约10％；而将工作压力由0．1MPa提高到0．6MPa，甲烷的转化率提高约21％．甲烷的理论转化率为84．6％，本文实验研究中的最大实际转化率为78．03％，实验研究系统的系统完善度为92．23％，说明该系统还有待完善之处，还可以进一步提高甲烷的转化率．增大PEMFC中选择性膜两侧的压差或提高氢气的纯度，均利于提高氢气混合物的渗透率．该研究为制备符合PEMFC使用条件的氢气提供了可靠依据，同时为综合利用煤层气提供了新的途径。     

流场尺寸对质子交换膜燃料电池性能的影响

流场的形状和尺寸是设计双极板的核心。合理的流场既要确保电极各处均能获得充足的反应剂供应,同时又要保证反应产物水的排出。研究了不同流场尺寸对质子交换膜燃料电池性能的影响。实验结果表明:在一定范围内,随着开孔率的增加,电极与流场本相及接触电阻也逐渐增加;开孔率相近时,这一电阻相差不大。在相同开孔率的情况下,沟槽尺寸越小,电池性能越好。     

Investigation of Structure and Hydriding Properties of La0.6 Nd0.4 Ni4.8 Mn0.2 Cux Alloys

The structure and hydriding performance of La0.6Nd0.4Ni4.8Mn0.2Cux (x = 0 ～ 0.4) alloys were investigated in order to develop suitable materials for metal hydride air conditioner. The effect of Cu addition on the crystal structure,equilibrium pressure, hydrogen capacity and hysteresis as well as hydrogen absorption/desorption kinetics were systematically studied by using the measurement of P-C isotherms, X-ray diffraction and scanning electron microscopy. As the amount of Cu increases, the plateau pressure increases and hydrogen absorption/desorption kinetics is improved, but the effective hydrogen storage capacity decreases. It is shown that variations in the basal plane parameter a can be used as an indication for the plateau pressure changes. With the increase of parameter a, the plateau pressure decreases. For La0.6 Nd0.4Ni4.8Mn0.2Cux(x = 0 ～ 0.4) alloys there is a relationship between the effective hydrogen storage capacities and the ratios of their unit cell parameters c and a. The effective hydrogen storage capacity decreases with increase of a/c.     

Backscattered electron imaging of the undersurface of resin-embedded cells by field-emission scanning electron microscopy

In this study backscattered electron (BSE) imaging was used to display cellular structures stained with heavy metals within an unstained resin by atomic number contrast in successively deeper layers. Balb/c 3T3 fibroblasts were cultured on either 13-mm discs of plastic Thermanox, commercially pure titanium or steel. The cells were fixed, stained and embedded in resin and the disc removed. The resin block containing the cells was sputter coated and examined in a field-emission scanning electron microscope. The technique allowed for the direct visualization of the cell undersurface and immediately overlying areas of cytoplasm through the surrounding embedding resin, with good resolution and contrast to a significant depth of about 2 μm, without the requirement for cutting sections. The fixation protocol was optimized in order to increase heavy metal staining for maximal backscattered electron production. The operation of the microscope was optimized to maximize the number of backscattered electrons produced and to minimize the spot size. BSE images were collected over a wide range of accelerating voltages (keV), from low values to high values to give ‘sections' of information from increasing depths within the sample. At 3–4 keV only structures a very short distance into the material were observed, essentially the areas of cell attachment to the removed substrate. At higher accelerating voltages information on cell morphology, including in particular stress fibres and cell nuclei, where heavy metals were intensely bound became more evident. The technique allowed stepwise ‘sectional’ information to be acquired. The technique should be useful for studies on cell morphology, cycle and adhesion with greater resolution than can be obtained with any light-microscope-based system.     

Vertical sections of cultivated anchorage-dependent cells for electron microscopy

A method is described for electron microscopic preparation of cultivated cells for vertical sections using the test chamber system TCSC-1. The cells are cultivated on a special foil. They can be fixed and embedded directly in the chamber. After polymerization of the resin, the foil can be easily taken off and a second resin layer is poured upon the embedded cells. Then distinct cells can be marked under an inverted light microscope. The bilayer of the resin allows optimal conditions for vertical sections of anchorage-dependent cells.     

Microscopy under pressure--an optical chamber system for fluorescence microscopic analysis of living cells under high hydrostatic pressure

High hydrostatic pressure (HHP) becomes more and more interesting for life science research, since it can be employed to inactivate various cells. To directly monitor "cells under pressure," the development of an optical high-pressure chamber is required. Therefore, an optical pressure chamber that can be used for up to 300 MPa was constructed. This chamber has already been described as a tool for in situ observation of dynamic changes of microscopic structures in bright field as well as phase contrast. In combination with an inverted microscope, we obtained brilliant microscopic color pictures with an optical resolution more than 0.56 microm. Here, we demonstrate the capabilities of the HHP cell, in combination with epifluorescence microscopy. Using a nonadherent human B-cell line (Raji, ATCC CCL 86), stained with the fluorescent dyes propidium iodide, Hoechst 33342, or dihexyloxacarbocyanine iodide, we were able to show that the system is suitable to perform fluorescence microscopic analyses, with pressures up to 300 MPa, with viable mammalian cells.     

Consideration on Effective Diffusivity of Strontium in Granite

Abstract

Diffusive behavior of strontium and certain kinds of divalent cations in Inada granite were studied by a through-diffusion method. In order to examine the effect of sorption onto overall diffusive behavior, two kinds of solutions were used: 0.1M KCl solution and deionized water. The effective diffusion coefficient (D_e ) and rock capacity factor (a) were (2.0–3.6) xl0–¹³ m²/s and less than 0.022 in 0.1M KCl solution and (0.32–1.7) x 10 ^?13m²/s and 1.5–2.4 in deionized water respectively. The D_e , and α in deionized water were much larger than those in 0.1M KCl solution. These results are well explained by taking into account the diffusion of sorbed ion or the surface diffusion. In support of this mechanism, most D_e , values of Sr reported for various rocks are found proportional to the sorptivity ( _ρR_d )-In the case that the sorptivity is low, D_e of Sr depends on porosity like that of nonsorbed iodide. The effective diffusion coefficient of Sr in rocks was well explained by taking into account pore and surface diffusion and was expressed as D_e=2.1 xl0^?10 _? ^1.3+3.5xl0″^?12 _ρR_d . The effective diffusion coefficient of divalent cations in the granite was found proportional to their diffusion coefficients in bulk solution.     

脱碳处理对Pt/WC复合材料催化性能的影响

采用溶胶-凝胶法,以钨酸钠为钨源、酚醛树脂为碳源合成碳化钨前驱体干凝胶,经原位碳化制备纳米碳化钨（WC）粉体,纳米WC粉体中的玻璃碳采用浓硝酸浸渍法脱除。在纳米WC粉料浆中以甲醛（HCHO）作为氯铂酸（H2PtCl6·6H2O）的还原剂,室温合成得到Pt/WC复合催化剂材料;以XRD、SEM、EDS等测试方法对样品的理化特性进行表征,并在酸性介质中采用循环伏安法测试材料的电化学催化活性。结果表明：相较于未经过脱碳处理的WC,经过浓硝酸脱碳处理后的Pt/WC电极催化材料的催化活性更好。经溶胶-凝胶法及原位碳化制备的纳米WC的颗粒径为20-80纳米,其本身具备较好的电催化活性。样品中残留的玻璃碳对WC具有包覆作用,会降低WC的电催化活性。采取浓硝酸脱碳的方式可有效脱除纳米WC粉体中的玻璃碳,发挥WC材料作为电极催化材料的电催化活性。