Molecular Dynamics of CYFIP2 Protein and Its R87C Variant Related to Early Infantile Epileptic Encephalopathy

The CYFIP2 protein (cytoplasmic FMR1-interacting protein 2) is part of the WAVE regulatory complex (WRC). CYFIP2 was recently correlated to neurological disorders by the association of the R87C variant with early infantile epileptic encephalopathy (EIEE) patients. In this set of syndromes, the epileptic spasms and seizures since early childhood lead to impaired neurological development in children. Inside the WRC, the variant residue is at the CYFIP2 and WAVE1 protein interface. Thus, the hypothesis is that the R87C modification weakens this interaction, allowing the WRC complex’s constant activation. This work aimed to investigate the impacts of the mutation on the structure of the WRC complex through molecular dynamics simulation. For that, we constructed WRC models containing WAVE1-NCKAP1 proteins complexed with WT or R87C CYFIP2. Our simulations showed a flexibilization of the loop comprising residues 80–110 due to the loss of contacts between internal residues in the R87C CYFIP2 as well as the key role of residues R/C87, E624, and E689 in structural modification. These data could explain the mechanism by which the mutation impairs the stability and proper regulation of the WRC.     

基于数据仓库的宏观经济监测预警系统的设计

宏观经济监测预警是对宏观经济进行总体的、综合的、全面的、系统的分析与判断,是运用理论分析方法、经验分析方法、数理统计方法等对宏观经济循环波动这一特定现象进行一整套经济监测、经济评价的体系。介绍了宏观经济监测预警系统的实现环境、预警指标体系的设计、数据仓库的设计以及系统的总体设计。并在此基础上,简要地介绍相关子系统的功能。     

Genetic LGALS1 Variants Are Associated with Heterogeneity in Galectin-1 Serum Levels in Patients with Early Arthritis

Galectin 1 (Gal1) exerts immunomodulatory effects leading to therapeutic effects in autoimmune animal models. Patients with rheumatoid arthritis have been reported to show higher Gal1 serum levels than the healthy population. Our study aimed to find genetic variants on the Gal1 gene (LGALS1) modulating its expression and/or clinical features in patients with early arthritis (EA). LGALS1 was sequenced in 53 EA patients to characterize all genetic variants. Then, we genotyped rs9622682, rs929039, and rs4820293, which covered the main genetic variation in LGALS1, in 532 EA patients. Gal1 and IL-6 serum levels were measured by ELISA and Gal1 also by western blot (WB) in lymphocytes from patients with specific genotypes. Once disease activity improved with treatment, patients with at least one copy of the minor allele in rs9622682 and rs929039 or those with GG genotype in rs4820293 showed significantly higher Gal1 serum levels (p < 0.05). These genotypic combinations were also associated with higher Gal1 expression in lymphocytes by WB and lower IL-6 serum levels in EA patients. In summary, our study suggests that genetic variants studied in LGALS1 can explain heterogeneity in Gal1 serum levels showing that patients with higher Gal1 levels have lower serum IL-6 levels.     

高陡边坡危岩体治理技术

杨房沟水电站自然边坡高陡,边坡岩石节理发育,浅部岩体卸荷作用明显,局部岩体松动,山脊突出或边坡陡峻的局部形成了危岩体、危石群等,严重威胁下部建筑物、人员及设备安全.笔者通过分析危岩体特点,确定了危岩体治理的总体目标、原则及设计方法,提出了治理方案、施工技术及监测、预警等综合措施,解决了边坡稳定及安全问题.     

弹道导弹防御预警系统弹道预测误差分析

基于所建立的辅助坐标系,通过一系列坐标变换对弹道导弹中段防御预警系统的弹道预测过程进行了合理分解,有效地简化了预测状态的表达形式和计算过程.在此基础上对跟踪雷达正弦坐标系下弹道预测的误差进行了分析,给出了误差的工程计算方法和蒙特卡洛仿真结果.分析过程和仿真结果表明,利用该方法得到的预测误差与实际预测误差水平相吻合,其计算过程较传统方法简单、高效.     

低压系统短路电流快速分断控制实现的研究

本文分析了实现低压系统短路故障早期检测的主要难题,设计了基于短路故障早期检测模型的短路电流快速分断控制系统并进行了有效验证,解决了短路故障早期检测理论的实际应用问题。同时,为开发智能型保护电器提供了一个有效的试验验证方法。     

基于步进凝视控制策略的告警系统

针对近程导弹告警设备目前采用的连续扫描型存在成像质量不高和固定凝视型成本过高的问题,对一种面阵红外CCD探测器的探测方法进行了研究.提出将先进的非等速步进准凝视图像采集控制方法用于一种复合式小型导弹告警系统中.对常规控制方法和步进准凝视控制方法进行了比较,重点解决了该控制技术在红外探测系统中的应用.此方案在某型在研告警设备中进行了实验,方位误差限定在0.4 mrad条件下其分辨力可迭0.01°.该控制方法可应用到其他告警探测设备的设计中.     

石钢集中道岔平交道口自动报警控制系统

利用无源磁感应技术建立的铁路道口自动报警控制系统,实现了道口的无人值守,解决了石钢站场道岔集中区段内平交道口的交通难题。     

大规模多点测温中缩短巡检周期的一种DS18B20访问协议

针对运用DS18B20进行大规模多点测温时巡检周期过长问题,从工作过程分析了原因所在,据此提出采用报警窗口机制的新协议进行数据采集.并通过分析和实践所得数据的对比,就协议缩短巡检周期的有效性作了验证.另外,对可能出现的测温点传感器发生故障须考虑的信息处理问题,提出一种安全的应对方案.该协议运用于粮食仓储的温度检测系统现场,取得预期效果.     

飞机防撞系统的建模与仿真

随着空中交通的日益繁忙,民用飞机的空中防撞显得尤为重要。根据飞机空中防撞的要求,首先详细研究了防撞的预警系统,得出了以飞机应答信号为主要信息的预警判断算法。在算法的基础上建立飞机防撞预警系统的仿真模型。采用仿真一体化软件(FLSIM),设计了飞机防撞仿真系统,给出了仿真的详细流程。通过以B747为例的仿真实验,验证了TCAS防撞预警算法的正确性,证明预警有效性,并为进一步开展飞机防撞系统的研究打下了基础。