Sequence‐Specific Molecular Lithography Towards DNA‐Templated Electronics

Recent advances in the realization of individual molecular‐scale devices [1,2] highlight the integration of individual devices into large‐scale functional circuits as the major challenge. DNA‐programmed assembly is a promising avenue in that direction due to the large amount of information that can be coded into the molecules and the ability to translate that information into physical constructs [3]. Large‐scale DNA‐templated electronics require, however, complex manipulation of double‐stranded DNA (dsDNA) molecules, as well as patterning of the electrical properties instilled to them by, e.g., metallization. To that end, sequence‐specific molecular lithography on single DNA molecules has been developed [4]. This was achieved by harnessing the exquisite homologous recombination process of the RecA protein. Sequence‐specific patterning of the metal coating of DNA molecules, localization of arbitrary labeled molecular objects at any desired dsDNA address without prior modifications, and generation of molecularly accurate stable dsDNA‐dsDNA junctions are demonstrated. The information encoded in the DNA molecules directs the lithographic process in analogy to the masks used in conventional microelectronics. The RecA protein provides the assembling capabilities, as well as the resist function.     

房地产项目开发建设中住宅整体质量问题探析

商品住宅的质量关系着国家的未来和房地产业的兴旺,本文试图对住宅产品的质量现状、问题及成因进行分析,在此基础上提出进一步提高品住宅整体质量的看法:     

无键联接传动的特点及锥面弹性衬套设计和应用

无键联接在特殊行业和工况中是键联接传递动力和运动的有力补充,介绍了工程上应用的圆弧三边形型面联接、弹性环无键联接、圆柱面过盈联接等几种无键联接传动方式及其工作原理,并分析了各自的性能特点和应用情况。针对无键联接传动的特点、特殊行业和工况的应用实际,设计了锥面弹性衬套无键联接传动结构并介绍了其在工程中的实际应用。     

公路工程变更与索赔

介绍了公路工程施工中引起的各种索赔问题，阐述了索赔的方法与技巧，指出只有掌握和运用索赔理由和方法，才能有效地控制成本，提高经济效益。     

工程量清单招投标的实践探索

根据《中华人民共和国招标投标法》实施中的有关问题，探讨了采用工程量清单方式招投标的合理性和可行性，并给出了采用工程量清单法进行招投标的过程与步骤。     

Faculty and Student Attitudes Toward Community Service: A Comparative Analysis

The Humanitarian Engineering initiative, sponsored by the William and Flora Hewlett Foundation, at the Colorado School of Mines, is creating a program that will support engineering students in understanding their responsibility for solving community development problems that exist throughout the world. As part of this effort, data has been collected on faculty and student attitudes using the ‘Community Service Attitudes Scale,” developed and validated by Shiarella, McCarthy, and Tucker. During the fall 2004, 78 students and 34 faculty members responded to this instrument. Statistically significant differences were found between the attitudes of students and faculty, males and females, and among different age groupings with respect to service activities. A general finding was that faculty displayed better attitudes toward community service than the students.     

浅谈工程项目的预算(成本)管理

从施工单位管理的角度出发,在工程投标、施工前、施工中及完工等不同阶段对项目的预算管理重要性进行描述,充分表明了只有加强各个阶段的预算(成本)管理,才能真正提高经济效益。     

构皮滩水电站烂泥沟砂石系统设计与实践

烂泥沟砂石系统是构皮滩水电站主体工程混凝土骨料加工的主要基地,共需加工混凝土骨料约600万m3,其料源为观景料场玉龙山灰岩,湿抗压强度大于50 MPa。该系统的骨料破碎筛分采用三段破碎四级筛分工艺,人工制砂采用棒磨机与立式冲击破联合制砂工艺。本文结合该系统工艺设计与施工,对系统设计中的关键技术进行了分析与总结。