抗真菌蛋白Rs-AFP2基因定点突变及其对大肠杆菌中表达的影响

为了研究遗传密码子对表达调控的影响,利用PCR重叠延伸法,对萝卜抗真菌蛋白Rs-AFP2基因编码序列区的部分核苷酸进行沉默突变,构建突变体Rs-AFPm.序列分析表明,PCR产物全长240bp,有一个阅读框,编码的蛋白由29个氨基酸的信号肽和51个氨基酸的抗真菌蛋白组成.突变体与突变前的Rs-AFP2基因相比,在编码区第3号氨基酸Lys相差一个碱基(TTG→TTA),第5号氨基酸Gln相差一个碱基(CAG→CAA),第6号稀有密码子Arg相差两个碱基(CAG→CGA).重新合成引物,将切除信号肽的Rs-AFP2基因和Rs-AFPm基因与原核表达载体pET-21b(+)分别重组到大肠杆菌BL21菌株.IPTG诱导后,二者均得到了表达.软件分析显示,突变前pETAFPo表达产物占全菌蛋白的3%,突变后pETAFPm的表达产物占全菌蛋白含量的8%;表达蛋白主要以包涵体的形式存在,包涵体经超声波破碎后,蛋白质复性,抑菌结果表明,pETAFPm表达产物的抑菌半径大于pETAFP2表达产物的抑菌半径.这些都说明改造后的Rs-AFPm基因与Rs-AFP2基因相比,已有效地提高表达量.     

PKI/CA技术综述

公开密钥基础设施(PKI)是解决网络安全的技术．典型PKI应用系统包括安全Web、注册机构(RA)、认证机构(CA)、目录(LDAP)和数据库等服务器．RA验证客户证书申请后提交CA．CA检查信息完整和数字签名正确后把证书存放到证书库和目录服务器，并将签发证书序列号通知客户和RA．客户即可使用该号通过目录服务器下载证书．1993年以来，美、加、欧洲、韩、日等国已相继展开PKI研究．我国2001年将PKI列入十五863计划，并着手解决PKI标准化、CA互联互通等关键技术问题。     

Ku波段波导型双工器的研制

采用高精度的模式匹配法设计了一种Ku波段波导型双工器。双工器采用H面T接头和E面横向膜片波导带通滤波器。采用该方法设计的双工器精度高，结构简单，便于加工。文中也将理论结果和实测数据进行了比较，两者吻合得很好，证明了模式匹配法的精确性和有效性。     

High resistivity measurement of SiC wafers using different techniques

To establish fast, nondestructive, and inexpensive methods for resistivity measurements of SiC wafers, different resistivity-measurement techniques were tested for characterization of semi-insulating SiC wafers, namely, the four-point probe method with removable graphite contacts, the van der Pauw method with annealed metal and diffused contacts, the current-voltage (I-V) technique, and the contactless resistivity-measurement method. Comparison of different techniques is presented. The resistivity values of the semi-insulating SiC wafer measured using different techniques agree fairly well. As a result, application of removable graphite contacts is proposed for fast and nondestructive resistivity measurement of SiC wafers using the four-point probe method. High-temperature van der Pauw and room-temperature Hall characterization for the tested semi-insulating SiC wafer was also obtained and reported in this work.     

基于扩展分形和CFAR特征融合的SAR图像目标识别

研究了多信息融合技术在SAR图像目标识别中的应用。将扩展分形特征(Extended Fractal)与双参数恒虚警特征(Double Parameter CFAR)形成的多信息进行融合处理。运用Dempster-Shafer证据理论，在决策层对SAR图像中的像素进行识别分类。实验结果表明通过融合对像素分类的准确性明显好于单特征的检测结果，减少了虚警概率，提高了系统的识别能力。     

整合式有线电视的视频点播配置方法

提出一种视频点播系统传送数据的结构,结合单播与群播两种数据传送的方法,通过动态改变群播与单播的频道分配比例,让大部分的使用者无需等待即可开始观看影片,进而减少整体使用者的等待时间.     

Reconfigurable hardware implementation of a phase-correlation stereoalgorithm

This paper describes the implementation of a stereo-vision system using Field Programmable Gate Arrays (FPGAs). Reconfigurable hardware, including FPGAs, is an attractive platform for implementing vision algorithms due to its ability to exploit parallelism often found in these algorithms, and due to the speed with which applications can be developed as compared to hardware. The system outputs 8-bit, subpixel disparity estimates for 256× 360 pixel images at 30,fps. A local-weighted phase correlation algorithm for stereo disparity [Fleet, D. J.: {Int. Conf. Syst. Man Cybernetics 1:48–54 (1994)] is implemented. Despite the complexity of performing correlations on multiscale, multiorientation phase data, the system runs as much as 300 times faster in hardware than its software implementation. This paper describes the hardware platform used, the algorithm, and the issues encountered during its hardware implementation. Of particular interest is the implementation of multiscale, steerable filters, which are widely used in computer vision algorithms. Several trade-offs (reducing the number of filter orientations from three to two, using fixed-point computation, changing the location of one localized low-pass filter, and using L1 instead of L2 norms) were required to both fit the design into the available hardware and to achieve video-rate processing. Finally, results from the system are given both for synthetic data sets as well as several standard stereo-pair test images.     

CMP系统技术与市场

概述了CMP系统技术的发展历史、发展趋势以及在IC生产中的重要性,介绍了国外CMP设备主要制造厂家的设备型号和性能及CMP设备市场分布和需求,阐述了CMP系统技术的基础研究、关键技术和国内研究概况。     

基于光子带隙(PBG)结构的小型K波段谐振式带通滤波器

提出了一种新的基于光子带隙结构的K波段谐振式带通滤波器，给出了近似设计公式，并在半导体衬底上，采用微电子的工艺实现了这种结构。这种滤波器设计方法简单，体积小，整个长度接近谐振频率上的导波波长，加工方便，便于与其他电路集成，带内损耗小，阻带范围宽，带外抑制高。另外，本文的理论分析和实验结果均基于半导体衬底，可以直接应用于微波集成电路中。理论模拟与实际测量值基本吻合。