物联网技术于人文艺术之应用

近年来,物联网(Internet of Things,IoT)产业受到许多的关注,世界各国均视其为潜在无限商机的高科技产业,并投入大量的资源从事研发与推广。由于物联网的应用非常广泛,且透过物联网技术人类得以提升生活质量,让生活更加便利,因此,本论文首先简介物联网的背景及应用,并介绍其基本概念与架构。接着,本论文以「无线感测真菌人文树道」为例,针对物联网技术应用于人文艺术领域进行说明,并详细叙述其所使用到之各项软件、韧体及硬件技术,透过真菌的感测、无线通信及异质网络连网功能的设计,使真菌网络成为物联网在人文艺术应用的一个重要典范。     

高职计算机文化分专业分层次教学的实践与思考

指出了高职计算机文化课程教学面临的诸多问题,这些问题的存在严重制约了课程教学效果的提高.为此,在深入企业调研和对教学对象调查的基础上,结合各专业类别特点及其对计算机基本操作技能的不同需求,选取不同专业的班级作为研究对象,采用“横向分专业,纵向分层次”的教学方法进行了实践教学.实践结果表明,分专业分层次教学方法能有效解决高职“计算机文化”课程教学中存在的主要问题.     

文化自信视域下岭南文化创新发展探究 ——以广州文化建设为例

广州既是改革开放的前沿阵地,也是岭南文化的传播和发展中心。分析广州近年来在文化建设方面的实践经验,特别是在推动岭南文化、广府文化创造性转化和创新性发展方面的经验,对于进一步贯彻落实习近平总书记关于文化自信建设的重要论述精神,扎实推进文化建设具有重要作用。     

Traditional organizations and crisis management in transition—Organizational perspectives on new dynamics of independent civil engagement

Digitalization and the trend towards more short‐term and project‐oriented social engagement have had a direct impact on traditional crisis management. This societal change has supported the evolution of new types of volunteers who use low‐threshold potentials to organize and maintain support independently. Traditional institutions like the German Red Cross are challenged to deal with these rapid changes regarding their own organizational culture in relation to the new phenomenon of so‐called unaffiliated volunteers. This paper investigates how unaffiliated volunteers influence and change the organizational culture of German disaster management by analysing and reflecting organizational adaptation processes in the aftermath of the German floods in 2013, the storm events in the west of Germany in 2014 and the refugee relief mission in 2015–2016. Therefore, semi‐structured interviews with experts of the German Red Cross were held to analyse their reactions towards unaffiliated Volunteers during the Floods in 2013 and the refugee relief missions, and further focus group interviews were conducted to validate the extracted results. Findings suggest that among professionals and leading entities in the German Red Cross, a process of rethinking has emerged, recognizing the importance and necessity to open up organizational structures for the collaboration and coordination of unaffiliated volunteers.     

西藏网络文化阵地建设研究

本文对西藏的网络文化建设现状和问题进行了分析,指出加强西藏网络文化阵地建设的重要性和必要性,针对多渠道打造良好网络文化阵地建设的宣传普及和教育平台提出了一些建设方案,形成各种推动和谐网络文化建设的合力,推动西藏网络文化良好发展。     

试论国企思想政治工作与企业文化建设的融合

思想政治工作的高效开展对坚定党对企业的领导地位起到关键作用,能够充分发挥基层党支部政治优势和领导作用,推动企业科学发展。企业文化是企业管理理念和发展思想的直观呈现,营造积极的企业发展环境。两者间的练习较为紧密,两者的科学融合对企业发展起到有效的推动作用。通过对两者的关系进行详细阐述,分析融合工作开展的重要作用,探讨科学的融合工作开展策略,建设完善的融合工作体系,推动融合工作效果的提升,为企业发展提供有效支持。     

企业文化需要追求个性创新发展

企业文化理论从20世纪80年代提出以来,经过20多年的实践,如今发展快、口号多、落地难,有的演变为企业文化的“作秀”。要提升企业核心竞争力,必须在塑造优秀企业文化中,追求企业个性,避免雷同,求得创新发展。     

变流场情景下基于鱼类游泳特性的上溯通道分析

水电开发对洄游性鱼类的生境造成了影响,预测目标鱼类洄游期的上溯通道可为修建过鱼设施提供依据,从而减缓其负面效应。本文选取虎渡溪航电工程的下游河段作为研究区域,将长薄鳅、白甲鱼、异鳔鳅鮀作为目标鱼类,针对几种目标鱼类的游泳能力对不同流速进行分区后,得出了适用于多种过鱼目标的流速适宜度曲线,然后对研究区域过鱼期的典型流量进行了数值模拟。根据流速适宜度曲线对研究区域的多种过鱼目标进行了流速适宜度评价,并由评价结果预测了满足多目标过鱼要求的上溯通道。结果表明：流速适宜度评价最优区域出现的位置随流量变化而有所不同,低流量运行条件下出现在主流区域,而高流量运行条件下出现在主流区域两侧;上溯的通道面积占总面积的比例也随流量变化而有所不同,低流量运行条件下的占比约为高流量运行条件下的3 ~ 4倍。根据预测通道特征,对鱼道的进口布置及电站运行调度提供了建议。该结果可为相关鱼类的保护工作提供依据。     

Structural Studies of the Lipopolysaccharide of Aeromonas veronii bv. sobria Strain K133 Which Represents New Provisional Serogroup PGO1 Prevailing among Mesophilic Aeromonads on Polish Fish Farms

In the present work, we performed immunochemical studies of LPS, especially the O-specific polysaccharide (O-PS) of Aeromonas veronii bv. sobria strain K133, which was isolated from the kidney of carp (Cyprinus carpio L.) during an outbreak of motile aeromonad infection/motile aeromonad septicemia (MAI/MAS) on a Polish fish farm. The structural characterization of the O-PS, which was obtained by mild acid degradation of the LPS, was performed with chemical methods, MALDI-TOF mass spectrometry, and ¹H and ¹³C NMR spectroscopy. It was revealed that the O-PS has a unique composition of a linear tetrasaccharide repeating unit and contains a rarely occurring sugar 2,4-diamino-2,4,6-trideoxy-D-glucose (bacillosamine), which may determine the specificity of the serogroup. Western blotting and ELISA confirmed that A. veronii bv. sobria strain K133 belongs to the new serogroup PGO1, which is one of the most commonly represented immunotypes among carp and trout isolates of Aeromonas sp. in Polish aquacultures. Considering the increase in the MAI/MAS incidences and their impact on freshwater species, also with economic importance, and in the absence of an effective immunoprophylaxis, studies of the Aeromonas O-antigens are relevant in the light of epidemiological data and monitoring emergent pathogens representing unknown antigenic variants and serotypes.     

Visualizing Extracellular Vesicles and Their Function in 3D Tumor Microenvironment Models

Extracellular vesicles (EVs) are cell-derived nanostructures that mediate intercellular communication by delivering complex signals in normal tissues and cancer. The cellular coordination required for tumor development and maintenance is mediated, in part, through EV transport of molecular cargo to resident and distant cells. Most studies on EV-mediated signaling have been performed in two-dimensional (2D) monolayer cell cultures, largely because of their simplicity and high-throughput screening capacity. Three-dimensional (3D) cell cultures can be used to study cell-to-cell and cell-to-matrix interactions, enabling the study of EV-mediated cellular communication. 3D cultures may best model the role of EVs in formation of the tumor microenvironment (TME) and cancer cell-stromal interactions that sustain tumor growth. In this review, we discuss EV biology in 3D culture correlates of the TME. This includes EV communication between cell types of the TME, differences in EV biogenesis and signaling associated with differing scaffold choices and in scaffold-free 3D cultures and cultivation of the premetastatic niche. An understanding of EV biogenesis and signaling within a 3D TME will improve culture correlates of oncogenesis, enable molecular control of the TME and aid development of drug delivery tools based on EV-mediated signaling.