Quantitating Fluorescence Intensity from Fluorophore: The Definition of MESF Assignment

The quantitation of fluorescence radiance may at first suggest the need to obtain the number of fluorophore that are responsible for the measured fluorescence radiance. This goal is beset by many difficulties since the fluorescence radiance depends on three parameters 1) the probability of absorbing a photon (molar extinction), 2) the number of fluorophores, and 3) the probability of radiative decay of the excited state (quantum yield). If we use the same fluorophore in the reference solution and the analyte then, to a good approximation, the molar extinction drops out from the comparison of fluorescence radiance and we are left with the comparison of fluorescence yield which is defined as the product of fluorophore concentration and the molecular quantum yield. The equality of fluorescence yields from two solutions leads to the notion of equivalent number of fluorophores in the two solutions that is the basis for assignment of MESF (Molecules of Equivalent Soluble Fluorophore) values. We discuss how MESF values are assigned to labeled microbeads and by extension to labeled antibodies, and how these assignments can lead to the estimate of the number of bound antibodies in flow cytometer measurements.     

热流量计的原理

根据工业及家庭供暖的要求，设计并研制成一种新型的热流量计，它采用方便而新颖的采集信息方式，并使用CPU精确实时的进行控制和运算，保证了热流量计的精确、实时和“分户计量”。     

浮基多刚体系统动力分析

引入浮体势流流固耦合理论和多刚体力学的凯恩方法对浮基多刚体系统进行动力分析。考虑入射规则波的影响和上部作业物体进行回转作业、升降作业的影响，建立了浮基多刚体系统的运动方程，讨论了浮基多刚体系统运动方程在三类情况下的求解途径。     

桥梁颤振气动导数识别的迭代法

本文提出一种利用自由振动响应通过年顿－拉夫逊迭代同时识别出桥梁全部8个颤振气动导的方法。此法具有对迭代初值要求不高，识别结果稳定的特点，并具有一定的抗噪声干扰能力。数字仿真与实物试验结果表明本文方法有效，可行。     

纤维复合材料的热膨胀系数

提出了一种利用压电光声技术测量材料热膨胀系数的实验方法,并测试了单向复合材料C/C、C/Al的横向、纵向的热膨胀系数。根据已有的理论计算方法与实验结果对该方法的测试结果进行验证,证明了该检测方法的可靠性,进而又测量了C/C、C/Al材料在任一方向上的热膨胀系数。这种方法克服了理论计算过程复杂以及常规手段无法测量任一方向上热膨胀系数的缺陷。     

X射线衍射线形Fourier变换

采用计算机辅助实验技术，研究X射线衍射线开在仪器宽化修正过程中Fourier系数变化的规律及其影响，结果表明，只要实测线形接近Gauss 分布，Fourier变换就会导致弯勾效应；Forier变换可引起真实线形的尾部波动及大于实际值的宽化，引入边界条件可予以消除，Kα双线形经过Fourier变换后，Fourier纱数与真实Fourier系数存在着一定的差异。通过真实线形的Fourier变换可以修正。     

西北江三角洲来水来沙的非线性分形特征

根据西、北江干流高要、石角及西北江三角洲网河区顶点马口、三水四个水文站的流量和含沙量资料 ,应用非线性分形原理的R S方法 ,计算西北江干流及西北江网河区顶点四个水文站的流量、含沙量的赫斯特数H和分形维数D ;探讨西、北江干流来水来沙的分形特征及西北江三角洲的分水分沙规律。结果表明 ,流量的分维数略大于含沙量的分维数 ,三水站流量及含沙量的分维数与其上游的石角站流量、含沙量的分维数相差较大 ,与思贤的分水分沙造成西北江的水沙交换有关     

A VERSATILE CORRELATION OF LIQUID HOLD-UP IN TWO-PHASE COUNTER-CURRENT TRICKLE BED COLUMN

A new definition of the flooding point in a two-phase counter-current trickle bed column has been proposed. Based on this point a versatile correlation of liquid hold-up has been formulated. The coefficients of this correlation are invariant to the change of physical properties of liquid, type and size of the packing forming the bed and gas velocity. This conclusion has been reached from the analysis of experiments with four different liquids irrigating the bed and three types of packing of various size. The versatile correlation can be used also re-currently to calculate flooding hold-up and flooding liquid rate from two known values of liquid hold-up for a given gas rate.     

Variations in Spectral Slope in Lake Taihu,a Large Subtropical Shallow Lake in China

Spectral slope (S), describing the exponential decrease of the absorption spectrum over a given wavelength range, is an important parameter in the study of of chromophoric dissolved organic matter (CDOM) dynamics, and also an essential input parameter in remote sensing models. Furthermore, S is often used as a proxy for CDOM composition, including the ratio of fulvic to humic acids and molecular weight. The relative broad range in S values reported in the literature can be explained by the different spectral ranges and fitting methods used. A single exponential model is used to fit the S values for 17 investigations involving 458 samples in Lake Taihu from January to October in 2004. The average S value was 15.18 ± 1.39 μm⁻¹ for the range of 280–500 nm, which fell within the range reported in the literature. The frequency distribution of S value basically obeyed a normal distribution. Significant differences in S values between summer and other seasons showed that phytoplankton degradation was one of the important sources of CDOM in summer, whereas CDOM mainly came from the river input in other seasons. Furthermore, the estimated S value decreased with increasing wavelength range used in regression. The maximum and minimum values derived from the regression were 17.89 ± 1.25 μm⁻¹ and 13.62 ± 2.11 μm⁻¹ for the wavelength ranges of 280–380 nm and 400–500 nm, respectively, a decrease of 23.9%. S values significantly decreased with the increase of CDOM absorption coefficients. CDOM absorption coefficients could be more appropriately estimated from exponential model introducing the variation of S with absorption coefficients, making them useful for a remote sensing bio-optical model of Lake Taihu. DOC-specific absorption coefficient a*(λ) and the parameter M describing molecular size of the humic molecules could also be used as a proxy for the sources and types of CDOM. A general relationship was found between S and a*(λ), and M values. S increased with the decrease of DOC-specific absorption coefficient and the increase of M corresponding to the decrease of molecular weight.     

高青樊107联合站污水处理技术应用研究

根据胜利油田樊107区块低渗透油藏注水开发的需要，开展了高青樊107联合站污水处理技术研究，与辽宁华孚环境工程‘有限公司联合研发了一套适合樊107区块注水水质指标的污水处理工艺设备。结合高青樊107区块的地质特点、注水水质指标要求，介绍污水处理室内试验研究、工艺流程、工艺设备选型、现场试验、存在问题和整改措施。