磁控溅射羟基磷灰石薄膜的研究现状与展望

羟基磷灰石是具有良好生物相容性的骨修复和替代材料.介绍了用磁控溅射法制备羟基磷灰石薄膜的研究现状,由实验机理和薄膜的性能表征分析了影响薄膜质量的因素,并讨论了制备过程中存的一些问题以及今后的发展方向.     

Preparation of Hydroxyapatite Fibers by the Homogeneous Precipitation Method

This paper discussed the preparing process of hydroxyapatite fibers which were widely used as reinforcement for biomedical materials by homogeneous precipitation method.The needle-like hydroxyapatite crystals were synthesized in an aqueous system.They were transferred from precursors-dicalcium phosphate anhydrate and octacalcium phosphate crystals.The reaction conditions were well controlled in order to obtain crystals in given morphology.The products were characterized by X-ray powder diffractometry(XRD),scanning electron microscopy (SEM) and infrared spectroscopy(IR),They were verified to be ydroxyapatite crystals with needle-like in shape.     

A novel method to prepare mineralized fibroin fiber

In this paper, a novel method was developed to prepare mineralized fibroin fiber. We used fibroin gel to control the biomineralization of calcium phosphate and obtained one kind of mineralized fibroin fiber with the length of 1–2 mm. It has the potential to be used to enhance the strength of tissue engineering scaffold. Scanning electron microscopy (SEM) results showed that hydroxyapatite (HA) was mainly deposited on the surface of mineralized fiber. Fourier transform infrared spectroscopy (FTIR) results displayed the red shifts of absorption bands of amide II and amide III (9 and 5 cm⁻¹, respectively), which were related to the strong chemical interaction between HA and fibroin. It was also found that HA was at low content (12.5%) and the ability of gelled fibroin to induce mineralization decreased greatly because of the formation of β-structures in gelled fibroin molecules, which showed the importance of molecular structure in the regulation of the biomineralization process.     

撞击流-微乳液法纳米羟基磷灰石的制备

在一定pH值条件下，采用水溶液（W）／环己烷（O）／OP-10（S）／正辛醇（A）四元反相微乳液体系并结合撞击流技术制备出粒径为20-50nm的针状纳米羟基磷灰石（HA）晶体。红外光谱（IR）、X射线衍射（XRD）和透射电镜（TEM）对羟基磷灰石颗粒进行表征和分析表明：颗粒分散均匀，成针状，沿C轴方向的微晶尺寸约30nm，沿a轴方向为20nm左右。并主要考察了表面活性剂、杂质、反应和静置时间等对HA的微观形貌的影响。     

水热法合成羟基磷灰石晶须的工艺研究

纳米羟基磷灰石（HAP）增强聚合物基复合材料是替换硬组织的最理想材料，是植入材料的研究重点之一。为了制备与天然骨结构非常相似的复合材料，必须首先制备出性能优良的的纳米HAP粉体。对水热法制备HAP纳米晶须的工艺进行了研究，研究了反应时间、温度、起始反应物的浓度、钙磷比以及反应体系的pH值对合成HAP纳米晶须形貌的影响。用X射线衍射（XRD）、扫描电镜（SEM）和红外光谱（FT-IR）法，对制备的羟基磷灰石进行了表征和分析。结果表明：生成的HAP晶须的长度及长径比随反应时间的延长而增大。温度〈150℃时，得到HAP晶须；温度〉180℃，则得到细小HAP晶体的聚集产物。当反应物的pH=9时，得到的是缺钙型的HAP；当反应物的pH〈4时，产物中出现新相磷酸氢钙；当pH=2时，得到纯的磷酸氢钙。     

微乳液组分对体系增溶水量的影响及纳米羟基磷灰石的制备

应用电导率法研究了TritonX-100/正戊醇／环己烷体系中各组分及条件对微乳液最大增溶水量的影响。结果表明：当表面活性剂TritonX-100用量为3ml，助表面活性剂选正戊醇用量为1ml，环己烷为油相，温度为48℃，Ca（NO3）2溶液与（NH4）2HPO4溶液分别为0．24和0．14mol／L时，体系增溶水量最大，可得到较高产率的纳米羟基磷灰石。并且用XRD和TEM手段对产物进行了表征。     

含La羟基磷灰石生物活性膜层的制备与性能研究

采用微弧氧化法在含有乙酸钙、β-甘油磷酸钙、硝酸镧的电解液中在纯钛金属表面原位生成了多孔含La羟基磷灰石的生物涂层.用XRD、SEM、EDS、细胞培养等方法对膜层的厚度、物相、成分组成及生物相容性进行了研究.结果表明,膜层粗糙多孔,厚度在15μm左右,与钛基体之间无明显界面;膜层表面主要物相为含La的羟基磷灰石.细胞培养结果表明,膜层有良好的生物相容性.     

纳米羟基磷灰石对肝癌细胞端粒酶基因表达的影响

研究羟基磷灰石（HAP）纳米粒子对Bel-7402人肝癌细胞端粒酶基因表达的影响。采用均相沉淀法制备出稳定单分散的HAP纳米粒子,应用透射电镜、电位粒度仪对其进行表征。HAP纳米粒子作用Bel-7402肝癌细胞4 h后,采用原位杂交技术检测Bel-7402肝癌细胞的端粒酶基因表达。结果表明HAP纳米粒子作用组的Bel-7402肝癌细胞的端粒酶阳性细胞比例为61.38%,而对照组的端粒酶阳性细胞比例为87.89%,2组有显著性差异（P〈0.01）。HAP纳米粒子可使Bel-7402肝癌细胞的端粒酶基因表达下调。     

In situ synthesis of hydroxyapatite-grafted titanium nanotube composite

ABSTRACT

The present study is an investigation to demonstrate the effectiveness of in situ approach in the synthesis of hydroxyapatite-grafted titanium nanotube composite (HA-TNT). This method involves combining the process of HA sol–gel and rapid breakdown anodisation of titanium in a novel solution consisting of NaCl and N₃PO₄. This new synthesis approach produced a uniform dispersion of Anatase and Rutile phases of TiO₂ nanotubes with minimal agglomeration in the matrix of crystalline HA. The characterisation of homogenised HA-TNT composite was investigated via field emission scanning electron microscopy (FESEM), energy dispersive spectroscopy (EDS), transmission electron microscope (TEM) and X-ray diffraction (XRD). FESEM and TEM images indicated the nanostructure of composite with TiO₂ nanotube diameter of approximately 10 nm. XRD and EDS analyses confirmed the formation of HA crystalline with the Ca/P ratio of 1.58 and formation of Anatase and Rutile phase of TiO₂ nanotubes.     

仿生涂层形貌对MgZnSrCa合金降解速率的影响

镁合金降解速率过快限制了其作为生物医用材料的应用,对镁合金降解速率的控制成为了研究的热点。采用仿生法在MgZnSrCa合金基体表面形成羟基磷灰石涂层。利用X射线衍射仪、扫描电子显微镜及能谱仪对涂层结构、形貌和成分进行分析和观察。通过失重法、析氢法、pH值测定等方法,研究不同涂层形貌的合金试样在人体模拟体液(SBF)中的降解速率。实验结果表明:羟基磷灰石(HA)涂层可以降低合金的降解速率,可以通过控制涂层形貌对合金的降解速率进行控制。