Phosphorylation Stoichiometries of Human Eukaryotic Initiation Factors

Eukaryotic translation initiation factors are the principal molecular effectors regulating the process converting nucleic acid to functional protein. Commonly referred to as eIFs (eukaryotic initiation factors), this suite of proteins is comprised of at least 25 individual subunits that function in a coordinated, regulated, manner during mRNA translation. Multiple facets of eIF regulation have yet to be elucidated; however, many of the necessary protein factors are phosphorylated. Herein, we have isolated, identified and quantified phosphosites from eIF2, eIF3, and eIF4G generated from log phase grown HeLa cell lysates. Our investigation is the first study to globally quantify eIF phosphosites and illustrates differences in abundance of phosphorylation between the residues of each factor. Thus, identification of those phosphosites that exhibit either high or low levels of phosphorylation under log phase growing conditions may aid researchers to concentrate their investigative efforts to specific phosphosites that potentially harbor important regulatory mechanisms germane to mRNA translation.     

ZZ9900/29.5/50型支架护帮装置限压阀板的改进

对ZZ9900/29.5/50型支撑掩护式大采高液压支架护帮装置限压控制系统限压阀板存在的缺陷进行了分析、改进.     

边坡主要因素对强度折减法计算精度的影响

采用强度折减法和极限平衡法对粘土边坡进行稳定性分析,并将计算所得的安全系数加以对比,进行误差分析,通过研究粘聚力、内摩擦角、坡高和坡角等指标对计算精度的影响,揭示影响计算精度的主要因素和次要因素,为以后的数值模拟研究提供有利的参考。     

地应力影响因素三维数值模拟分析

以某水电站为实例,结合数值模拟,研究了河谷下切过程中断层、错动带以及侧向剥蚀对地应力的影响情况分析,为坝址区地应力特征研究提供了科学依据。通过计算得出:在断层、断层与断层及断层与层间错动带交错部位岩体应力明显减小,而侧向剥蚀主要影响左岸应力分布,对右岸应力分布的影响不是很明显。     

利用鸡蛋壳制备过氧化钙的工艺研究

以鸡蛋壳为钙源，制备过氧化钙，并考察了反应时间，蛋壳灰分的用量以及稳定剂（双氧水）的用量等因素对产物产率的影响。由实验结果得出最佳条件为反应时间20mm，稳定剂用量为21mL，蛋壳灰份用量为2．0g，其产率最高。最高产率为78．1％。     

工作辊护板颈部开裂原因分析与解决方案

针对某热轧中厚板轧机上工作辊护板在工作过程中颈部出现较深裂纹原因进行分析,以静力学平衡理论为基础,借助有限元分析和计算,找出裂纹产生的真正原因；在给出合理解决方案的同时,依托计算机模拟适当调整零件几何尺寸以提高零件薄弱区域强度,提高零件安全系数.     

A study on sensitivity correction factor of gamma camera in whole body bone image

The aim of this study was to calculate a reasonable correction factor that could be corrected when the sensitivity of each gamma camera is measured and a whole body bone scan is performed as a follow-up examination. Seven pieces of equipment were used to analyse the sensitivity measurements of each gamma camera based on the source sensitivity measurement method recommended by the IAEA. These were BRIGHTVIEW, PRECEDENCE, ECAM, ECAM signature, ECAM Plus, SYMBIA T2 and INFINIA. The ^99mTc line source for the sensitivity measurement was generated in 4–7 kcps, which are the common values in a whole body bone scan. All the cameras were equipped with a low-energy and high-resolution parallel multi-hole collimator and set at a window width and photo peak of 15% and 140 keV, respectively. After placing the ^99mTc source as closely as possible to the collimator, the count was measured for 60 and 120 seconds to calculate correction factor. To determine if the correction factor calculated using the ^99mTc line source could be applied to a whole body bone scan of a real patient, a whole body bone scan was performed on 27 patients before applying the correction factor for comparative analysis. According to the experimental results using the ^99mTc line source, the gamma camera sensitivity was the highest for ECAM plus, followed in order by the gamma cameras, ECAM signature, SYMBIA T2, ECAM, BRIGHTVIEW, INFINIA and PRECEDENCE. When the results were used to calculate the correction factor based on the ECAM gamma camera, which had a medium-degree of sensitivity, the respective correction factors were 1·07, 1·05, 1·03, 1·00, 0·90, 0·83 and 0·72. The correction factors calculated based on the experiment using a ^99mTc line source were similar to those calculated based on the whole body bone scan. Clinical application of correction factor for measured sensitivity enables estimation of factor correction depending on difference in equipment when image is read, which improves accuracy and reliability of examination, and is expected to be used especially for continuous follow-up examination.     

岸边集装箱起重机节能发电系统的设计

针对港口岸边集装箱起重机设计了一种新型高效的节能发电方案,利用简单有效的机械传动方式,在原有控制系统的基础上,增加部分闭环控制系统,实现无级变矩,使发电量最大化。运用ADAMS软件对下降过程中可调变速器的状态进行运动学仿真,并建立节能系统发电方案,以及控制流程。     

褐煤半焦燃烧的研究现状与发展

围绕褐煤半焦的燃烧特性,对国内外研究现状进行了阐述,对影响褐煤半焦燃烧特性的因素进行了分析,对褐煤半焦的燃烧利用提供了一定的理论基础。