是荧光还是散射光?是生物化学方法还是形态学方法?——与赖省生等同志商榷

本文提出:所谓“荧光扫描法”测定杂交水稻子一代种子纯度的方法,不是测定的荧光,而是测定的散射光,由种子表面对光的散射来进行测定。因而实质上仍然是一种形态学的测定方法。     

Prediction of venturi scrubber efficiency used in an updraft gasifier cleaning train

The performance of a venturi scrubber in the removal of tar from gas in updraft gasification has been studied. The gasifier has been operated with a husk feed rate of 1.6 × 10^?4 kg/s. The venturi scrubber has been operated at a superficial gas velocity of 56.4 m/s at the throat. A wide variety of scrubbing liquids having surface tensions ranging from 0.026 to 0.072 N/m have been used. The Q_g/Q_l, has been varied in the range of 1000–8000. The tar separation efficiency η has been found to vary from 51 to 98.5%. A mathematical model, assuming steady-state operation, has been developed considering very high pseudosolubility of tar in the scrubbing liquids. The predicted values of η have been compared with experimental results. The model satisfactorily explains the tar removal efficiency of the venturi for Q_g/Q_l values ranging from 4000 to 8000 for all scrubbing liquids. The following correlation has been developed for predicting venturi scrubber efficiency: .     

Cytological Observations and Bulked-Segregant Analysis Coupled Global Genome Sequencing Reveal Two Genes Associated with Pollen Fertility in Tetraploid Rice

Neo-tetraploid rice with high fertility is a useful germplasm for polyploid rice breeding, which was developed from the crossing of different autotetraploid rice lines. However, little information is available on the molecular mechanism underlying the fertility of neo-tetraploid rice. Here, two contrasting populations of tetraploid rice, including one with high fertility (hereafter referred to as JG) and another with low fertility (hereafter referred to as JD), were generated by crossing Huaduo 3 (H3), a high fertility neo-tetraploid rice that was developed by crossing Jackson-4x with 96025-4x, and Huajingxian74-4x (T452), a low fertility autotetraploid rice parent. Cytological, global genome sequencing-based bulked-segregant (BSA-seq) and CRISPR/Cas9 technology were employed to study the genes associated with pollen fertility in neo-tetraploid rice. The embryo sacs of JG and JD lines were normal; however, pollen fertility was low in JD, which led to scarce fertilization and low seed setting. Cytological observations displayed low pollen fertility (25.1%) and approximately 31.3 and 27.2% chromosome lagging at metaphase I and II, and 28.8 and 24.8% chromosome straggling at anaphase I and II in JD, respectively. BSA-seq of F_2–3 generations and RNA-seq of F₄ generation detected a common fragment, i.e., 18,915,234–19,500,000, at chromosome 7, which was comprised of 78 genes associated with fertility. Among 78 genes, 9 genes had been known to be involved in meiosis and pollen development. Two mutants ny1 (LOC_Os07g32406) and ny2 (LOC_Os07g32040) were generated by CRISPR/Cas9 knockout in neo-tetraploid rice, and which exhibited low pollen fertility and abnormal chromosome behavior. Our study revealed that two unknown genes, LOC_Os07g32406 (NY1) and LOC_Os07g32040 (NY2) play an important role in pollen development of neo-tetraploid rice and provides a new perspective about the genetic mechanisms of fertility in polyploid rice.     

静脉产业园餐厨垃圾处理厂与焚烧厂协同处理运行实例和经济性分析

以广西某静脉产业园内餐厨垃圾处理厂为例,介绍了以"预处理+油脂提取+厌氧发酵+沼气净化利用"为主体的餐厨垃圾处理工艺,并对该厂与生活垃圾焚烧厂协同处理进行了经济性分析。结果表明,与餐厨厂独立运营相比,与焚烧厂协同处理年节省成本达94.83万元。     

不同基质配比对银毛野牡丹扦插的影响

银毛野牡丹是优良的园林观赏性植物,但是繁殖有性结实率低。通过为期30天的扦插试验,对比不同基质（原土、草炭土、蛭石、珍珠岩）配比对银毛野牡丹插穗生长的影响,分析插穗生根时间、生根数目、最长根长、生根率、生长状态以及基质变化,找出适合其生长发育的优良基质,为银毛野牡丹的繁殖提供技术支撑。研究表明：试验组T8（原土：细沙：草炭土：蛭石：珍珠岩=1：1：2：1：1）的插穗生根率、最长根长、插穗长势为最佳,试验组T2（草炭土）效果次之,在条件有限的情况下可以作为备用方案以供生产所需。     

腐解稻草DOM提取液洗脱六价铬污染土壤试验研究

工矿企业产生的铬渣、含铬废水由于管理不善导致土壤铬污染严重,迫切需要寻求低成本铬污染土壤修复技术。提出腐解稻草中溶解性有机质（Dissolved Organic Matter, DOM）提取液洗脱铬污染土壤的技术,通过实验室模拟试验,以2种铬污染土壤（总铬含量为分别为121.54、941.90 mg/kg,六价铬含量分别为119.90、856.90 mg/kg）为研究目标,采用振荡淋洗法考察了固液比、淋洗时间、淋洗次数等因素对土壤中总铬、六价铬洗脱效果的影响,并探讨了淋洗前后铬形态变化及淋洗后土壤残留六价铬的持续钝化能力。研究结果表明：稻草经35 d腐解后的DOM提取液洗脱土壤中铬效果最佳,确定为最佳淋洗剂;固液比1：15、淋洗4h/次、淋洗2次为最佳淋洗工艺条件,该条件下,2种铬污染土壤总铬分别洗脱了47.79%、85.92%,六价铬含量分别削减了51.76%、95.09%;DOM提取液呈弱碱性,包含大量羧基、羟基及酚类物质,对水溶态、弱酸提取态和残渣态铬有较好的洗脱效果,有效降低了土壤环境风险,且能持续钝化淋洗后土壤中残余的六价铬。     

多回路冷凝法技术在聚丙烯装置尾气回收中的应用

陕西延长石油集团炼化公司延安石油化工厂,结合其20万t/a聚丙烯装置实际,自主开发出了一种多回路冷凝装置尾气的回收工艺技术,并成功工业化应用于该装置的3路丙烯尾气的冷凝回收系统。近5年的运行结果表明:经多种措施及操作优化后,该3路冷凝丙烯尾气回收系统,不仅实现了长周期连续平稳、安全运行,还可有效回收丙烯、丙烷分别约为2660,140 t/a。     

基于配额制的自备电厂参与新能源消纳日前交易模式

国内风电和光伏等新能源发电迅速发展,而部分地区本地消纳和电网外送能力均有限,当地自备电厂未充分发挥调峰作用,出现日趋严重的弃风弃光现象,因此难以完成配额制目标。为利用市场手段充分调动当地自备电厂参与新能源消纳,促进新能源配额制目标的完成,同时避免因强行完成指标导致的社会整体消纳成本升高的问题,提出了一种基于竞价摘牌的新能源日前交易模式。通过具有引导效应的市场化手段,调动具备自备电厂用户的主动消纳意愿,进而提升配额制目标完成度,并降低整体消纳成本。最后,以新疆地区算例为例,分析验证了所提交易模式在提升配额制目标完成度及在尽量降低社会整体消纳成本方面的价值,为新疆等类似地区电力市场建设提供了一种新的思路。     

Structural Investigation and Molecular Modeling Studies of Strobilurin-Based Fungicides Active against the Rice Blast Pathogen Pyricularia oryzae

The increasing emergence of fungicide-resistant pathogens requires urgent solutions for crop disease management. Here, we describe a structural investigation of new fungicides obtained by combining strobilurin and succinate dehydrogenase inhibitor pharmacophores. We identified compounds endowed with very good activity against wild-type Pyricularia oryzae, combined in some cases with promising activity against strobilurin-resistant strains. The first three-dimensional model of P. oryzae cytochrome bc1 complex containing azoxystrobin as a ligand was developed. The model was validated with a set of commercially available strobilurins, and it well explains both the resistance mechanism to strobilurins mediated by the mutation G143A and the activity of metyltetraprole against strobilurin-resistant strains. The obtained results shed light on the key recognition determinants of strobilurin-like derivatives in the cytochrome bc1 active site and will guide the further rational design of new fungicides able to overcome resistance caused by G143A mutation in the rice blast pathogen.     

Catalase (CAT) Gene Family in Rapeseed (Brassica napus L.): Genome-Wide Analysis,Identification, and Expression Pattern in Response to Multiple Hormones and Abiotic Stress Conditions

Catalase (CAT) is an antioxidant enzyme expressed by the CAT gene family and exists in almost all aerobic organisms. Environmental stresses induce the generation of reactive oxygen species (ROS) that eventually hinder plant growth and development. The CAT enzyme translates the hydrogen peroxide (H₂O₂) to water (H₂O) and reduce the ROS levels to shelter the cells’ death. So far, the CAT gene family has not been reported in rapeseed (Brassica napus L.). Therefore, a genome-wide comprehensive analysis was conducted to classify the CAT genes in the rapeseed genome. The current study identified 14 BnCAT genes in the rapeseed genome. Based on phylogenetic and synteny analysis, the BnCATs belong to four groups (Groups I–IV). A gene structure and conserved motif analysis showed that Group I, Group II, and Group IV possess almost the same intron/exon pattern, and an equal number of motifs, while Group III contains diverse structures and contain 15 motifs. By analyzing the cis-elements in the promoters, we identified five hormone-correlated responsive elements and four stress-related responsive elements. Further, six putative bna-miRNAs were also identified, targeting three genes (BnCAT4, BnCAT6, and BnCAT8). Gene ontology (GO) enrichment analysis showed that the BnCAT genes were largely related to cellular organelles, ROS response, stimulus response, stress response, and antioxidant enzymes. Almost 10 BnCAT genes showed higher expression levels in different tissues, i.e., root, leaf, stem, and silique. The expression analysis showed that BnCAT1–BnCAT3 and BnCAT11–BnCAT13 were significantly upregulated by cold, salinity, abscisic acid (ABA), and gibberellic acid (GA) treatment, but not by drought and methyl jasmonate (MeJA). Notably, most of the genes were upregulated by waterlogging stress, except BnCAT6, BnCAT9, and BnCAT10. Our results opened new windows for future investigations and provided insights into the CAT family genes in rapeseed.