Use of exogenous fibrolytic enzymes to enhance in vitro fermentation of alfalfa hay and corn silage

Two in vitro experiments were performed to identify promising exogenous fibrolytic enzyme products (EFE) and optimum dose rates (DR) for improving the degradation of alfalfa hay and corn silage. The relationship between enzymatic activity and fermentation responses was examined to identify optimum formulations. In experiment 1, 5 EFE containing mainly endoglucanase and xylanase activities, with different ratios between the 2 activities, were assessed at a DR of 0.7, 1.4, and 2.1 mg/g of DM forage. Milled alfalfa hay or corn silage was incubated in an in vitro batch culture with buffer, ruminal fluid, and EFE. Gas production (GP) was measured during 24 h of incubation, and degradabilities of DM and fiber were measured after terminating the incubation at 24 h. Two (E1 and E3) EFE substantially improved GP and degradation of alfalfa hay and corn silage fiber. The optimum DR of these EFE was 1.4 mg/g of DM for both forages with improvements in NDF degradability up to 20.6% for alfalfa hay and up to 60.3% for corn silage. Whereas added activities of endoglucanase and exoglucanase were positively correlated with improvement in NDF degradability for alfalfa hay and corn silage, there was no relationship between added xylanase activity and NDF degradability. The 2 most promising EFE from experiment 1 were reevaluated in experiment 2, alone and in combination with a high xylanase EFE, to determine whether their effectiveness could be enhanced by decreasing the endoglucanase to xylanase ratio. The 2 EFE improved GP and fiber degradation in a manner similar to that observed in experiment 1, but the combination treatments resulted in no further beneficial effects. Exogenous fibrolytic enzyme products can greatly improve forage utilization, but DR and the activities supplied are critical for achieving this response. Products used with alfalfa hay and corn silage should contain high endoglucanase activity, with an ideal ratio of endoglucanse to xylanase.     

The effects of Lactobacillus buchneri 40788 and Pediococcus pentosaceus R1094 on the fermentation of corn silage

The effect of inoculating whole-plant corn at the time of harvest with Lactobacillus buchneri 40788 (4 × 10⁵ cfu/g of fresh forage) combined with Pediococcus pentosaceus R1094 (1 × 10⁵ cfu/g) on the fermentation and aerobic stability of corn silage (37% dry matter) through 361 d of ensiling was investigated. Dry matter recovery was similar between treatments throughout the study except at one early time point (14 d), when treated silage had a lower recovery than untreated silage. The concentration of lactic acid was unaffected by inoculation but inoculated silages had greater concentrations of 1,2-propanediol and acetic acid from 56 to 361 d of storage. In general, inoculation decreased the concentration of water-soluble carbohydrates but increased the concentration of ethanol. The numbers of yeasts was lower in inoculated silage at 42, 56, 70, and 282 d of ensiling. However, inoculation did not consistently improve the aerobic stability of silage, suggesting that microbes other than yeasts may have been responsible for aerobic instability in this study. Even after prolonged storage (361 d), silage treated with L. buchneri 40788 and P. pentosaceus R1094 had normal silage fermentation characteristics.     

玉米胚芽蛋白及其在食品工业中的应用

概述玉米胚芽蛋白的营养价值和功能性质,并介绍玉米胚芽蛋白在焙烤食品、肉制品、饮料等方面的应用。     

用HPLC测定酶解玉米蛋白氨基酸的组成

利用高效液相色谱对玉米黄粉蛋白的酶解产物进行氨基酸组成测定，发现酶解产物中含有大量的Pro和Phe、Leu等必需氨基酸，因此可以用作保健食品来补充必须氨基酸和降低高血压患者的血压，具有广阔的应用前景。     

玉米油精炼过程氯离子含量变化及其对3-氯丙醇酯和缩水甘油酯含量的影响

对取自安徽、河北、山东等地玉米油加工厂的玉米油毛油、待脱臭油和成品油中的氯离子、3-氯丙醇（3-monochloro-1,2-propanediol,3-MCPD）酯和缩水甘油酯（glycidyl esters,GEs）含量进行检测,并在实验室明确可控的条件下对玉米毛油进行精炼,检测精炼过程氯离子、3-MCPD酯和GEs含量,与玉米油加工厂实际精炼过程的含量变化情况进行对比分析,研究玉米油精炼过程中氯离子含量变化及其对3-MCPD酯和GEs含量变化的影响。结果表明：所检测的所有玉米油样品中均含有氯离子、3-MCPD酯和GEs,其中毛油中氯离子含量最高,为0.628～2.029 mg/kg,毛油中3-MCPD酯和GEs含量分别为0.547～1.083 mg/kg和0.246～0.721 mg/kg;待脱臭玉米油中氯离子含量为0.110～0.374 mg/kg,较毛油中含量明显降低,3-MCPD酯和GEs含量分别为0.933～1.422 mg/kg和0.246～0.432 mg/kg,3-MCPD酯较毛油中含量有所升高,GEs含量变化不明显。脱臭油中3-MCPD酯和GEs含量分别为3.523～4.541 mg/kg和1.501～13.584 mg/kg,较待脱臭油中含量大幅升高,其中一个油样中含量分别增加3.5 倍和32.1 倍,同时该油样中氯离子含量的降幅也最大（0.287 mg/kg）。对实验室精炼过程及工厂精炼过程的玉米油样品检测分析显示,无论是实验室精炼还是工厂实际生产,氯离子含量均随精炼过程逐渐减少,其中降幅最大的是水化脱胶和碱炼脱酸工序（降幅为76.1%～81.3%）;3-MCPD酯和GEs在水化脱胶、碱炼脱酸及吸附脱色过程的含量变化不大,经脱臭后含量大幅升高,同时伴随脱臭过程氯离子含量的降低,并且氯离子含量降幅越大3-MCPD酯的升幅越高。研究结果明确了玉米油精炼过程氯离子和3-MCPD酯及GEs含量的变化规律,以及待脱臭玉米油中氯离子含量对脱臭油脂中3-MCPD酯和GEs含量的影响,对玉米油精炼乃至其他植物油生产中3-MCPD酯和GEs的风险防范和控制都具有指导意义。     

利用一株凝结芽孢杆菌发酵酸解玉米秸秆生产乳酸

该研究拟考察凝结芽孢杆菌（Bacillus coagulans）利用玉米秸秆生物炼制乳酸的效果。以凝结芽孢杆菌CGMCC No.7635为菌种,利用2%硫酸预处理后的玉米秸秆为碳源、20 g/L酵母粉为氮源,添加20 FPU/g纤维素酶后开展糖化发酵生产乳酸实验。结果表明,发酵65 h后可获得乳酸含量为（38.38±1.03） g/L,其中L-乳酸光学纯度为（99.23±0.22）%。进一步使用补料发酵工艺,添加经预处理的玉米秸秆,可最终获得乳酸含量为（82.56±1.28） g/L。建立的玉米秸秆生物炼制乳酸工艺操作简单、产物浓度高,具有工业应用潜力。     

稻田渍水及土壤水分平衡对春玉米生长影响的模拟分析

借助Penning de Vries的MACROS模型和1995～1997年田间及水槽实验结果，建立了玉米生长发育及水分动态耦合的模拟模型，模型模拟的玉米地上部干物质积累、土壤水分变化状况同实际较为符合。验证后的模型用于模拟不同渍水时期及持续时间对春玉米生长及产量影响的动态。模拟结果表明在田间全程控制水分为田间持水量90%情况下，春玉米孕穗期为渍水危害的敏感期，其次为4～6叶期。在自然降水及土壤渍水状况影响下，春玉米幼苗期4～6叶时为渍水的敏感期，8叶及孕穗期相对较耐渍。在玉米4、6叶期时，渍害造成产量下降的临界期为5d；而在8叶、孕穗期为10～15d.但持续20d的渍水对任何时期的春玉米生长都造成严重的影响。因此在生产中应注意在苗期及孕穗期及时排除田间多余的水分及降低地下水位。     

玉米芯作辅料酿造酱油的研究

为降低酱油生产成本,介绍了用玉米芯作辅料生产酱油的工艺。着重介绍了原料处理、盘制曲和厚层通风制曲、低盐固态发酵法和高盐稀醪发酵法等工艺的操作要点。利用上述方法生产出的酱油都达到了标准的要求。     

空气源热泵热水系统在冬冷夏热地区的应用探讨

空气源热泵热水系统是一种高效的建筑节能方式,但其工作过程复杂,投资昂贵.探讨了如何在冬冷夏热的长江中下游地区合理选配热泵热水系统,达到最佳技术经济效益.认为在该地区,热泵热水机组的配用功率应以春秋季气温平均值配置更合理     

基于全极化雷达数据反演夏玉米覆盖农田表层土壤含水量

基于RADARSAT-2全极化C波段雷达数据和野外实测数据,建立了不同极化方式的后向散射系数与夏玉米植被含水量的响应关系,获得了山东禹城夏玉米覆盖区域农作物含水量。在此基础上,对＂水—云模型＂的参数进行了修改,从总的极化雷达后向散射中去除了植被影响,获取了土壤组分的后向散射系数,从而能够通过雷达多极化数据得到表层土壤含水量。结果表明：夏玉米植被含水量观测值与VH、VV后向散射系数的相关性均达到显著水平,相关系数分别为0.86和0.79,VH极化优于VV极化;在RADARSAT-2四种极化方式下,VV极化下的＂水—云模型＂理论计算的误差平方和最小,是研究区表层土壤含水量反演的最优极化方式。