Targets and methods for the detection of processed animal proteins in animal feedstuffs

Bovine spongiform encephalopathy (BSE), or ‘mad cow disease’, is one of several transmissable spongiform encephalopathies (TSEs) known to affect certain mammals and is spread through the ingestion of infected animal tissue. It is believed that the inadvertent contamination of meat and bone meal (MBM) with infected animal tissue and the subsequent use of this material as a feed supplement contributed to the spread of the disease in cattle. As a result, the use of processed animal proteins (PAPs) in animal feeds is regulated in many parts of the world. Although feed testing is the only definitive means to certify compliance, regulatory compliance often relies solely on paper certification. Recently, rapid methods have become available that can be used by regulators to determine compliance during routine inspections. We describe a rapid, immunochromatographic strip test that can detect 0.1% MBM in animal feed. The test takes 15 min to perform and large numbers of samples can be screened for PAPs simultaneously.     

热焊弯管用钢板工艺探讨

在统计分析、正交多项式回归和模拟试验的基础上，建立了高精度的屈强比预报模型，并运用多元函数极值原理，求导极值点及相应的工艺参数，为优化工艺制度，提高热焊弯管用钢(L360-WG系列)性能合格率提供了定量依据。     

Effects of different cooking methods on the formation of food mutagens in meat

Hamburgers and chicken fillets were cooked in convection ovens, deep‐fried or contact fried and analysed for mutagenic activity using the Ames test. For the three different convection ovens, the cooking parameters studied included the presence of steam, air velocity, air temperature and holding time. For deep‐frying and contact frying, the cooking parameters included cooking temperature and cooking time. In cooked hamburgers, mutagenic activity was only detected in those that had been deep‐fried. In chicken fillets, mutagenic activity was detected in samples prepared with all cooking methods, being highest in the deep‐fried samples. Factorial analysis indicated that heat transfer was the most important factor affecting mutagenic activity. High temperature and high air velocity in the convection ovens enhanced mutagenic activity. The presence of steam reduced the mutagenic activity, except when high temperature was used in combination with high air velocity. In chicken fillets, high mutagenic activity correlated to high weight loss during cooking. Pan‐fried chicken fillets were analysed using high performance liquid chromatography (HPLC) and the mutagenic/carcinogenic heterocyclic amines 2‐amino‐3,8‐dimethylimidazo[4,5‐f ]‐quinoxaline (MeIQx), 2‐amino‐1‐methyl‐6‐phenylimidazo‐[4,5‐b]‐pyridine (PhIP) and the co‐mutagenic heterocyclic amine Norharman (9H‐pyrido[3,4‐b]‐indole) were identified. The HPLC fractions were tested for mutagenic activity and, apart from the mutagenic fractions corresponding to MeIQx and PhIP, several mutagenic fractions were detected that did not correspond to known heterocyclic amines.     

维氏硬度检测中不确定度初探

通过分析维氏硬度检测中的不确定度，计算了各不确定度分量所占的权重，得到HVlO(440--738)范围内的相对不确定度为1．3％--1．7％。     

Self-controlled case series analyses: Small-sample performance

Second-order expressions for the asymptotic bias and variance of the log relative incidence estimator are derived for the self-controlled case series model in a simplified scenario. The dependence of the bias and variance on factors such as the relative incidence and ratio of risk to observation period are studied. Small-sample performance of the estimator in realistic scenarios is investigated using simulations. It is found that, in scenarios likely to arise in practice, asymptotic methods are valid for numbers of cases in excess of 20-50 depending on the ratio of the risk period to the observation period and on the relative incidence. The application of Monte Carlo methods to self-controlled case series analyses is also discussed.     

A disposable single-use electrochemical sensor for the detection of uric acid in human whole blood

We report here a non-enzymatic detecting electrode strip for fast monitoring of uric acid in human whole blood. A single-use amperometric uric acid sensor strip, incorporating a three-electrode configuration, has been fabricated on a polypropylene substrate using low cost screen-printing (thick-film) technology. Both the working and counter electrodes were prepared by screen-printing commercial carbon ink. The integration on the same support of pseudo-reference electrode was obtained by screen-printing a commercial silver ink and subsequent electrochemical pretreatment. Simply by placing a 20 μl human whole blood drop on the strip is enough for uric acid analysis by square-wave voltammetry. Real human whole blood samples were analyzed by this method and compared to the phosphotungstic acid clinical test procedure with satisfactory results.     

光电对抗装备发展趋势

通过未来战场对光电对抗装备的作战要求和装备现状分析，提出光电对抗装备发展趋势。     

广播电视发射台技术指标集中自动测试系统

本文介绍了利用计算机技术、网络技术、三遥技术对广播电视发射技术指标进行实时自动测试的系统，以便进一步为安全优质播出提供技术保证。     

50kg／m钢轨轨底缺陷的检验和分析

钢轨轨底缺陷有三种：底伤、结疤和底裂缺陷。取２０根有底缺陷５０ｋｇ／ｍＵ７１Ｍｎ钢轨做试样，通过低倍、断口、金相检验和扫描电能谱分析，详细描述了轨底缺陷的宏观和微观特征，研究出钢轨轨底缺陷出现的规律臁其产生的原因。通过对比试验，发现淄博１号保护渣有利于改善钢锭表面质量，提高钢轨一级品率。     

SW233 PIN驱动器自动测试系统的研制

介绍了一种集成电路自动测试系统，该系统采用计算机并口作通信接口，用VB6编程，实现了对外围测试电路的控制，用IEEE-488接口卡控制测试仪器，可对SW233电路的36个参数进行自动测试，并将测试结果自动保存在数据库中。该测试系统具有自动化程度高、操作方便、测试结果精确等特点。