The microscopy cell (MicCell), a versatile modular flowthrough system for cell biology, biomaterial research, and nanotechnology

We describe a novel microfluidic perfusion system for high-resolution microscopes. Its modular design allows pre-coating of the coverslip surface with reagents, biomolecules, or cells. A poly(dimethylsiloxane) (PDMS) layer is cast in a special molding station, using masters made by photolithography and dry etching of silicon or by photoresist patterning on glass or silicon. This channel system can be reused while the coverslip is exchanged between experiments. As normal fluidic connectors are used, the link to external, computer-programmable syringe pumps is standardized and various fluidic channel networks can be used in the same setup. The system can house hydrogel microvalves and microelectrodes close to the imaging area to control the influx of reaction partners. We present a range of applications, including single-molecule analysis by fluorescence correlation spectroscopy (FCS), manipulation of single molecules for nanostructuring by hydrodynamic flow fields or the action of motor proteins, generation of concentration gradients, trapping and stretching of live cells using optical fibers precisely mounted in the PDMS layer, and the integration of microelectrodes for actuation and sensing.     

Theoretical Investigation of Time Suboptimal Control of Industrial Manipulators Along Specified Paths

A method for the time suboptimal control of an industrial manipulator from an initial position and orientation to a final position and orientation as it moves along a specified path is proposed. Nonlinear system equations that describe the manipulator motion are linearized at each time step along the path. A method which gives the control inputs (joint angular velocities) for time suboptimal control of the manipulator is developed. In the formulation, joint angular velocity and acceleration limitations are also taken into consideration. A six degree of freedom elbow type manipulator is used in numerical examples to verify the method developed.     

高压低温先导式安全阀的设计

介绍了一种某试验系统用高压低温先导式安全阀,主要从工作原理、结构特点、材料的选用、结构特点、试验等方面进行阐述。     

位置控制器与通用变频器APC系统

在每一周期的开始时的速度值V1预设定到传动装置上进行速度预控制，同时，反馈回来的实际值Sj与S1进行比较计算，得出当时偏差值△S，此偏差值△S乘以比例系数Ksp，得到Vk叠加在Vt上，得出传动装置应具有的速度控制信号V，然后V经速度／电压变换和D／A转换向传动系统的速度控制发出控制信号Uα控制执行机构的定位。调节Ksp的值使系统无超调无振荡。     

炼钢中间包塞棒液压伺服比例控制系统的应用

济钢第三炼钢厂中间包塞棒液压伺服比例控制系统采用伺服比例阀和伺服缸等,系统设计完善,功能齐全,可实现塞棒位置的闭环控制,保证了结晶器中钢水液面的稳定。     

达县广电城域网运行状况及故障分析

对达县广电城域网运行状况进行了检测，认为不能满足用户上网需要的原因除了网内病毒感染之外，设备与设备之间链路层协商及连接线出现故障。改进措施主要有两点：解决NE08E与S8505的连接问题，解决S8505上的DHCP服务器不能分配IP地址问题。     

蜂窝移动通信系统核心网络的演进与全IP核心网络

分析了现有移动通信网络存在的问题，阐述了第2代移动通信核心网向第3代移动通信核心网演进的必然性：讨论了第2代移动通信核心网向第3代移动通信核心网演进所采用的演进策略，介绍了两种可供选择的全IP核心网络的参考结构。     

汽车发动机电机铁芯用钢(ADW2)的研制开发

鞍钢研制开发的汽车发动机启动电机用钢是针对汽车电机铁芯用钢（定、转子）而开发的电机钢类产品。介绍了该产品的研制过程及规格、特点。通过批量试制及装机试验，产品巳满足用户的使用要求。     

位置灵敏探测器数据采集的软件设计

介绍了一个基于Windows操作系统的数据采集软件，该软件用于使用重心法确定的位置信息的位置灵敏探测器，具有方便的人机交互界面，使用专用驱动程序采集数据。     

柴油机针阀体的乙炔低压渗碳

乙炔低压渗碳可以有效地避免在真空渗碳过程中产生炭黑和焦油，同时由于乙炔具有较高的供碳能力，使这一技术尤其适用于针阀体这样的小直径深盲孔零件。