Liquid–liquid extraction of a recombinant protein with reverse micelles

Recombinant cytochrome b₅ was extracted into the reversed micelle phase of an anionic surfactant (AOT) in octane and back-extracted to a final aqueous phase. The extraction of the protein was controlled by an electrostatic mechanism, since it was dependent on the global charge of the protein. This was directly demonstrated by experiments with native and mutant cytochromes obtained by site directed mutagenesis. The back-extraction of cytochrome b₅ to a fresh aqueous phase was decreased by factors that reduced the size of the water pool of the organic phase, such as high salt concentrations (1–2 mol dm^?3 NaCl) and low temperatures (4°C), probably because of an increase in a favourable interaction of this protein with the surfactant at closer distances.     

Enantioselective extraction of mandelic acid enantiomers based on chiral ligand exchange

Based on the chiral ligand exchange, the distribution behavior of mandelic acid enantiomers, and the partition of Cu^2 at different pH values were studied in a water/alcohol two-phase system containing Cu^2 and N-ndodecyl-L-proline(A). The influences of the solvent sort, the pH value, the concentrations of Cu^2 and chiral ligand on the partition coefficient(K) and separation factor(α) were discussed. The experimental results show that the A formed has more stable ternary complex with D-mandelic acid enantiomer than with L-mandelic acid enantiomer. There is an important influence of the pH value on K and a. When the pH values are less than 3.5, the formation of binary complexes is thermodynamically unfavourable. K and a become maximum when pH values are above 3.5 and the molar ratio of the chiral ligand to Cu^2 is 2:1.     

活性炭单柱提取肌苷新技术研究

活性炭单柱提取肌苷新技术研究李永泉，张进，姚恕，郑莲英，俞峰（杭州大学生物系杭州３１００２８）（浙江大学化工系杭州３１００２７）关键词：肌苷，提取技术，活性炭单柱１前言目前，国内肌苷发酵后处理工艺落后，在分离提取工序中肌苷损失严重，收率一般只有５０％...     

运用模式分类的雷达抗转发式距离欺骗干扰方法

针对雷达转发式欺骗干扰中的距离欺骗，提出了一种基于模式分类来识别目标与干扰的方法。该方法基于干扰和目标能量和起伏特性差异确定提取特征因子，再利用RBF神经网络方法进行分类。仿真结果表明，该方法具有较好的抗距离欺骗干扰性能。     

Application of the blind source separation method to feature extraction of machine sound signals

As the result of vibration emission in air, a machine sound signal carries important information about the working condition of machinery. But in practice, the sound signal is typically received with a very low signal-to-noise ratio. To obtain features of the original sound signal, uncorrelated sound signals must be removed and the wavelet coefficients related to fault condition must be retrieved. In this paper, the blind source separation technique is used to recover the wavelet coefficients of a monitored source from complex observed signals. Since in the proposed blind source separation (BSS) algorithms it is generally assumed that the number of sources is known, the Gerschgorin disk estimator method is introduced to determine the number of sound sources before applying the BSS method. This method can estimate the number of sound sources under non-Gaussian and non-white noise conditions. Then, the partial singular value analysis method is used to select these significant observations for BSS analysis. This method ensures that signals are separated with the smallest distortion. Afterwards, the time-frequency separation algorithm, converted to a suitable BSS algorithm for the separation of a non-stationary signal, is introduced. The transfer channel between observations and sources and the wavelet coefficients of the source signals can be blindly identified via this algorithm. The reconstructed wavelet coefficients can be used for diagnosis. Finally, the separation results obtained from the observed signals recorded in a semi-anechoic chamber demonstrate the effectiveness of the presented methods .     

Determination of gas dispersion in vapor extraction of heavy oil and bitumen

In this work, a mathematical model is developed and simulated to determine gas dispersion along with solubility during the vapor extraction (Vapex) of live oil from a laboratory scale physical model. The physical model is a rectangular block of homogenous porous medium saturated with heavy oil and bitumen. At a given temperature and pressure, the block is initially exposed on its side to a solvent gas, which diffuses into the medium and gets absorbed. The absorption of gas reduces the viscosity of heavy oil and bitumen causing it to drain under gravity. The low-viscosity “live oil” is produced at the bottom of the porous block. The production of live oil with time is accompanied by the shrinkage of oil in the block as well as its increased exposure to gas from top. These phenomena of Vapex are described by the mathematical model, which is used to calculate live oil production with various values of gas solubility and dispersion. Their optimal values are determined for the vapor extraction of Cold Lake bitumen with butane by matching calculated live oil production with its experimental values published earlier.     

Surface movements in Bologna (Po Plain — Italy) detected by multitemporal DInSAR

We studied the surface deformations affecting the southeastern sector of the Po Plain sedimentary basin, in particular the area of Bologna. To this aim an advanced DInSAR technique, referred to as DInSAR-SBAS (Small BAseline Subset), has been applied. This technique allows monitoring the temporal evolution of a deformation phenomenon, via the generation of mean deformation velocity maps and displacement time series from a data set of acquired SAR images. In particular, we have processed a set of SAR data acquired by the European Remote Sensing Satellite (ERS) sensors and compared the achieved results with optical levelling measurements, assumed as reference. The surface displacements detected by DInSAR SBAS from 1992 to 2000 are between 10 mm/year in the historical part of Bologna town, and up to 59 mm/year in the NE industrial and agricultural areas. Former measurements from optical levelling referred to 1897 show 2-3 mm/year vertical movements. This trend of displacement increased in the second half of the 20th century and the subsidence rate reached 60 mm/year. We compared the more recent levelling campaigns (in 1992 and late 1999) and DInSAR results from 1992 to 1999. The standard deviation of the difference between levelling data, projected onto the satellite Line Of Sight, and DInSAR results is 2 mm/year. This highlights a good agreement between the measurements provided by two different techniques. The explanation of soil movements based on interferometric results, ground data and geological observations, allowed confirming the anthropogenic cause (surface effect due to the overexploitation of the aquifers) and highlights a natural, tectonic, subsidence.     

正交实验优选盐生藻多糖的提取工艺

以盐生藻为原料,盐生藻多糖得率为指标,运用稀酸、稀碱和蒸馏水为提取剂提取盐生藻多糖,得出合适的提取剂为稀酸.进一步的正交实验表明,盐生藻多糖的最适提取工艺为:提取75min,pH=4,固液比为1∶70,温度为95℃.     

用SY87721实现城域网波分复用系统的多业务接入

为了使城域网的DWDM（密集波分复用）系统可以接入多种信号速率和格式，以满足用户的要求，首先介绍了一种波长转换板的设计方法，使用SY87721自适应提取28Mbit／s～2.7Gbit/s之间任意速率信号的时钟，其次介绍了SY87721提取时钟的工作原理、与单片机和CPLD（复杂可编程逻辑器件）的接口方式、对寄存器的配置和读出方式。最后介绍了使用参考时钟测量接入信号频率的方法，这会在系统维护时带来较大的方便。     

Synthesis,extraction, and adsorption properties of calix[4]arene‐poly(ethylene‐glycol) crosslinked polymer

Novel calix[4]arene‐poly(ethylene glycol) crosslinked polymer (CCP) has been synthesized by the polycondensation reaction between a p‐tert‐butylcalix[4]arene derivative and dihydroxyl capped poly(ethylene glycol) (DHPEG, M_n = 1000) catalyzed by neodymium tosylate. The hydrogel, consisted of 66.9% water and 33.1% CCP, can selectively extract aromatic organic molecules from aqueous solution according to the diameter of the guest molecules, which infers that the diameter of the calix[4]arene cavity is about 5.4 Å and the conformation of calix[4]arene units altered from cone conformation to 1,3‐alternate conformation during the polycondensation reaction. Furthermore, CCP can also adsorb naphthalene from gas phase, showing much higher capacity than active carbon does, which may have some potential applications in the field of separation and environment protection. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008