Complete and Prolonged Inhibition of Herpes Simplex Virus Type 1 Infection In Vitro by CRISPR/Cas9 and CRISPR/CasX Systems

Almost all people become infected with herpes viruses, including herpes simplex virus type 1 (HSV-1), during their lifetime. Typically, these viruses persist in a latent form that is resistant to all available antiviral medications. Under certain conditions, such as immunosuppression, the latent forms reactivate and cause disease. Moreover, strains of herpesviruses that are drug-resistant have rapidly emerged. Therefore, it is important to develop alternative methods capable of eradicating herpesvirus infections. One promising direction is the development of CRISPR/Cas systems for the therapy of herpesvirus infections. We aimed to design a CRISPR/Cas system for relatively effective long-term and safe control of HSV-1 infection. Here, we show that plasmids encoding the CRISPR/Cas9 system from Streptococcus pyogenes with a single sgRNA targeting the UL30 gene can completely suppress HSV-1 infection of the Vero cell line within 6 days and provide substantial protection within 9 days. For the first time, we show that CRISPR/CasX from Deltaproteobacteria with a single guide RNA against UL30 almost completely suppresses HSV-1 infection of the Vero cell line for 3 days and provides substantial protection for 6 days. We also found that the Cas9 protein without sgRNAs attenuates HSV-1 infection. Our results show that the developed CRISPR/Cas systems are promising therapeutic approaches to control HSV-1 infections.     

A fuzzy logic system based on Schweizer-Sklar t-norm

In recent years, the basic research of fuzzy logic and fuzzy reasoning is growing ac- tively day by day, such as the basic logic system BL proposed by Hajek[1]; fuzzy logic system MTL proposed by Esteva and Godo[2]; fuzzy reasoning, implication operators …     

异构网络解耦与耦合级联方案覆盖性能比较

针对异构网中上行链路(UL)和下行链路(DL)负载不平衡的问题,提出一种解耦UL/DL级联(DUDA)联合方案。通过双连接简化级联条件推导主、从UL接入距离的概率密度函数,利用随机几何工具推导DUDA UL覆盖概率的一般形式,从而得出整个网络的UL平均覆盖概率。数值仿真结果表明,与传统CUDA方案相比,DUDA联合方案的覆盖性能更优。     

384×288非制冷红外探测器驱动电路设计

介绍了384×288非制冷红外焦平面探测器UL03191及其工作原理,分析了非制冷红外焦平面阵列驱动电路组成原理、设计方法,重点是偏置电压电路、脉冲电压信号驱动电路、温度检测及控制电路的设计等关键技术,并对主要驱动信号进行了仿真。     

电弧故障断路器UL标准研究

介绍了UL 1699《电弧故障断路器》的主要内容及术语。UL 1699标准中核心的三大试验内容,包括电弧故障检测试验、误脱扣试验、操作抑制试验。     

UL标准电器布线进行3C认证检测分析

电器制造商希望作为配件的电器布线用电线电缆能同时通过3C和UL认证。目前针对电线电缆的检测,由于国家法律、法规和惯例的不同,国家标准(GB)和UL标准存在较大差异,难以协调一致,存在符合UL标准的电器布线不能满足3C检测的情况。本文分析UL标准电器布线进行3C检测时由于标准差异可能引起的问题。     

炼化装置消防水泵选型注意事项

消防水泵相比普通离心泵而言,有一些特殊要求,为使工程设计人员能尽快掌握消防水泵的特点,根据实际工作经验,结合 GB6245、NFPA20标准的要求,以及 FM/UL 认证的要求,总结了炼化装置中消防水泵选型时的注意事项,供工程设计人员参考使用。     

用锥形量热仪研究阻燃聚丙烯的燃烧行为

采用锥形量热仪比较了溴系阻燃聚丙烯、无卤阻燃聚丙烯的燃烧行为。结果表明:按UL94标准测试V-0阻燃等级的阻燃聚丙烯复合材料,采用无卤阻燃体系比采用溴系阻燃体系具有更低的热释放速率、烟生成速率和总烟释放量。     

浅析美国标准环境下电缆选型设计

介绍了美国电气设计标准中的AWG导线规格、电缆材料和截面积的选择以及UL电线电缆标志,结合常规电缆选型设计,整理分析了美国标准与中国标准电缆选型设计的异同,供同行业电气工程师在执行美标工程中参考。     

408UL仪器在复杂地表施工时的排列设置方法

408UL仪器先进的设计思想和灵活的应用软件为观测系统的设计带来了很大的方便；它先进的网络传输技术为野外的施工带来了极大的便利。本文讨论了408UL仪器在复杂地表地形施工中的排列设置方法。