An integrated downstream processing strategy for the recovery and partial purification of penicillin acylase from crude media

An integrated process strategy for the recovery of penicillin acylase was developed, based on precipitation of non‐enzymatic proteins directly from Escherichia coli homogenates or crude extracts using Rolquat (quaternary ammonium salt) and adsorption of the enzyme on Amp‐Seph (3.8 µmole ampicillin cm^?3) under pseudo‐affinity conditions. The effect of pH, concentrations of ammonium sulfate and Rolquat, and also concentrations of protein and cell debris on the precipitation of non‐enzymatic proteins from homogenates and crude extracts of penicillin acylase were analysed. The method of addition of Rolquat to homogenates and crude extracts significantly influenced the size of the precipitated particles. Improved results on the specific activity of penicillin acylase were obtained for 22% and 1% (w/v) of ammonium sulfate and Rolquat, respectively, added sequentially to enzyme solutions and at room temperature. Under these experimental conditions, the specific activity of penicillin acylase in homogenates and crude extracts was enhanced 2.5–3.0‐fold. Finally, the integrated process strategy was implemented first by precipitation of non‐enzymatic proteins and recovery of penicillin acylase directly from the enzyme solution treated with Rolquat using an adsorption/filtration system with an overall yield of 86%. This system allows simultaneously the filtration of cell debris and fine precipitated particles, in situ recovery of penicillin acylase by its adsorption on Amp‐Seph, and selective desorption of the enzyme with a specific activity of 11 IU (mg prot)^?1 and a desorption yield of 95%. © 2002 Society of Chemical Industry     

Kinetic investigation of the carbothermal reduction of an Iranian clay

The kinetics of the carbothermal reduction of clay under argon atmosphere has been investigated by the X-ray diffraction method. The clay-carbon (excess) mixture was formed into 2 cm diameter disks of different thicknesses. Experimental data evidences the significant effect of sample thickness on the reaction rate at 1400 °C. Decreasing thickness promotes mullite dissociation and formation of SiC and alumina powders. Mathematical modeling of the reaction system showed the gas diffusion in the Knudsen regime through the pellet to be the rate controlling step. Diffusivity of CO and reacted core tortuosity factor have been calculated.     

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本文介绍了四川盆地几十年钻井实践中遇到的主要困难。并在总结国内外碳酸盐岩油气钻井经验的基础上,针对主要困难的特点提出在四川盆地提高钻井速度的途径。     

Organic geochemistry of heavy/extra heavy oils from sidewall cores, Lower Lagunillas Member, Tia Juana Field, Maracaibo Basin, Venezuela

The study of 22 oils from sidewall cores taken at different depths in the Lower Lagunillas Member, well LSJ-AB, Tia Juana Field, Maracaibo Lake is presented, with the purpose of predicting the intervals that present the best crude oil quality. Differences were detected in the biodegradation levels of the studied samples, which are correlated with the depth at which the sidewall core was taken. The API gravity was considered for the oils from each sidewall core and it was found that toward the top of the sequence, the oils have an API gravity of 10.6-11.2°, while toward the base part of the sequence, the well produces extra heavy oils with an API gravity that varies between 8.2 and 8.7°.     

Forty-Five Years After Broadbent (1958): Still No Identification Without Attention.

According to D. E. Broadbent's (1958) selective filter theory, people do not process unattended stimuli beyond the analysis of basic physical properties. This theory was later rejected on the basis of numerous findings that people identify irrelevant (and supposedly unattended) stimuli. A careful review of this evidence, however, reveals strong reasons to doubt that these irrelevant stimuli were in fact unattended. This review exposed a clear need for new experiments with tight control over the locus of attention. The authors present 5 such experiments using a priming paradigm. When steps were taken to ensure that irrelevant stimuli were not attended, these stimuli produced no priming effects. Hence, the authors found no evidence that unattended stimuli can be identified. The results support a modern version of Broadbent's selective theory, updated to reflect recent research advances. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

数字语音处理芯片D6571E的应用

介绍了美国DSPG公司生产的数字语音处理芯片D6571E的主要特点、工作原理和引脚功能。详细说明了采用D6571E进行数字压缩录音与回放的工作原理与工作过程 ,给出了关键部分的电路设计方案。     

昆2井优快钻井及油气层保护技术

昆2井是一口位于柴北缘西段的昆特依凹陷潜伏Ⅰ号圈闭上的风险探井,设计井深为5950m。针对柴北缘深探井存在的主要技术难题,制定了昆2井的技术对策。通过对钻井设备的优选、井身结构的优化、钻头及钻具组合的优选、钻井参数及钻井液性能的优化、油气层保护技术的研究与应用,形成了一套适合于柴北缘的优快钻井技术,使昆2井的完钻井深达到5950m。与邻区的深探井平均水平相比,在井深增加888m、大尺寸井眼段更长的前提下,昆2井的钻井、完井周期分别缩短了98.75d、124.2d,钻机月速度增加了317m/台,平均机械钻速增加了0.82m/h。根据“理想充填新理论”,确定了保护油气层的最优复配暂堵方案,利用该方案所配制的钻井液其渗透率恢复值达到了87.5%以上,流变性能良好,滤失量低,对改善泥饼质量、增强井壁稳定性有明显的效果。     

Application of artificial neural networks to the analysis of two‐dimensional fluorescence spectra in recombinant E coli fermentation processes

A two‐dimensional (2D) spectrofluorometer was used to monitor various fermentation processes with recombinant E coli for the production of 5‐aminolevulinic acid (ALA). The whole fluorescence spectral data obtained during a process were analyzed using artificial neural networks, ie self‐organizing map (SOM) and feedforward backpropagation neural network (BPNN). The SOM‐based classification of the whole spectral data has made it possible to qualitatively associate some process parameters with the normalized weights and variances, and to select some useful combinations of excitation and emission wavelengths. Based on the classified fluorescence spectra a supervised BPNN algorithm was used to predict some of the process parameters. It was also shown that the BPNN models could elucidate some sections of the process's performance, eg forecasting the process's performance. Copyright © 2005 Society of Chemical Industry     

准噶尔盆地三台-北三台地区第三系勘探潜力分析

从沉积及储层组合、油气运移系统等方面全面剖析了准噶尔盆地三台-北三台地区第三系油气成藏条件，建立了成藏模式，对未钻探区勘探潜力进行了预测；通过分析，认为该区第三系储层物性主体差，断裂更多地表现为封闭性，导致沟通深部油气源的运移体系不畅，致使第三系圈闭不能有效捕获和储集油气。圈闭又比较匮乏，主体勘探潜力不大。北25井西背斜紧邻多期活动的甘诃子北断裂，第三系可能发育较好的储层，相对有利，北25井西背斜应是钻探第三系的首选目标。     

塔里木盆地塔河油区原油生物标志化合物在运移方面的应用探讨

塔河油区奥陶系原油的成熟度较高,且受生物严重降解.不同的生物标志化合物成熟度参数仅对有机质生成原油的不同阶段起到标尺作用,需优选既能较好地抗生物降解且适用于较宽的成熟度范围的指标来分析塔河油气运移的可能途径.三环萜烷/17α(H)-藿烷、重排甾烷/规则甾烷及Ts/(Ts+Tm)的比值是比较适用于塔河油区这种特殊性质的原油的生物标志化合物.根据研究,奥陶系原油油气存在两个注入通道,早期主要是由南向北方向运移,油气成熟度相对较低,成藏较早;晚期由东向西运移,原油成熟度相对于早期的较高,原油成藏稍晚.