四-(4-苯基)卟啉(TPP)分光光度法测定松花蛋中铅的含量

本实验研究了铅与非水溶性试剂四苯基卟啉(TPP)的显色反应。采取分光光度法,测定了酸碱度、温度、反应时间、共存离子等多个方面的因素对络合物的生成量及稳定性的影响,确定了反应进行的最适条件,建立起一种新型的测定食品中铅含量的分析方法。实验发现,在盐酸羟胺存在的条件下,四苯基卟啉(TPP)能与铅在碱性介质(pH12)中生成较稳定的络合物,络合比为1:1,该反应在沸水条件下只需20min即可完成。经实验测得,该络合物的最大吸收波长在466nm处。当铅含量在0～1μg/ml范围内时,测得的曲线呈线性关系,服从比尔定律。将其用于松花蛋中痕量铅的测定,结果令人满意。     

用茶叶多酚氧化酶生物传感器测定茶多酚(英文)

从鲜茶叶中提取多酚氧化酶,与氧电极偶合制成酶传感器,以焦性没食字酸子酸为底物,研究了激活剂浓度、缓冲介质、体系pH、操作温度等对响应性能的影响,测得对茶多酚响应的酶动力学方程为R(mV/S)=17.35C/(3.058+C)(C:g/L),米氏常数为3.06g/L,响应的线性范围为1.5～9.4g/L,用于茶叶样品与茶多酚制品中茶多酚含量的测定,结果与国家标准方法的相符。从而为茶多酚含量的测定建立了一种生物传感新方法。     

邻苯二甲酸催化合成四苯基卟啉

报道了以邻苯二甲酸为催化剂催化合成四苯基卟啉的工艺。实验表明,对于0.02 mol的吡咯,最佳的溶剂为二甲苯,物料比为n(吡咯)∶n(苯甲醛)=1∶1,催化剂邻苯二甲酸的用量为0.008 mol,反应时间为4 h,得到四苯基卟啉的产率为29.9%。相比其他催化剂,反应中棕黑色副产物少,分离纯化方便,通过无水乙醇和丙酮重结晶,纯度可达98.7%(HPLC)。四苯基卟啉用IR、UV、1HNMR验证了结构。     

Does Japan’s accession to the Trans-Pacific Partnership mean an opportunity or a threat to the US textile industry? A quantitative evaluation

This study provides a quantitative evaluation on the potential impact of the implementation of the Trans-Pacific Partnership (TPP) on the economic interests in the US textile industry, particularly with regard to Japan’s accession to the agreement. By adopting the Global Trade Analysis Project (GTAP) computable general equilibrium model based on the GTAP8 database, results of the study show that: first, trade creation effect of the TPP would result in an increase of the US textile exports to other TPP members; however, with Japan’s accession to the TPP, the potential expansion of the US textile exports would be substantially offset. Second, the TPP would negatively affect the US textile exports to the North American Free Trade Agreement and CAFTA regions, particularly in the case when Japan becomes a TPP member. Third, the TPP would expand the two-way intra-industry trade between the United States and Japan for textiles. Fourth, the TPP would cause an expansion of the trade deficit as well as a decline of domestic production in the US textile industry, particularly in the case when Japan becomes a TPP member. Findings of this study augment our understanding of the Textile and apparel (T&A)-specific sectoral impact of the TPP and provide valuable inputs supporting the T&A sectoral negotiation under the TPP.     

Novel carboxymethyl derivatives of chitin and chitosan materials and their biomedical applications

Chitin and chitosan are natural biopolymers that are non-toxic, biodegradable and biocompatible. In the last decade, chitin and chitosan derivatives have garnered significant interest in the biomedical and biopharmaceutical research fields with applications as biomaterials for tissue engineering and wound healing and as excipients for drug delivery. Introducing small chemical groups to the chitin or chitosan structure, such as alkyl or carboxymethyl groups, can drastically increase the solubility of chitin and chitosan at neutral and alkaline pH values without affecting their characteristics; substitution with carboxyl groups can yield polymers with polyampholytic properties. Carboxymethyl derivatives of chitin and chitosan have shown promise for adsorbing metal ions, as drug delivery systems, in wound healing, as anti-microbial agents, in tissue engineering, as components in cosmetics and food and for anti-tumor activities. This review will focus on the preparative methods and applications of carboxymethyl and succinyl derivatives of chitin and chitosan with particular emphasis on their uses as materials for biomedical applications.     

Small Molecule Arranged Thermal Proximity Coaggregation (smarTPCA)—A Novel Approach to Characterize Protein–Protein Interactions in Living Cells by Similar Isothermal Dose–Responses

Chemical biology and the application of small molecules has proven to be a potent perturbation strategy, especially for the functional elucidation of proteins, their networks, and regulators. In recent years, the cellular thermal shift assay (CETSA) and its proteome-wide extension, thermal proteome profiling (TPP), have proven to be effective tools for identifying interactions of small molecules with their target proteins, as well as off-targets in living cells. Here, we asked the question whether isothermal dose–response (ITDR) CETSA can be exploited to characterize secondary effects downstream of the primary binding event, such as changes in post-translational modifications or protein–protein interactions (PPI). By applying ITDR-CETSA to MAPK14 kinase inhibitor treatment of living HL-60 cells, we found similar dose–responses for the direct inhibitor target and its known interaction partners MAPKAPK2 and MAPKAPK3. Extension of the dose–response similarity comparison to the proteome wide level using TPP with compound concentration range (TPP-CCR) revealed not only the known MAPK14 interaction partners MAPKAPK2 and MAPKAPK3, but also the potentially new intracellular interaction partner MYLK. We are confident that dose-dependent small molecule treatment in combination with ITDR-CETSA or TPP-CCR similarity assessment will not only allow discrimination between primary and secondary effects, but will also provide a novel method to study PPI in living cells without perturbation by protein modification, which we named “small molecule arranged thermal proximity coaggregation” (smarTPCA).     

TPP与KORUS FTA和NAFTA的原产地规则对比分析

原产地规则是自由贸易协定中的重要内容,由于其所具有的判断货物"法定国籍"的特定角色,所以它在一定程度上决定了自由贸易区的自由程度。本文将先总体介绍原产地规则的相关知识,然后选取TPP中的原产地规则中的几点,分别与NAFTA和KORUE FTA进行对比分析。     

The study of coupling effects of humidity-heat on the protection performance of protective clothing for fire fighting

The coupling effects of humidity-heat on the thermal protective performance of protective clothing play an important role during the fire fighting process. The thermal stability, heat release, and thermal protective performance of protective clothing were investigated under the coupling effects of humidity-heat condition. The cone calorimeter and the thermogravimetric analysis (hereinafter referred to as TGA) were adopted to investigate the thermal stability and heat release characteristics of protective clothing. The thermal protective performance tester and heat flux meter 8 (hereinafter referred to as HFM-8) multichannel heat flow meter were adopted to simulate the multiple effects of internal sweating, external water spray and high thermal radiation. As for the thermal protective performance experiment (hereinafter referred to as TPP) results, it is found that the proper moisture content can significantly enhance the thermal protective performance, but the performance is seriously degraded after soaking, which is due to the improved thermal conductivity. The mechanism of microscopic changes of protective clothing were also investigated by hot-stage polarizing microscope and scanning electron microscope. The results of this experiment have important practical guiding significance for the safety protection of firefighters during the fire extinguishing processes.     

Antioxidant and antimicrobial activities of consecutive extracts from Galla chinensis:The polarity affects the bioactivities

The gallotannins of Galla chinensis (GAC) were extracted consecutively by five solvents of different polarity, and the antioxidant and antimicrobial activities of the extracts were evaluated. All of the ether-, ethyl acetate-, ethanol-, and water-extracts presented remarkable antioxidant abilities in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power (FRAP), β-carotene linoleic acid system, and hydroxyl radical scavenging assays. The minimum inhibitory concentrations (MICs) of these extracts against 3 species of gram-positive bacteria, 3 species of gram-negative bacteria, and 3 species of fungi were also determined using the agar dilution method. All of the extracts showed excellent antibacterial activities, but no antifungal activities. The gallotannins in different extracts were analyzed by the HPLC and HPLC-ESI-MS. The results indicated that the extracts with weaker polarity contained gallotannins with higher molecular weight, and had stronger antioxidant and antibacterial activities.