Clinically Applicable Assessment of Tisagenlecleucel CAR T Cell Treatment by Digital Droplet PCR for Copy Number Variant Assessment

Chimeric antigen receptor (CAR) T cell therapy is an innovative immunotherapy for treating cancers in both children and adults with proven utility in numerous clinical trials. Significantly, some CAR T cell therapies have now been approved by relevant national regulatory bodies across numerous countries for clinical therapeutic use outside of clinical trials. One such recently licensed product is tisagenlecleucel, a CAR T therapy approved for the treatment of B-cell acute lymphoblastic leukemia (B-ALL) using autologous T cells from the patient. The genetically engineered T cells target a protein called CD19, common to B cells, through a CAR incorporating a 4-1BB costimulatory domain to improve response. Since tisagenlecleucel is now a standard of care treatment for B-ALL, it is clinically essential to be able to accurately monitor these CAR T cells in patients. Assessment of the copy number variant (CNV) of the CAR T cell products allows this within a clinically acceptable timeframe for optimal patient benefit. However, no standardized method with high reproducibility and efficiency has been described within a routine clinical laboratory setting. Here, we demonstrated a novel digital droplet PCR (ddPCR)-based methodology for the study of CNV (ddPCR-CNV) in 4-1BB CD19-specific CAR T cells with universal applicability across clinical diagnostic laboratories.     

基于免疫的动态垃圾邮件过滤研究

本文基于生物免疫系统防御机制,探讨了将人工免疫原理应用于垃圾邮件过滤系统(IDSFS),通过动态生成垃圾邮件疫苗,提高系统的检出率.另外,针对传统邮件过滤系统存在的错误肯定率、错误否定率等不足及用户对邮件个性化处理的要求,引入用户反馈机制,使得系统精确率、正确率进一步提高.实验结果表明,该系统具有较强的自学习、自适应能力.     

未来弹性与AES

介绍了未来弹性的概念,对将未来弹性引入AES评估标准的优点进行了探讨,以来来弹性的观点分析了第二轮AES候选算法的遴选并提出了具体建议。     

一种用于核辐射监测信号的多微处理系统

介绍了4兆伏静电正离子加速器的监测防护系统,该系统由多个微处理器与主控机分布式分布,组成高速多通道辐射信号采集控制系统,可以对多点核辐射的能量、强度、剂量率,剂量及剂量当量进行监测.系统的核电路部分采用最新的芯片产品,合理的硬件安排,优化的软件编程,在仅更换不同的探头后,便可探测分析γ,X及快中子辐射.在参数稍做调整之后,就可以广泛应用于类似条件的其他场所的辐射监测.     

新型芳香二胺的合成及其聚酰亚胺的应用研究

首先通过双酚A和碳酸钾反应得到酚钾盐,与间-二硝基苯反应合成2,2-双[4-(3-硝基苯氧基)苯基]丙烷(3-BNPP),再用水合肼还原得到新型芳香二胺单体2,2-双[4-(3-氨基苯氧基)苯基]丙烷(3-BAPP)。用3-BAPP与多种二酐合成出多种聚酰亚胺,并在二层法挠性覆铜板(2L-FCCL)的应用方面做了一些研究。研究表明其相应的2L-FCCL具有良好的耐锡焊性能和较低的吸水率。     

Downregulation of miR-122-5p Activates Glycolysis via PKM2 in Kupffer Cells of Rat and Mouse Models of Non-Alcoholic Steatohepatitis

Non-alcoholic steatohepatitis (NASH) has pathological characteristics similar to those of alcoholic hepatitis, despite the absence of a drinking history. The greatest threat associated with NASH is its progression to cirrhosis and hepatocellular carcinoma. The pathophysiology of NASH is not fully understood to date. In this study, we investigated the pathophysiology of NASH from the perspective of glycolysis and the Warburg effect, with a particular focus on microRNA regulation in liver-specific macrophages, also known as Kupffer cells. We established NASH rat and mouse models and evaluated various parameters including the liver-to-body weight ratio, blood indexes, and histopathology. A quantitative phosphoproteomic analysis of the NASH rat model livers revealed the activation of glycolysis. Western blotting and immunohistochemistry results indicated that the expression of pyruvate kinase muscle 2 (PKM2), a rate-limiting enzyme of glycolysis, was upregulated in the liver tissues of both NASH models. Moreover, increases in PKM2 and p-PKM2 were observed in the early phase of NASH. These observations were partially induced by the downregulation of microRNA122-5p (miR-122-5p) and occurred particularly in the Kupffer cells. Our results suggest that the activation of glycolysis in Kupffer cells during NASH was partially induced by the upregulation of PKM2 via miR-122-5p suppression.     

Assessing the reliability of the M5-120 on Amazon’s mechanical Turk

Amazon’s online service, Mechanical Turk (MTurk) has become a popular option for data collection among social scientists. Early work (Buhrmester, Kwang, & Gosling, 2011) indicated that data collection through MTurk was faster and less expensive than traditional collection methods (undergraduate human subject pool), as well as being reliable when administered at different dates. Building on their work, we sought to extend this investigation of reliability to a larger measure. For the current research we chose a 120-item measure of personality. After collecting data through MTurk, it was determined that our MTurk sample had strong test–retest reliability, indicating that they did not significantly change between administration dates.     

CHINALCO Seek Rare Earth Project in Guangdong

【正】CHINALCO and the Qingyuan municipal government in Guangdong will sign a framework agreement at the beginning of 2011 to jointly tap local rare earth resource...     

短程硝化反硝化生物脱氮技术的探讨

短程硝化反硝化技术是将硝化反应控制在亚硝酸盐阶段,不进行亚硝酸盐至硝酸盐的转化,直接进行反硝化反应。文章阐述了短程硝化反硝化的形成机理,理论研究进展,系统介绍了短程硝化反硝化影响因素与控制分析,并探讨了短程硝化反硝化生物脱氮技术需深入研究的要点     

3-丙酰氨基-N,N-二丙酸甲酯基苯胺的合成研究

用无水三氯化铝作催化剂在较低温度下将3-氨基丙酰苯胺和丙烯酸甲酯转化为3-丙酰氨基-N,N-二丙酸甲酯基苯胺,其产品收率达到89%,纯度高于92%。