A method for quantifying cell size from differential interference contrast images: validation and application to osmotically stressed chondrocytes

An automatic image analysis method was developed to determine the shape and size of spheroidal cells from a time series of differential interference contrast (DIC) images. The program incorporates an edge detection algorithm and dynamic programming for edge linking. To assess the accuracy and working range of the method, results from DIC images of different focal planes and resolutions were compared to confocal images in which the cell membrane was fluorescently labelled. The results indicate that a 1‐µm focal drift from the in‐focus plane can lead to an overestimation of cell volume up to 14.1%, mostly due to shadowing effects of DIC microscopy. DIC images allow for accurate measurements when the focal plane lies in a zone slightly above the centre of a spherical cell. In this range the method performs with 1.9% overall volume error without taking into account the error introduced by the representation of the cell as a sphere. As a test case, the method was applied to quantify volume changes due to acute changes of osmotic stress.     

fsh—A functional unix command interpreter

Command interpreters under the UNIX operating system typically employ the syntax and semantics of imperative programming languages. Many of the advantages that functional programming languages hold over imperative languages can be incorporated in a functional command interpreter. Unfortunately, functional command interpreters to date have used the syntax of Backus's FP. The advantages of command expressiveness and composition have not been appreciated. A powerful functional command interpreter, fsh, that overcomes many of the deterrents of previous functional command interpreters is presented. Much of the unnecessary complexity of existing UNIX command interpreters is avoided by following the functional paradigm. fsh has been implemented under the UNIX 4.2BSD and System V operating systems.     

Using linear models in optimizing parameters of time-triggered protocols

A mechanism of synchronization of components of distributed systems in which communications are based on a common time schedule for all system components is illustrated by examples of simple Time-Triggered Protocols. For parametric linear models of synchronization, the optimization problem is considered for a parameter that should exclude the overlapping of messages in communication channels. The results of using numeric methods for estimation of the optimal value of this parameter are described. __________ Translated from Kibernetika i Sistemnyi Analiz, No. 3, pp. 24–32, May–June 2006.     

Identification environment and robust forecasting for nonlinear time series

In this paper, the methods of time series for nonlinearity are briefly surveyed, with particular attention paid to a new test design based on a neural network specification. The proposed integrated expert system contains two main components: an identification environment and a robust forecasting design. The identification environment can be viewed as a integrated dynamic design in which cognitive capabilities arise as a direct consequence of their self-organizational properties. The integrated framework used for discussing the similarities and differences in the nonlinear time series behavior is presented. Moreover, its performance in prediction proves to be superior than the former work. For the investigation of robust forecasting, we perform a simulation study to demonstrate the applicability and the forecasting performance.     

A globally stable discrete-time controller for current-fed induction motors

In this short paper we present a discrete-time field oriented controller (FOC) for current-fed induction motors which insures global asymptotic speed regulation as well as rotor flux norm tracking. To the best of our knowledge, this is the first time such a result is rigorously established for a controller implemented in discrete-time. To insure global stability a condition on the reference for the rotor flux norm, which is time-varying, is imposed. This condition disappears as the sampling period goes to zero, hence allowing for independent speed regulation and rotor flux norm tracking. One important feature of our scheme is that, compared with the first-difference approximation of the classical indirect FOC, the additional computational burden is negligible. It is also shown that the result can easily be extended to the case of tracking time-varying references in speed or position.     

用面向对象编程实现问题求解自动化

本文从面向对象方法基本原理和面向对象编程的技术特征出发，阐明所建应用软件系统中的对象，类及相应类层次结构和类组合结构，使系统具有很强的表现真空世界复杂系统结构的能力，系统通过消息传递在程序执行中实现对操作的调用机制，使之在面向用户问题选择和执行求解策略方面有很强的适应性，这一点对于强调人－机交互和解题协作的智能决策支持系统设计至关重要，文章给出了用Ｃ＋＋实现的模型对象系统及消息传递机制。     

Identification of delayed system using instrumental variable method

System identification uses system inputs and outputs to raise mathematical models.Various techniques of system identification exist that offer a nominal model and an uncertainty bound.Many practical systems such as thermal processes & chemical processes have inbuilt time delay.If the time delay used in the system model for controller design does not concur with the actual process time delay,a closed-loop system may be unstable or demonstrate unacceptable transient response characteristics so here the time delay is assumed to be time-invariant. This paper proposes on-line identification of delayed complex/uncertain systems using instrumental variable(Ⅳ) method.Parametric uncertainty has been considered which may be represented by variations of certain system parameters over some possible range.This method allows consistent estimation when the system parameters are associated with the noise terms,as the IV methods(IVM’s)usually make no assumption on the noise correlation configuration.The faster convergence of the parameters including noise terms has been proved in this paper.Iterative prefiltering(IP)method has also been used for the identification of the delayed uncertain system and the graphical results given in this paper demonstrate that the convergence results are inferior to the instrumental variable method.     

基于Matlab的线性规划问题求解

线性规划是运筹学中研究较早、发展较快、应用广泛、方法成熟的一个重要分支,它是辅助人们进行科学管理的一种重要的数学方法.文章首先介绍了线性规划的基本概念及标准形式,着重讨论了线性规划问题的三种常用解法:单纯形法、直接搜索法以及遗传算法,最后在Matlab R2009a环境下进行了仿真.通过结果可以看出,用Matlab求解线性规划问题,可以避免手工的烦琐计算,大大地提高工作效率和结果的准确性.     

基于多模型半参数非线性回归模型的外弹道节省参数估计

双星定位系统的应用是外弹道估计中的一种发展趋势。文章给出基于双星、光雷的联合测量模型,建立基于半参数回归的外弹道节省参数估计模型;给出基于半参数回归的外弹道节省参数估计算法及计算步骤,并从理论上分析了基于半参数回归的外弹道节省参数估计算法能有效地消除非线性因素对参数估计性能的影响,其估计精度优于经典的节省参数建模方法,并通过仿真得到：该算法确实有效。     

Adaptive fuzzy decentralized control for nonlinear large-scale systems based on high-gain observer

An adaptive fuzzy decentralized backstepping output-feedback control approach is proposed for a class of nonlinear large-scale systems with completely unknown functions,the interconnections mismatched in control inputs,and without the measurements of the states.Fuzzy logic systems are employed to approximate the unknown nonlinear functions,and an adaptive high-gain observer is developed to estimate the unmeasured states.Using the designed high-gain observer,and combining the fuzzy adaptive control theory with backstepping approach,an adaptive fuzzy decentralized backstepping output-feedback control scheme is developed.It is proved that the proposed control approach can guarantee that all the signals of the closed-loop system are semi-globally uniformly ultimately bounded(SUUB),and that the observer errors and the tracking errors converge to a small neighborhood of the origin by appropriate choice of the design parameters.Finally,a simulation example is provided to show the eectiveness of the proposed approach.