Dipotassium Glycyrrhizinate on Melanoma Cell Line: Inhibition of Cerebral Metastases Formation by Targeting NF-kB Genes-Mediating MicroRNA-4443 and MicroRNA-3620—Dipotassium Glycyrrhizinate Effect on Melanoma

Glycyrrhizic acid (GA), a natural compound isolated from licorice (Glycyrrhiza glabra), has exhibited anti-inflammatory and anti-tumor effects in vitro. Dipotassium glycyrrhizinate (DPG), a dipotassium salt of GA, also has shown an anti-tumor effect on glioblastoma cell lines, U87MG and T98G. The study investigated the DPG effects in the melanoma cell line (SK-MEL-28). MTT assay demonstrated that the viability of the cells was significantly decreased in a time- and dose-dependent manner after DPG (IC₅₀ = 36 mM; 24 h). DNA fragmentation suggested that DPG (IC₅₀) induced cellular apoptosis, which was confirmed by a significant number of TUNEL-positive cells (p-value = 0.048) and by PARP-1 [0.55 vs. 1.02 arbitrary units (AUs), p-value = 0.001], BAX (1.91 vs. 1.05 AUs, p-value = 0.09), and BCL-2 (0.51 vs. 1.07 AUs, p-value = 0.0018) mRNA compared to control cells. The proliferation and wound-healing assays showed an anti-proliferative effect on DPG-IC₅₀-treated cells, also indicating an inhibitory effect on cell migration (p-values < 0.001). Moreover, it was observed that DPG promoted a 100% reduction in melanospheres formation (p-value = 0.008). Our previous microRNAs (miRs) global analysis has revealed that DPG might increase miR-4443 and miR-3620 expression levels. Thus, qPCR showed that after DPG treatment, SK-MEL-28 cells presented significantly high miR-4443 (1.77 vs. 1.04 AUs, p-value = 0.02) and miR-3620 (2.30 vs. 1.00 AUs, p-value = 0.01) expression compared to control cells, which are predicted to target the NF-kB, CD209 and TNC genes, respectively. Both genes are responsible for cell attachment and migration, and qPCR revealed significantly decreased CD209 (1.01 vs. 0.54 AUs, p-value = 0.018) and TNC (1.00 vs. 0.31 AUs, p-value = 2.38 × 10⁻⁶) mRNA expression levels after DPG compared to untreated cells. Furthermore, the migration of SK-MEL-28 cells stimulated by 12-O-tetradecanoylphorbol-13-acetate (TPA) was attenuated by adding DPG by wound-healing assay (48 h: p-value = 0.004; 72 h: p-value = 7.0 × 10⁻⁴). In addition, the MMP-9 expression level was inhibited by DPG in melanoma cells stimulated by TPA and compared to TPA-treated cells (3.56 vs. 0.99 AUs, p-value = 0.0016) after 24 h of treatment. Our results suggested that DPG has an apoptotic, anti-proliferative, and anti-migratory effect on SK-MEL-28 cells. DPG was also able to inhibit cancer stem-like cells that may cause cerebral tumor formation.     

Systematic Review: Targeted Molecular Imaging of Angiogenesis and Its Mediators in Rheumatoid Arthritis

Extensive angiogenesis is a characteristic feature in the synovial tissue of rheumatoid arthritis (RA) from a very early stage of the disease onward and constitutes a crucial event for the development of the proliferative synovium. This process is markedly intensified in patients with prolonged disease duration, high disease activity, disease severity, and significant inflammatory cell infiltration. Angiogenesis is therefore an interesting target for the development of new therapeutic approaches as well as disease monitoring strategies in RA. To this end, nuclear imaging modalities represent valuable non-invasive tools that can selectively target molecular markers of angiogenesis and accurately and quantitatively track molecular changes in multiple joints simultaneously. This systematic review summarizes the imaging markers used for single photon emission computed tomography (SPECT) and/or positron emission tomography (PET) approaches, targeting pathways and mediators involved in synovial neo-angiogenesis in RA.     

基于相位过渡过程的WI语音重建方法

通过研究语音残差信号的合成方法,给出了一种改进的波形内插（Waveform Interpolation,WI）编码器语音重建方案,去除了解码器中特征波形（Characteristic waveform,CW）的对齐运算。在该方案中当帧间的基音周期连续变化时,语音残差信号由幅度谱和相位轨迹直接合成,而当基音周期发生跳变时,则利用相位过渡过程合成语音残差信号。该方法大大降低了WI解码器的复杂度,同时保证了合成语音质量没有变化。     

混合式步进电动机的矢量控制分析

混合式步进电动机与传统的异步、同步电动机相比,由于定子磁势、转子磁钢的非线性分布以及磁饱和等因素的存在而没有简单的线性化数学模型.根据已有数学模型,利用电动机矢量控制原理,在d-q坐标系下推导出混合式步进电动机电磁转矩与电流关系、静态控制与动态控制的数学模型,以及电动机定子电流细分算法,最后通过搭建实验样机验证其控制方法的有效性.     

FNN与混合遗传算法在智能优化的策略比较

FNN是一种重要的神经网络结构,混合遗传算法是一种正在兴起的启发式随机搜索技术,两者在智能优化中得到了广泛的应用。两者的优化机理不同,方法不同,各有千秋。论文从提高两者的优化性能出发,研究其智能优化的实现策略,并对两者进行了比较,得出了有意义的结论。     

一类非线性系统最大可控不变集求解

针对非线性系统线性化在状态约束下最优鲁棒控制求解问题,提出了一种基于混合系统的非线性系统最大鲁棒控制不变集的方法.对于一类非线性系统通过平衡点线性化的方法转化为多模态的混合系统,并进行了混合逻辑动态模型(MLD)的建模,在不变集基本理论的基础上,通过多参数规划的混合整数规划(MIQP)的方法迭代求解最大可控不变集,并求得不变集内的最优控制器,解决系统的状态约束问题.通过一个非线性系统的实例进行建模、仿真,证明了本方法的可行性.     

改进混合量子算法在Job Shop调度中的研究

自从科学的管理思想引进生产过程中,管理者开始注重计算机的辅助作用。Job Shop调度问题（JSP）是许多实际生产调度的简化模型,由于解空间的规模巨大,给求解带来了很大的挑战。在量子比特编码的基础上,设计了两种解码方式,结合微粒群算法（PSO）的更新式对量子角进行智能调整,形成了混合量子算法（HQA）。但HQA在求解JSP时,效果并不好。在HQA的框架下,增加了一些寻优机制,使得算法求解性能有显著的提高,并称其为改进混合量子算法（IHQA）。     

基于间接矩阵变换器的异步电动机直接转矩控制

间接矩阵变换器是一种直接变换型交流-交流电力变换装置,与交-直-交变换器和传统矩阵变换器相比,具有很多优良的特性。异步电动机直接转矩控制以其简洁明了的系统结构,优良的静、动态性能,获得转矩的高动态性能。为了在实现异步电动机的高动静态性能调速的同时,满足电网电能质量的要求,提出一种采用间接矩阵变换器驱动异步电动机的直接转矩控制系统调制策略。利用这种调制策略,在异步电动机高速运行的条件下,一方面实现异步电动机的高性能交流调速,另一方面使系统具有良好的输入特性,能够满足日益严格的电网电能质量的要求。数值仿真结果验证这种调制策略的可行性。     

旋转部件非接触式测温装置供电及通信研究

针对高速辊体温度非接触式测量装置供电及通信单元结构复杂、易受干扰的问题,研制了一种结构简单、测量精度高的新型测温装置.利用由两个电感线圈构成的空心变压器既完成能量传送又能进行信息通信.定子侧直流电源经全桥逆变后利用电感耦合原理向转子供电.通过曼彻斯特编码方式调制采样数据得到通信信号,利用反向调制原理,向定子侧传递温度信息完成定转子之间通信.经理论计算与硬件实验表明,装置具有良好的供电稳定性以及通信抗干扰能力.     

C/纳米[Ni0.96Co0.04(OH)2-C]混合超级电容器

以纳米Ni0.96Co0.04(OH)2-C复合材料[Ni0.96Co0.04(OH)2(90%)+FJC(10%)]为正极,活性炭为负极,KOH水溶液为电解液组成混合超级电容器.其工作电压为0.75～1.5 V;适合的充电方法是恒流恒压充电;具有快速充电、高倍率放电和循环性良好的特点.8min可充满80%容量,2 200W/kg(以正、负极活性物总质量计算)放电时,比能量达23.2Wh/kg.