光学式颗粒计数装置的研制

设计了一种检测装置,用来对洁净介质中的颗粒粒径大小和数量进行统计。详细分析了用几何光学的光阻公式替代消光公式对大粒径颗粒的检测机理和前向光散射探测器检测小粒径颗粒时的计数技术。用该装置对标准颗粒物质进行了检测实验,结果表明：对于2μm以上的颗粒计数。效率能达到90％;对于1μm以下的颗粒也有较好的分辨能力,计数效率在70％左右。数值计算和实验结果证实了该装置具有较好的计数能力,能够表征洁净介质的污染程度。     

生物超微弱发光的光子计数成象方法

介绍了我们研制和设计的用于测量生物超微弱发光的光子计数成象系统，包括光子成象头，高帧频ＣＣＤ摄象机，图象采集系统和计算机，可以定量地探测出生物的发光强度分布 ，还能够显示出其发光的图象，确定发光的形状和位置，本系统的信噪比为１０：１，最小探测功率达１０＾－１６Ｗ，灵敏度高，动态范围大。     

一种皮秒单光子计数光谱仪数据采集系统

针对皮秒时间相关单光子计数光谱仪中多道分析仪传输速率不高的情况,在荧光光谱测量中的多道定标技术的基础上,提出了采用 PCI 总线的数据采集卡改进方案。该方案以存储器中不同的地址作为测量中不同的道,将以 FPGA 实现的高速计数器按选定时间间隔的计数结果存入相应道中,存储器中数据经 PCI 总线读入内存并分析处理,从而取代原系统中的多道分析仪。实验结果显示,数据采集卡传输速率达到 20MB/s,比多道分析仪提高了 200 倍。     

MODES: A FORTRAN IV program to calculate modal analyses from raw point-count data

Program MODES is a FORTRAN IV program to calculate modal analyses of thin sections from raw data obtained by any normal point-counting technique. The program provides a table listing the modal analyses, quartz-feldspar-rock fragments (QFR) ratios, matrix-labiles-quartz (MLQ) ratios, ratios of plagioclase to total feldspar (P/F), ratios of volcanic lithic fragments to total lithic fragments (V/L), frequency, estimate, and confidence limits for each sample, and calculates the means and standard deviations for each component in a group of samples. It also prints a triangular diagram of the QFR or MLQ ratios. The program is useful where large numbers of modal analyses are determined because it requires no manual calculations, but it also has been determined useful when only a few samples are involved.     

Multichannel software event counter for the MC6809

Software can provide a cheap means of counting low-frequency events such as egg laying. A counting technique and driver program for this example are described.     

μC/OS-Ⅱ实时操作系统中时间管理的改进

通过介绍μC/OS-Ⅱ实时操作系统中的时间管理功能,分析了系统中任务延时的优缺点.研究了一种基于硬件的延时机制,以提高系统的运行速度与实时性.在C8051F120单片机上进行测试,经实验得出此方法可以提高系统的实时性,减少系统的额外开销.     

基于PDA的血细胞自动计数系统的研究

为克服现有血细胞计数仪结构复杂、体积大的缺点,介绍了一种基于个人数字助理(PDA)的便携式血细胞自动计数系统.本系统具有操作简便、体积小、准确度高等优点,能够满足野外血细胞计数检验的需要.系统包括显微镜、CCD摄像头、PDA,并采用eVC++4.0编程,实现了WIN CE操作系统下细胞图片的实时观察与处理,直接获得细胞...     

基于乘客多运动行为的公交客流计数判定方法

针对基于单目视觉的公交乘客人数统计判定方法不稳定、计数结果不准确的现状,结合公交车门附近乘客运动行为的复杂性和多样性,以及乘客运动行为对计数判定方法的干扰,给出一种基于乘客多运动行为分析的计数判定方法。采用轨迹聚类的方式对乘客运动行为进行分析,结合轨迹的空间特征和方向特征计算轨迹距离,并使用层次聚类方法进行聚类。分析聚类结果中每一类别所对应乘客类的运动行为,讨论各乘客类的运动行为对常用计数判定准则的影响,由此提出一种改进的公交车客流计数判定方法。利用采集的乘客上下公交车视频图像进行实验,结果表明,该方法能获得较高的统计精度和较好的稳定性。     

TimeLapseAnalyzer: multi-target analysis for live-cell imaging and time-lapse microscopy

The direct observation of cells over time using time-lapse microscopy can provide deep insights into many important biological processes. Reliable analyses of motility, proliferation, invasive potential or mortality of cells are essential to many studies involving live cell imaging and can aid in biomarker discovery and diagnostic decisions. Given the vast amount of image- and time-series data produced by modern microscopes, automated analysis is a key feature to capitalize the potential of time-lapse imaging devices. To provide fast and reproducible analyses of multiple aspects of cell behaviour, we developed TimeLapseAnalyzer. Apart from general purpose image enhancements and segmentation procedures, this extensible, self-contained, modular cross-platform package provides dedicated modalities for fast and reliable analysis of multi-target cell tracking, scratch wound healing analysis, cell counting and tube formation analysis in high throughput screening of live-cell experiments. TimeLapseAnalyzer is freely available (MATLAB, Open Source) at http://www.informatik.uni-ulm.de/ni/mitarbeiter/HKestler/tla.     

Texture analysis of poly-adenylated mRNA staining following global brain ischemia and reperfusion

Texture analysis provides a means to quantify complex changes in microscope images. We previously showed that cytoplasmic poly-adenylated mRNAs form mRNA granules in post-ischemic neurons and that these granules correlated with protein synthesis inhibition and hence cell death. Here we utilized the texture analysis software MaZda to quantify mRNA granules in photomicrographs of the pyramidal cell layer of rat hippocampal region CA3 around 1 h of reperfusion after 10 min of normothermic global cerebral ischemia. At 1 h reperfusion, we observed variations in the texture of mRNA granules amongst samples that were readily quantified by texture analysis. Individual sample variation was consistent with the interpretation that animal-to-animal variations in mRNA granules reflected the time-course of mRNA granule formation. We also used texture analysis to quantify the effect of cycloheximide, given either before or after brain ischemia, on mRNA granules. If administered before ischemia, cycloheximide inhibited mRNA granule formation, but if administered after ischemia did not prevent mRNA granulation, indicating mRNA granule formation is dependent on dissociation of polysomes. We conclude that texture analysis is an effective means for quantifying the complex morphological changes induced in neurons by brain ischemia and reperfusion.