Analysis of the Structure and Biosynthesis of the Lipopolysaccharide Core Oligosaccharide of Pseudomonas syringae pv. tomato DC3000

Lipopolysaccharide (LPS), the major component of the outer membrane of Gram-negative bacteria, is important for bacterial viability in general and host–pathogen interactions in particular. Negative charges at its core oligosaccharide (core-OS) contribute to membrane integrity through bridging interactions with divalent cations. The molecular structure and synthesis of the core-OS have been resolved in various bacteria including the mammalian pathogen Pseudomonas aeruginosa. A few core-OS structures of plant-associated Pseudomonas strains have been solved to date, but the genetic components of the underlying biosynthesis remained unclear. We conducted a comparative genome analysis of the core-OS gene cluster in Pseudomonas syringae pv. tomato (Pst) DC3000, a widely used model pathogen in plant–microbe interactions, within the P. syringae species complex and to other plant-associated Pseudomonas strains. Our results suggest a genetic and structural conservation of the inner core-OS but variation in outer core-OS composition within the P. syringae species complex. Structural analysis of the core-OS of Pst DC3000 shows an uncommonly high phosphorylation and presence of an O-acetylated sugar. Finally, we combined the results of our genomic survey with available structure information to estimate the core-OS composition of other Pseudomonas species.     

焊接箱型截面构件在不同焊缝熔深下承载力性能试验研究

为了有效改善大跨度桥梁中管翼缘焊接箱型截面构件的耐久性的问题,提出采用耐候钢代替普通钢的管翼缘箱型截面梁。同时,为了研究耐候钢管翼缘焊接箱型截面构件填充混凝土后在全熔透和部分熔透条件下的单轴承载力性能,开展了焊接矩形截面在三种不同焊缝熔深下的耐候钢管混凝土截面轴压短柱试验研究。分别测试每个试件在单轴压力作用下的静态应变、动态应变,以及竖向位移和侧向位移。对比构件承载力的试验结果及理论分析结果,得出不同熔深下焊接箱型截面管翼缘组合构件的承载力性能。研究结果表明:1）耐候钢管翼缘焊接箱型截面构件的全熔透焊缝承载力高于部分熔透焊缝承载力。2）全熔透试件开裂时的竖向位移较部分熔透试件的位移值大,而不同熔深下构件的横向位移测点L4随轴压荷载的增大而有增加,L3测点横向位移规律不明显。3）不同耐候钢管翼缘焊接箱型截面构件横向整体受拉,局部在顶部角部位置受压。构件的混凝土内部和钢板表面应变情况复杂,规律不明显。     

高强度结构钢的韧性启裂和稳态扩展

研究了具有一定韧度的高强度结构钢裂纹早期扩展阻力与材料的微观组织结构、应力状态、变形历史和应变特性之间的关系。结果表明:裂纹扩展阻力(指J_R阻力曲线中的J_i和dJ/da)主要决定于裂纹尖端的塑性约束程度和应力应变场中消耗的弹性能和塑性功。这与三轴应力状态函数σ_mσ~(1/2)有关。材料的流变曲线描述了形变过程中的变形历史和应变特性,有明显的阶段性,其中第Ⅱ阶段的变形历史和应变特性制约着裂纹的启裂过程,第Ⅲ阶段的变形历史和应变特性制约着裂纹的扩展过程。应变硬化率dσ/dε可以敏感地反映出材料内部的应力分布和微观断裂机制。     

Structural stability of single-layered LaNi4.25Al0.75 film and its electrochemical hydrogen-storage properties

AB5-based hydrogen storage thin fdms （LaNi4.25Al0.75）, deposited on Cu substrate by dc magnetron sputtering were investigated in this study. X-ray diffraction （XRD） revealed that the microstructure of the layer was in crystal form. SEM and AFM analyses proved that the film appeared to be rather rough with numerous randomly sized pores of approximately 15-40 in nm diameter. Structural stability of the film was examined by the combined analyses of DSC, XRD, and SEM, which indicated that this film maintained its structural stability below 500 K or so, and a network structure was observed on the film after being heated at 700 K for 30 min. Electrochemical hydrogen-storage properties of the films were investigated by simulated battery tests. It was found that single-layered LaNi4.25A10.75 film exhibited electrochemical hydrogen-storage properties similar to typical AB5 alloys in bulk, and the maximum discharge capacity of the film was about 220 mAh/g. After 20 charge/discharge cycles, small needle-shaped aluminium oxide was formed on some fractions of the film surface.     

不同变截面桩竖向承载特性的数值分析

基于现场试验,采用Abaqus有限元软件对等截面桩及变截面桩单桩竖向承载力测试实验过程进行数值模拟。结果表明:弹性变形阶段,竖向荷载-沉降曲线呈线性变化趋势,等截面桩比变截面桩桩身沉降较小;塑性屈服阶段,等截面桩与变截面桩单桩承载力几乎一致。变截面位置对桩侧摩阻力沿深度的分布有一定影响,最大侧摩阻力位置根据变截面的位置而变化。变截面桩的平均侧摩阻力较等截面桩的平均侧摩阻力大,变截面桩侧摩阻力效果较等截面桩更有优势。在相同承载力条件下,变截面桩所耗费的材料较等截面少,对于竖向承载特性,变截面桩与等截面桩表现基本一致,因此使用变截面桩能节约工程成本。     

内燃式热风炉燃烧器结构优化

针对内燃式热风炉在燃烧期烟气中CO含量超标问题开展研究工作,提出一种改进型的矩形燃烧器结构,在煤气通道中加入挡板来改变高炉煤气的流动方向。以某公司3号高炉热风炉为研究对象,建立了内燃式热风炉矩形燃烧器和燃烧室的三维模型。利用CFD模拟技术对矩形燃烧器的原始结构和改进后的结构进行燃烧模拟,在矩形燃烧器中加入的煤气挡板分别采用45°、60°、75° 3种倾斜角度放置,分析在不同倾斜角度下的温度场和CO浓度场。与原始结构的结果进行对比,结果表明,优化结构之后燃烧室出口截面的温度场中高温区范围有所扩大,两端眼角处的CO平均体积分数有一定程度减少。当煤气挡板的倾斜角度为60°时出口截面平均温度上升最大,平均温度从1 669 K上升到1 676 K,出口烟气中的CO平均体积分数下降最多,CO平均体积分数从0.007 028%下降到0.005 678%。     

型材弯曲模设计

分析了型材在弯曲后其口型失稳起皱的原因,介绍了在型材槽型中填充胶条后在模具的夹持中弯曲成形的工艺,从根本上解决了型材的失稳和起皱,保证了型材的口型尺寸。     

冷弯型钢特点及其生产发展

介绍了冷弯型钢的特点、生产工艺流程及其应用。在阐述分析冷弯型钢国内外生产现状的基础上，探索了它发展的方向。     

多元靶碰撞级联中的粒子能量

用一种推广的幂指数散射截面求解了溅射输运方程，获得更加一般的渐近解，这个渐近解不但以Urbassek等人几年前所给出的两个解为其特殊情况，而且还为作者提出的SC解提供了新的证据，此外通过计算并分析了Vicanek等人发表的新的输运理论，发现他们所提出的转换理论结果与Monte Carlo模拟结果一致是不正确的。     

钢铁构件表面上的无机富锌涂层

阐述了无机富锌涂层的研究、发展、特性和应用，指出无机富锌涂层具有杰出的耐环境侵蚀性能、耐化学性、耐溶剂性、耐辐射性、耐热性，在恶劣腐蚀环境下它是钢结构件的最佳底涂层。