H ∞ control for discrete‐time nonlinear Markov jump systems with multiplicative noise and sector constraint

This paper addresses the finite horizon H _∞ control problem for a class of discrete‐time nonlinear Markov jump systems with multiplicative noise and nonlinear feedback device. The system nonlinearity occurs in a random way specified by a Bernoulli process, whereas the actuator and sensor nonlinearities are restricted to a sector region. Both the state and the dynamic output feedback H _∞ controllers are devised in terms of difference LMIs. The proposed approach not only allows the resulting system to achieve a prescribed disturbance attenuation level, but also enables the output of actuator/sensor to meet the designated sector condition. Moreover, it is also shown that our approach is well‐adapted for dealing with the discrete‐time Markov jump systems with saturated actuator and sensor. Finally, a backward iterative algorithm is provided to solve the obtained difference LMIs and a numerical example is presented to verify the efficiency of the theoretical results. Copyright © 2013 John Wiley & Sons, Ltd.     

Robust adaptive control for a class of cascaded nonlinear systems with applications to space interception

This paper proposes a robust H _∞ ‐based adaptive backstepping control scheme for the output stabilization of a special class of cascaded nonlinear systems. This kind of systems possess the feature that the first sub‐equation is a linear perturbed system, whereas the rest ones perform a general semi‐strict feedback form. Different from the conventional backstepping design approach, the special cascaded structure ensures to introduce the H _∞ technique to the backstepping procedure such that both the robust performance and the robust stability can be simultaneously guaranteed. Within the Lyapunov framework, the proposed control scheme is proved to guarantee (i) the uniformly ultimate boundedness of the system signals with a bound that can be made arbitrarily small by suitably choosing control parameters; (ii) asymptotic output stabilization as long as the uncertain nonlinearities and external disturbances vanish; and (iii) ‐performance of the closed‐loop system. A space interception scenario is utilized to demonstrate the effectiveness of the proposed control scheme. Copyright © 2013 John Wiley & Sons, Ltd.     

一株枯草芽孢杆菌分离鉴定及其降解稠油特性

以稠油为唯一碳源,从被稠油污染过的土壤中筛选到一株高效石油烃降解茵,经生理生化鉴定和16S rDNA鉴定确认其为枯草芽孢杆茵.在摇瓶实验中,该菌最佳降解温度为35～45℃,最佳pH值为7.5～8.5,最佳盐质量浓度为8～16 g/L.在最佳降解条件下,当油质量浓度为0.1 g/L时,稠油降解率达34.3%.利用GC-nD分析知,该茵主要降解稠油中n-C9～n-C40的烷烃组分;利用GC-MS分析得知,该茵对蔡及烷基化萘去除彻底,对二苯并噻吩、芴和稠二萘等部分芳烃类化合物有降解作用,在稠油降解过程中菲及菲的衍生物有所增加.     

厌氧活性污泥PCR-DGGE图谱的优化

针对特种污泥PCR-DGGE图谱的优化一直是微生物分子生态学研究的重点.在反硝化抑制硫酸盐还原菌的研究中,研究厌氧硫酸盐还原污泥和反硝化污泥DNA的提取方法,应用通用引物958F/1401R扩增16S rDNA序列,探讨不同的退火温度对DGGE指纹图谱多样性影响,同时采用时间进程法对DGGE图谱进行优化.结果表明,采用PBS洗涤污泥和超声震荡有利于硫酸盐还原污泥DNA提取;采用958F/1401R为引物时,扩增片段大约500 bp,不同的退火温度对DGGE指纹图谱的多样性影响不显著;采用时间进程法可以很好地再现不同电泳阶段对样品的分离效果,最佳变性剂梯度为40%～60%,在130 V电压下,最佳电泳时间为5 h.     

Isolation and identification of a sulfate reducing bacteria and sequence analysis of its dissimilatory sulfite reductase gene

A sulfate reducing bacteria was isolated from mining sewage of Daqing Oilfield by Hungate anaerobic technology. Physiological-biochemical analysis showed that the strain could utilize polyacrylamide as sole carbon and nitrogen source. The sequence analysis of 16S rDNA illustrated that the similarity of F8 and Desulfovibrio desulfuricans (AF192153) was 99%, and the similarity sequence of dissimilatory sulfite reductase gene (DSR) cloned from the strain and Desulfovibrio desulfuricans (AF273034) was 98%. Their phylogenitic analysis was basically anastomosed, and thus temporarily named as Desulfovibrio desulfuricans F8. The DSR cloned from F8 strain was 2740 bp in length consisting of three ORF, DSRA, DSRB and DSRD as a single operon (DSRABD) regulated by the same operator. DSRA contained typical conservative box of sulfate—sulfite reducing enzyme (SiteⅠand SiteⅡ), which could bind siroheme and [Fe4S4]. DSRB retained a [Fe4S4] binding site, with an uncomplimentary structure for siroheme binding. There was no conservative box in DSRD. Sequence analysis of DSR will provide a theoretical basis for quantitative detection, metabolic pathway modification through gene engineering, and sulfate reducing bacteria (SRB) suppression.     

HPV16型免疫胶体金诊断试纸条的制备

建立一种快速、准确、简便检测高危型人乳头瘤病毒16型的胶体金诊断试纸条,以用于宫颈癌的早期筛查。用柠檬酸三钠还原法制备了酒红色的30 nm胶体金颗粒,胶体金颗粒标记的单抗的实际标记量为6μg/mL。选用了2种稳定剂BSA和PEG20000获得了稳定的金标抗体溶液。实验结果表明:第7种样品垫处理液和第7种结合垫处理液处理样品垫和结合垫组装的试纸条效果最好;试纸条检测HPV16E6蛋白为阳性,检测HPV16L1蛋白为阴性。     

一株微生物采油菌的16S rDNA鉴定和绿色荧光蛋白分子标记

筛选到一株高效生产多糖的YX菌,油田先导试验证明该菌可以用于微生物调剖提高原油采收率（Microbial Enhanced Oil Recovery,MEOR）.经16SrDNA分析鉴定,YX菌属于肠内杆菌属（Enterobacter.）,为跟踪监测其动态信息,构建表达绿色荧光蛋白（green fluorescent protein,GFP）的重组质粒pET-30a（＋）-EGFP,利用CaCl2法将其转化至YX菌,利用荧光显微镜可以观察到重组YX（GFP）工程菌的绿色荧光.结果表明YX菌可以被绿色荧光蛋白基因标记,用于微生物采油研究.     

基于IEEE 80211e EDCA的下行信道动态无竞争接入机制*

为了改善无线局域网络服务质量,IEEE工作组形成了增补标准IEEE 802.11e,但是其增强的接入机制仍然不能完全满足IPTV、视频点播等类似场景的应用。为此提出了针对这一类应用场景的一种新的简单易行的下行信道动态无竞争接入机制,该机制基于MAC层队列长度信息动态调整下行信道接入参数,能有效地保护下行流媒体数据的传输,通过仿真结果验证了该机制有效并且实用。     

IEEE802.16e中的卷积Turbo码编译码算法研究

为了分析不同设计参数对IEEE802.16e中卷积Turbo码(CTC)性能的影响以及对比IEEE802.16e支持的CTC和卷积码的编码性能,介绍了IEEE802.16e中的CTC的编码过程,给出了编码过程中的循环状态确定方法的推导过程,分析了CTC的迭代译码结构和译码算法,最后对CTC的性能进行了仿真.仿真结果表明:在相同码率和相同调制方式下CTC的编码增益要比卷积码大,采用CTC比采用卷积码有着明显的性能优势.因此在码率较低、信道状况较好或对系统实时性要求严格的情况下,可考虑使用卷积码,以降低信道编码的复杂度;而在码率较高、信道状况较差或对误码率要求严格时,可考虑选用CTC编码方案,以保证通信的可靠性.     

T C-1-HPV16 L1细胞模型与动物模型的建立与评价

为了解决人乳头瘤病毒16型L1蛋白(HPV16L1)为主要靶点的疫苗缺乏肿瘤模型细胞来验证疫苗的免疫效果的问题,构建了一个细胞模型并可以利用此细胞在实验动物体内形成肿瘤,用于以HPV16L1为主要靶点疫苗的验证实验。利用这个模型细胞或肿瘤模型可以在体内外检测疫苗的免疫学性质和保护功效。首先,利用聚合酶链式反应(polymerase chain reaction,PCR)的方法获得目标基因HPV16L1的基因序列并连接到载体质粒中,将质粒转染到细胞TC-1后在杀稻瘟菌素抗性压力筛选下获得稳定表达HPV16L1的细胞株。对TC-1和TC-1-HPV16L1两种细胞的生长增殖和成瘤特性进行比较发现,外源基因的加入对细胞特性没有影响。通过体外杀伤实验证实细胞TC-1-HPV16L1能够被HPV16L1靶点疫苗免疫过的小鼠脾淋巴细胞杀伤。实验动物被疫苗免疫后,进行攻瘤实验发现疫苗只对TC-1-HPV16L1组具有保护作用。综上,在本研究中成功地构建了可以检测HPV16L1疫苗的抑瘤效果的模型细胞TC-1-HPV16L1,为HPV疫苗的研究提供了一个模型细胞。