The determinants of heat-shock element-directed lacZ expression in Saccharomyces cerevisiae.

Heat-shock induction of heat-shock protein genes is due to a specific promoter element (the heat-shock element, HSE). This study used lacZ under HSE control (HSE-lacZ) to characterize HSE activity in Saccharomyces cerevisiae cells of different physiological states and differing genetic backgrounds. In batch fermentations HSE-lacZ induction by heat shock was maximal in exponential growth, and showed marked decline with the approach to stationary phase. Expression in the absence of heat shock was unaffected by growth phase, indicating that the growth-dependent expression of many yeast heat-shock genes uses promoter elements in addition to the HSE. Heat-induced expression was strongly influenced by the temperature at which cultures were grown. While basal, uninduced expression was constant during growth at different temperatures to 30 degrees C, induction by transfer to 39 degrees C was reduced by increases in growth temperature as low as 18-24 degrees C. Maximal HSE-lacZ induction (30- to 50-fold) was in cultures grown at low temperatures (18-24 degrees C), then heat shocked at 39 degrees C. Ethanol was a poor inducer. Mutations having little effect on HSE-lacZ expression included a respiratory petite; ubi4 (which inactivates the poly-ubiquitin gene); also ubc4 and ubc5 (which each inactivate one of the ubiquitin ligases involved in degradation of aberrant protein). pep4-3 increased both basal and induced beta-galactosidase about two-fold, probably because of slower turnover of this enzyme in pep4-3 strains.     

一种可用于可调谐环行Ti：Al_2O_3激光器的宽带单向器

本文介绍一种宽带单向器的原理及设计。选用ＴＧＧ作磁旋光介质，旋光石英晶片作补偿片，所设计的色散补偿型宽带单向器预计可在７００～１０００ｎｍ波长范围内实现环行激光腔中的单向行波运行；将其应用在可调谐环行Ｔｉ：Ａｌ２Ｏ３激光器中，获得了良好的实验结果。     

Analysis of a homologous series of non-reducing oligosaccharides from Arnica montana L. Roots

The oligosaccharide component compositions of a series of non-reducing oligosaccharides extracted from the roots of Arnica montana L. has been determined by gel permeation chromatography (g.p.c.). The range of oligosaccharides present was found to extend beyond the octasaccharide previously reported. with 16% to 19% of the oligosaccharides having a degree of polymerisation of between 11 and 19 and almost 3% having a degree of polymerisation greater than 20. The chromatographic behaviour of this series of oligosaccharides is compared with that observed for series of D-gluco-oligosaccharides.     

Emulsifying Properties of the Total Fraction and the Hydrophobic Fraction of Bovine Milk Proteose-peptones

Since proteose-peptone is a mixture of heterogeneous proteins and peptides, its functional properties have not yet been fully understood. The present study was undertaken to elucidate the emulsifying properties of proteose-peptones and a parallel evaluation was performed of the emulsifying activity of the total fraction prepared from bulk skimmed milk by precipitation with ammonium sulphate and of the most hydrophobic fraction purified by hydrophobic interaction fast protein liquid chromatography. A turbidimetric technique was used and the absorbance value at 500 nm was then directly used as the emulsifying activity index. The results obtained confirmed the good emulsifying activity of this protein fraction of the whey and demonstrated the considerable influence of the concentration on the capacity of the proteose-peptones to stabilize a model oil-in-water emulsion. Moreover, at all the concentrations examined, the purified component 3 showed a higher emulsifying activity than the total unpurified fraction.     

ANALYSIS OF EXPERIMENTAL AND NUMERICAL RESULTS OF A TURBULENT SWIRLING FLOW IN A TUBE

In many applications swirling flow is used to enhance heat and mass transfer. One of the problems of modelling a turbulent swirling flow is the choice of the turbulent closure model which is acceptable for engineering purposes. To evaluate which model performs best, numerical results are compared with experimental data. Local velocity measurements are carried out on a swirling flow in a circular tube. The measurement method is hot-wire anemometry combined with visualization techniques. The numerical analysis is carried out using the k-ε model and the Algebraic Stress Model. In the experiment a symmetrical swirl was observed, Comparing the experimental data with the numerical results shows that the Algebraic Stress Model represents the experimental data quite well, whereas the k-ε model fails.     

利用Visual C＋＋和Direct3D设计雷达显示器

利用Visual C＋＋和Direct3D对某雷达显示器的实现过程作了详细论述,包括数据采集、PPI型显示器、A型显示器、B型显示器、余晖显示、航迹显示等。通过试验证明,采用Direct3D绘图方式比采用GDI绘图方式效果好,更适用于数据处理量大、天线扫描速度快的雷达显示器。     

FTIR investigation of ethylene coordination and polymerization on reduced Cr/SiO2 catalyst

FTIR spectroscopy was applied to study the initial steps of ethylene polymerization on reduced chromia-silica (0.5 wt% Cr/SiO₂). To decrease the speed of the reaction small doses of gas were introduced to the catalyst in each run and C₂D₄ was used to confirm band assignments. At the initial steps of the reaction only ethylene molecules coordinated to probably Cr _A ²⁺ cations were observed. The concentration of such complexes was estimated to be about 50% of the total amount of Cr atoms in the sample. The FTIR spectrum of the polymer formed at the initial doses of C₂H₄ (when [C₂H₄] [Cr]) was found to be slightly different from that formed after excess ethylene was introduced onto the catalyst ([C₂H₄] > [Cr]).     

铝阳极氧化膜颜色的检验和观察法

介绍铝及铝合金阳极氧化膜颜色的概念和描写,对颜色的儿种测量方法进行了比较,重点分析观察法及其在各国标准中的异同。在此基础上,提出了GB8013中的对颜色进行观察的条件和理论根据。