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排序方式: 共有62条查询结果,搜索用时 718 毫秒
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Daniel G. Abernathy Gary Spedding Barry Starcher 《Journal of the Institute of Brewing》2009,115(2):122-127
In this study we present a ninhydrin based microwell assay that can be utilized in place of the traditional Kjeldahl method for the determination of the protein content of beer or wine. In addition, the assay is ideal for the determination of free amino acids in beer (FAN), a term understood and used by brewers, and yeast assimilable nitrogen (YAN) used by enologists. The assay only measures alpha amino acids and ammonia so other nitrogen sources are not detected, resulting in a 30% reduction in total protein of a variety of beers compared to the Kjeldahl method, which measures nitrogen from all sources. The results also showed that only 25% of the total “protein” in beer is actually derived from peptides larger than 3,500 Kd. Analysis of beer or wine with the microwell assay for total usable nitrogen was compared to the standard FAN and YAN methods and conditions were determined for maximal efficiency and precision. Superior results were obtained with low reaction volumes and a stable sodium acetate buffered ninhydrin reagent at pH 5.5. As an alternative, for use with cuvettes, a reduced volume FAN assay using the same pH 5.5 sodium acetate buffered ninhydrin reagent gave comparable results. The assay is economical, rapid, accurate and applicable to large numbers of samples. 相似文献
43.
Near-infrared (NIR) and mid-infrared (MIR) spectroscopy techniques were evaluated for the analysis of brewery worts. Good calibrations were obtained for maltose (SEPNIR 2.6 g/l, SEPMIR 2.0 g/l), glucose SEPNIR 0.6 g/l, SEPMIR 0.4 g/l) and the sum of fermentable sugars (SEPNIR 2.8 g/l, SEPMIR 2.6 g/l) with both methods. Satisfactory calibration was obtained for maltotriose (SEPNIR 1.4 g/l, SEPMIR 1.5 g/l), nitrogen (SEPNIR 122 mg/l, SEPMIR 92 mg/l) and free alpha-amino nitrogen (SEPNIR 29 mg/l, SEPMIR 23 mg/l). MIR spectroscopy gave slightly better results, but sample handling was more difficult than in the NIR region. The NIR measurement is simple and rapid and could be used for on-line process control during mashing. No sample preparation other than filtration is needed. 相似文献
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FAN5063是美国快捷公司生产的全兼容ACPI控制芯片,当用作ATX电源时,即可为PCI提供双3.3V电源,也可用SDRAM和RAM BUS供电,另外,FAN5063还集成有许多附加的保护功能。文中介绍了FAN5063芯片的功能,并给出了它的应用电路。 相似文献
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介绍了某控制转换装置测试仪的自动测试和故障诊断的设计思想,讨论了测试仪的硬软件设计中如何实现自动测试,以及利用FAN算法来计算故障定位测试向量的故障诊断思想和方法。该测试仪总体设计可靠性高,还有故障定位的功能,智能化程度高。可以在很大的程度上测试控制转换装置的功能和排除控制转换装置的故障。 相似文献
48.
CCFL被广泛用作大尺寸LCD的背光源。文中重点介绍了基于控制器FAN7548的四灯管CCFL电子变压器电路功能、特点及工作原理。 相似文献
49.
Near infrared (NIR) spectroscopy is a rapid technique which is used within plant breeding programs for the analysis of many grain traits. This study investigated the application of NIR analysis of wort samples to select malting quality cultivars in a barley breeding program. An automatic sampling and data capture system was developed which consisted of a Perten filter NIR instrument with a flow through cell module operating in transflectance mode interfaced with a computer, peristaltic pump and sample changer. Calibrations for hot water extract, free alpha-amino nitrogen and soluble protein were developed using multiple linear regression analysis based on four wavelength terms for each trait. The correlation coefficients for both calibration and prediction data sets were highly significant (P<0.01) and the standard error of prediction was similar to that obtained by standard methods. Cultivars with known malting quality were included in the experiments and their ranking by NIR was consistent with the standard methods. The reported calibrations have been used for over four years to screen early generation breeding lines for malting quality. 相似文献
50.
Okokon U. Etok Akpan 《Journal of the Institute of Brewing》2004,110(3):189-192
The diastatic power of the freshly kilned sorghum malt at 68.1°WK had a 29% drop after six months of storage. Freshly kilned sorghum malt displayed high wort turbidity (4.9 EBC) which dropped to 0.95 EBC and 1 EBC after 2 and 6 months of storage respectively. The colour of the malt worts faded slightly over the trial period from 7.6 EBC in freshly kilned malt to 6.8 EBC after six months. Extract remained fairly steady throughout the study period most likely due to the use of external amylolytic enzymes during mashing. The protein in extract/protein in grain fluctuated between 46.6% in the freshly kilned malt to 43.2% at the end of six months. The apparent extract after final attenuation (AEFA) indicates more fermentability beginning from two months after storage. Free α‐amino nitrogen (FAN) dropped from 238 mg/L to 194 mg/L after six months of storage. Mash filtration with a micro‐mash filter remained prolonged (86–93 min) throughout the six months of storage. 相似文献