Structures of the N-Terminal Domain of PqsA in Complex with Anthraniloyl- and 6-Fluoroanthraniloyl-AMP: Substrate Activation in Pseudomonas Quinolone Signal (PQS) Biosynthesis

Pseudomonas aeruginosa, a prevalent pathogen in nosocomial infections and a major burden in cystic fibrosis, uses three interconnected quorum-sensing systems to coordinate virulence processes. At variance with other Gram-negative bacteria, one of these systems relies on 2-alkyl-4(1H)-quinolones (Pseudomonas quinolone signal, PQS) and might hence be an attractive target for new anti-infective agents. Here we report crystal structures of the N-terminal domain of anthranilate-CoA ligase PqsA, the first enzyme of PQS biosynthesis, in complex with anthraniloyl-AMP and with 6-fluoroanthraniloyl-AMP (6FABA-AMP) at 1.4 and 1.7 Å resolution. We find that PqsA belongs to an unrecognized subfamily of anthranilate-CoA ligases that recognize the amino group of anthranilate through a water-mediated hydrogen bond. The complex with 6FABA-AMP explains why 6FABA, an inhibitor of PQS biosynthesis, is a good substrate of PqsA. Together, our data might pave a way to new pathoblockers in P. aeruginosa infections.     

A novel clipping technique for filtering FM signals embedded in intensive noise

A novel adaptive clipping technique for filtering a constant amplitude frequency modulated (FM) signal embedded in non-Gaussian noise is proposed. It is based on the analysis and processing of the estimate of probability density function of a FM signal realization. As a result, modifications of two robust estimators of FM signal amplitude are proposed. It is shown that these estimators can be used for Gaussian and non-Gaussian heavy-tail environments. The proposed clipping technique can exploit one or another obtained robust estimate of the signal amplitude for adaptive setting a threshold. Analysis of signal estimate accuracy for different noise environments is carried out. Comparative analysis of the obtained methods and known approaches based on scanning window nonlinear filtering and optimal robust L-DFT form is performed. It is demonstrated that the usage of clipping-based technique leads to the considerable improvement of the FM signal filtering efficiency in comparison to the aforementioned known approaches for different noise environments and a wide range of input SNR values.     

基于DAQ数据采集卡的虚拟仪器通用硬件平台设计

简介了虚拟仪器的基本概念和构成，讨论了虚拟仪器通用硬件平台的组成，详述了基于PCI总线的虚拟示波器的硬件设计思想，重点介绍了信号调理电路和数据采集电路的设计。     

冷轧高强HC250IF钢冲压分层原因分析及改进措施

针对冷轧高强HC250IF带钢冲压分层问题,通过化学成分分析、显微组织观察及能谱分析等手段,明确了铸坯中心P偏析是导致成品带钢冲压分层的根本原因。为此,采取钢水过热度20~30 ℃稳定浇注、提高连铸二冷水冷却强度、投用电磁搅拌、凝固末端精准定位、增加动态轻压下量等措施,有效改善了铸坯中心P偏析,杜绝了冷轧高强HC250IF带钢冲压分层问题。     

全光纤高速模拟信号偏振态测量系统的研究

全光纤高速偏振态测量,尤其是模拟信号的偏振态高速测量在光纤通信和光纤传感等领域中有着重要应用。在深入研究光电探测器放大电路的基础上,搭建了基于光纤偏振控制器(PC)、偏振分束器(PBS)以及模拟光电探测器的全光纤高速偏振态测量系统。用搭建的系统对0~50 MHz模拟信号的偏振态进行了测量,并与商用偏振分析仪进行了误差比较。实验表明,此偏振态测量系统可实现速率0~50 MHz模拟信号Stokes参量的准确测量,显示在邦加球上的测量误差半径在0.03以下,与商用偏振分析仪处于同一量级。系统重复性良好,且可测模拟信号的速率远超目前商用偏振分析仪,为许多待测光为模拟信号的光纤传感系统提供了有效的测量手段。     

一种双路图像融合实时处理系统的设计

提出了一种关于双路图像融合实时处理系统的设计,介绍了融合处理系统的总体架构和系统的功能。对系统的硬件设计和软件设计分别进行了说明,硬件设计对电源设计、采集模块设计和处理模块设计进行了详细阐述;软件部分细致分析了现场可编程门阵列(FPGA)的设计和数字信号处理器(DSP)的设计。最后对处理系统进行了测试,表明该设计满足实时处理的需求。同时,可对系统进行扩展,实现更多路图像的融合处理。     

一种基于北斗信号的星载双站SAR成像频率同步的方法

针对基于北斗信号的星载双站SAR成像频率同步误差,提出一种在高噪声情况下新的频率同步方法。以北斗卫星直达波信号为同步信号源建立同步通道,使用单周期积累的扩频信号优化时频捕获算法得到较精确伪码时延,在码同步后使用已知时延的多周期积累的时频捕获算法获到频率误差。连续10次计算机仿真在SNR=-50db的高噪声环境下频率误差均不超过1.5Hz。     

民航中波NDB导航信号监测方法探讨

本文主要研究了民航无线电导航信号监测方法,结合日常监测的工作经验,以乌鲁木齐国际机场NDB导航台为例多次测试分析,详细给出了针对NDB系统导航信号进行监测的技术方法。     

GNSS信号接收机的构件初识──GNSS导航信号的收发问题之十

GNSS信号接收机是GNSS导航卫星的用户设备,是实现GNSS卫星导航定位的终端仪器。它是一种能够接收、跟踪、变换和测量GNSS卫星导航定位信号的无线电接收设备,既具有常用无线电接收设备的共性,又具有捕获、跟踪和处理弱达3.5E-16W~2.5E-17W卫星微弱信号的特性。为此,GNSS信号接收机的构件必须满足这种捕获、跟踪和测量GNSS信号的需求。     

基于A*OMP算法及其改进算法的宽带雷达信号稀疏分解

传统稀疏分解算法正交匹配追踪(OMP)算法里采用内积最大值来寻找最优原子,该方法容易陷入局部最优,为了弥补这一缺点,采用了新的算法:A*OMP算法,该算法使用A*搜索(即最佳优先搜索技术)寻找最优原子,该搜索方式寻找的最优原子具有全局最优性。实验表明相比传统OMP算法而言,该算法有效地提高了信号的重构精度。