Post-Digital Transdisciplinarity

Why are buildings generally credited to a single individual or firm, without acknowledging the numerous other participants in their creation? Marjan Colletti , founder and director of the REX|LAB robotic experimentation laboratory at the University of Innsbruck, reflects on the nature and implications of transdisciplinarity. With reference to REX|LAB's contribution to the highly collaborative 2015 show of Iris van Herpen's Quaquaversal ready-to-wear collection, he further considers the increasingly understated approach to technological presence in the post-digital age.     

Sequencing of a 40·5 kb Fragment Located on the Left Arm of Chromosome VII from Saccharomyces cerevisiae

The nucleotide sequence of a 40·5 kb DNA fragment from the left arm of chromosome VII of Saccharomyces cerevisiae was determined and analysed. Twenty-eight open reading frames (ORFs) longer than 300 nucleotides were identified. Eight of them correspond to the following known yeast genes: EMP24, GCN1, SPO8, COX13, CDC55, RPS26, COX4 and LSR1, also called GTS1. Twelve ORFs are new, among them eight show homology with other genes while four have no homology with any sequence in the databases. Eight additional ORFs are internal to or partially overlapping with other ORFs. The nucleotide sequence reported here is deposited in the EMBL database under the Accession Numbers X91837 and X91489. © 1997 by John Wiley & Sons, Ltd.     

Sequencing of a 9·9 kb Segment on the Right Arm of Yeast Chromosome VII Reveals Four Open Reading Frames,including PFK1, the Gene Coding for Succinyl-CoA Synthetase (β-chain) and Two ORFs Sharing Homology with ORFs of the Yeast Chromosome VIII

A 9·9 kb DNA fragment from the right arm of chromosome VII of Saccharomyces cerevisiae has been sequenced and analysed. The sequence contains four open reading frames (ORFs) longer than 100 amino acids. One gene, PFK1, has already been cloned and sequenced and the other one is the probable yeast gene coding for the β-subunit of the succinyl-CoA synthetase. The two remaining ORFs share homology with the deduced amino acid sequence (and their physical arrangement is similar to that) of the YHR161c and YHR162w ORFs from chromosome VIII. The sequence is in the EMBL data library under Accession Numbers Z73024, Z73025, Z73026, Z73028 and Z73029.©1997 John Wiley & Sons, Ltd.     

Analysis of 21·7 kb DNA Sequence from the Left Arm of Chromosome VII Reveals 11 Open Reading Frames: Two Correspond to New Genes

The DNA sequence of a fragment of 21 731 bp (nucleotides 87408 to 109138) located on the left arm of chromosome VII from Saccharomyces cerevisiae S288C has been determined using a random cloning strategy followed by an oligonucleotide-directed sequencing. This fragment contains eight complete genes previously sequenced (CLG1, SKI8, VAM7, YPT32, MIG2, SIP2, SPT16 and CHC1), the 5′ part of POX1 and two other complete unidentified open reading frames of more than 100 amino acids. © 1997 John Wiley & Sons, Ltd.     

The deletion of six ORFs of unknown function from Saccharomyces cerevisiae chromosome VII reveals two essential genes: YGR195w and YGR198w

We have deleted six different ORFs of unknown function located on the right arm of Saccharomyces cerevisiae chromosome VII; namely, YGR187c/HGH1, YGR189c, YGR194c, YGR195w, YGR196c and YGR198w. No basic phenotypes could be attributed to the strains deleted in any of genes YGR187c/HGH1, YGR189c, YGR194c and YGR196c. These deletants did not show mating, sporulation or growth defects under any of the conditions tested. However, spores bearing deletions in either the YGR195w or YGR198w genes were unable to develop into macroscopical colonies. The YGR195w gene product shows significant homology with bacterial ribonuclease PH, an enzyme hitherto undescribed in yeasts, and its deletion causes a loss of viability after one to three rounds of cell division. Overexpression of this gene, using a tetracycline-regulatable promoter system, did not cause any effect on the cells. Contrary to what has been reported for prokaryotic homologs, this enzyme could play an essential role in yeast cell biology. The product encoded by the other essential ORF, YGR198w, shows no significant homology with any protein of known function in the databases. Spores bearing the deletion usually germinate and give rise to microcolonies of 50–100 non-viable cells. © 1998 John Wiley & Sons, Ltd.     

控规单元尺度城市更新中建筑碳排预计算研究 ——以武汉滨江商务区七期地块为例

我国已处于城镇化的后半程,加上“30 目标+60愿景”国际承诺的做出,标志着存量更 新和低碳规划将成为我国城市开发建设和转型 发展的基本语境。建筑碳排是城市中的重要碳 排放源,亦是影响城市更新的主要碳排因素和 计算因子,针对当前我国城市更新中建筑碳排计 算研究的缺失,以控规单元尺度为研究范围, 以建筑碳排预计算为研究对象,综合运用多种 定量研究方法与工具平台及图式化语言进行研 究分析。在能耗模拟框架下,对三种常用建筑碳排预计算方法进行比选,选取“根据规划用地类型与强度推算建筑能耗”的碳排计算方法。以武 汉滨江商务区七期地块为实证地块,根据城市更新规划设计方案,对城市更新后建筑碳排进行预 计算及结果分析;并对现状地块的建筑碳排进行计算,对比分析地块更新前后建筑能耗的减排 效应并进行原因探析,得出经过低碳城市更新后的建筑碳排降幅可达到约88%,主要原因在于高 能耗建筑类型的减少及用地功能与性质的改变。在实证研究的基础上,从三个方面提出控规单 元尺度城市更新的低碳规划策略与技术路径,最后对研究的科学价值和现实意义进行讨论,并 提出未来研究应继续努力与完善的方向。     

Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII

The nucleotide sequence of a 43 118 bp fragment from chromosome VII of Saccharomyces cerevisiae has been determined and analysed. The fragment originates from the right arm of chromosome VII. It starts approximately 11 kb centromere-proximal to the pet54 marker and ends in the middle of the PFK1 gene. The sequence contains a small nuclear RNA gene (SNR7) and 29 open reading frames (ORFs) larger than 100 amino acids. Six of these were completely internal to or partially overlapped other ORFs. Six previously described genes, YLM9/MRPL9, CRM1, DIE2, SMI1, PHO81 and YHB4, were mapped to this region in addition to pet54 and PFK1. Of the remaining 17 ORFs, four showed homology with other S. cerevisiae genes and four, including one of the partially overlapping ORFs, with genes from other organisms. Eight ORFs had no homology with any sequence in the databases. The actual sequences have been deposited in the EMBL database under Accession Number X87941.     

ERG1, encoding squalene epoxidase,is located on the right arm of chromosome VII of Saccharomyces cerevisiae

The ERG1 gene of Saccharomyces cerevisiae encodes squalene epoxidase, a key enzyme in the ergosterol pathway. ERG1 is an essential gene. Disruption of the gene with URA3 results in a lethal phenotype when cells are grown under aerobic conditions, even in the presence of ergosterol. However, cells are viable in the presence of ergosterol under anaerobic growth conditions during which ergosterol is taken up by cells. Physical and genetic mapping data reveal that ERG1 is located on the right arm of chromosome VII proximal to QCR9 at a distance of 14·6 cM from ADE3.     

影响乙丙共聚物粘度指数改进剂剪切稳定指数因素的分析

粘度指数改进剂作为调制多级润滑油的主要添加剂之一,它自身质量高低对多级油的品质起了至关重要的作用。文章针对影响粘度指数改进剂重要指标剪切稳定指数的主要因素,从原材料的使用、生产工艺的确定、设备运行操作等几个方面进行了查找和分析,为生产高品质粘度指数改进剂积累了一定的生产经验并优化了生产工艺条件。