Cell处理器上软件缓存的设计与实现

在 Cell异构多核处理器上,并行程序对不规则共享数据的访问延迟较大,共享数据的一致性维护困难。为解决上述问题,提出一种基于扩充Location Consistency存储模型一致性协议的软件缓存。测试结果表明,该软件缓存能够缩短近40%的共享数据访问时间,有效提高并行程序的执行效率。     

Internet use, videogame playing and cell phone use as predictors of children’s body mass index (BMI), body weight, academic performance, and social and overall self-esteem

In this research we examined the prediction of children’s body mass index (BMI), body weight, academic performance, social self-esteem and overall self-esteem from their IT (information technology) use, specifically, their Internet use, cell phone use and videogame playing, after controlling for the effects of gender, race, age and household income on these measures. Participants were 482 children whose average age was 12 years old. One-third was African American and two-thirds were Caucasian American. Results indicated that IT use did not predict BMI or body weight, contrary to one previous survey and the widespread belief that screen time is responsible for the obesity epidemic among our nation’s children. Instead, BMI and body weight were higher for African Americans, older children and children from lower income households. The sole and strong positive predictor of visual-spatial skills was videogame playing, which also predicted lower grade point averages (GPAs). Gender and Internet use predicted standardized test scores in reading skills. Females and children who used the Internet more had better reading skills than did males and children who used the Internet less, respectively. Implications of these findings for future research on the benefits and liabilities of IT use are discussed.     

An efficient tabu search algorithm to the cell formation problem with alternative routings and machine reliability considerations

Cell formation is the first step in the design of cellular manufacturing systems. In this study, an efficient tabu search algorithm based on a similarity coefficient is proposed to solve the cell formation problem with alternative process routings and machine reliability considerations. In the proposed algorithm, good initial solutions are first generated and later on improved by a tabu search algorithm combining the mutation operator and an effective neighborhood solution searching mechanism. Computational experiences from test problems show that the proposed approach is extremely effective and efficient. When compared with the mathematical programming approach which took three hours to solve problems, the proposed algorithm is able to produce optimal solutions in less than 2 s.     

DRIE based technology for 3D silicon barcodes fabrication

Microworld barcoding has become a promising tool for cell biology. Individual and subpopulation cell tracking is of great interest to evaluate cell behaviour. Nowadays, many micrometer and even nanometer size silicon structures can be fabricated using microelectronics techniques. In this work we report for first time the development of 3D barcodes based on silicon substrate. The proposed silicon micromachining technology based on deep reactive ion etching (DRIE) allows to obtain micrometer-sized cylindrical structures with vertical etch profile that defines a bit = 1 and non-vertical etch profile that defines a bit = 0. Although this technology will allow more than 15 bits representation, only 4-8 bits are necessary for cell labelling. The results of this work show that DRIE has become a versatile technique to produce high aspect 3D biocompatible silicon-based barcodes structures for cell studies.     

Development of high throughput microfluidic cell culture chip for perfusion 3-dimensional cell culture-based chemosensitivity assay

This study reports a microfluidic cell culture chip encompassing 36 microbioreactors for high throughput perfusion 3-dimensional (3D) cell culture-based chemosensitivity assays. Its advantages include the capability for multiplexed medium delivery, and the function for both efficient and high throughput micro-scale 3D culture construct preparation and loading. The results showed that the proposed medium pumping mechanism was able to provide a uniform pumping rates ranging from 1.2 to 3.9 μl h⁻¹. In addition, the simple cell/hydrogel loading scheme has been proven to be able to carry out 3D cell culture construct preparation and loading precisely and efficiently. Furthermore, a chemosensitivity assay was successfully demonstrated using the proposed cell culture chip. The results obtained were also compared with the same evaluation based on a conventional 2D monolayer cell culture. It can be concluded that the choice of cell culture format can result in different chemosensitivity evaluation results. Overall, because of the nature of miniaturized perfusion 3D cell culture, the cell culture chip not only can provide stable, well-defined and more biologically relevant culture environments, but it also features low consumption of research resources. All these traits are found particularly useful for high-precision and high-throughput 3D cell culture-based assays.     

Surface plasmon resonance (SPR) sensors for the rapid, sensitive detection of the cellular response to osmotic stress

Cells regulate their volume in response to changes in osmolarity of both, their extracellular and intracellular environments. As stability of the cell volume is a compelling exigency for cellular integrity, techniques for a sensitive, time-resolved volume measurement of adherently grown mammalian cells attract considerable interest, especially in the field of cell physiology and biology. In this study we apply a surface plasmon resonance (SPR) based biosensor for the comparative analysis of the volume responses of two renal epithelial cell types to non-isotonic challenges. The on-line, label-free and non-invasive biosensor format shows distinct similarities and differences in the reaction kinetics of the two cell types. Furthermore regulatory volume responses to the osmotic stimuli as well as their inhibition by Gd³⁺ ions can be observed with a high time-resolution. Limit-of-detection measurements indicate the high sensitivity of the sensor capable of detecting cellular volume responses of adherently grown mammalian cells to osmotic stimuli well below a bioanalytical relevant value of 5 mOsm/kg.     

Automatic joint classification and segmentation of whole cell 3D images

We present a machine learning tool for automatic texton-based joint classification and segmentation of mitochondria in MNT-1 cells imaged using ion-abrasion scanning electron microscopy (IA-SEM). For diagnosing signatures that may be unique to cellular states such as cancer, automatic tools with minimal user intervention need to be developed for analysis and mining of high-throughput data from these large volume data sets (typically ). Challenges for such a tool in 3D electron microscopy arise due to low contrast and signal-to-noise ratios (SNR) inherent to biological imaging. Our approach is based on block-wise classification of images into a trained list of regions. Given manually labeled images, our goal is to learn models that can localize novel instances of the regions in test datasets. Since datasets obtained using electron microscopes are intrinsically noisy, we improve the SNR of the data for automatic segmentation by implementing a 2D texture-preserving filter on each slice of the 3D dataset. We investigate texton-based region features in this work. Classification is performed by k-nearest neighbor (k-NN) classifier, support vector machines (SVMs), adaptive boosting (AdaBoost) and histogram matching using a NN classifier. In addition, we study the computational complexity vs. segmentation accuracy tradeoff of these classifiers. Segmentation results demonstrate that our approach using minimal training data performs close to semi-automatic methods using the variational level-set method and manual segmentation carried out by an experienced user. Using our method, which we show to have minimal user intervention and high classification accuracy, we investigate quantitative parameters such as volume of the cytoplasm occupied by mitochondria, differences between the surface area of inner and outer membranes and mean mitochondrial width which are quantities potentially relevant to distinguishing cancer cells from normal cells. To test the accuracy of our approach, these quantities are compared against manually computed counterparts. We also demonstrate extension of these methods to segment 3D images obtained using electron tomography.     

Recognition and analysis of cell nuclear phases for high-content screening based on morphological features

Automated analysis of molecular images has increasingly become an important research in computational life science. In this paper some new and efficient algorithms for recognizing and analyzing cell phases of high-content screening are presented. The conceptual frameworks are based on the morphological features of cell nuclei. The useful preprocessing includes: smooth following and linearization; extraction of morphological structural points; shape recognition based morphological structure; issue of touching cells for cell separation and reconstruction. Furthermore, the novel detecting and analyzing strategies of feed-forward and feed-back of cell phases are proposed based on gray feature, cell shape, geometrical features and difference information of corresponding neighbor frames. Experiment results tested the efficiency of the new method.     

呼叫准入约束下Macro-Femto小区选择GSPN模型研究

随着数据业务快速增长,仅靠宏蜂窝网络已无法满足流量需求;飞蜂窝网络可以有效分流宏蜂窝流量, 近年来 所构成的Macro-Femto架构得到了较广泛的部署。Macro-Femto网络部署的关键问题之一是小区选择,由于飞蜂窝网络频谱资源有限,进行小区选择时仍需考虑呼叫准入控制(Call Admission Control,CAC)。图形化的广义随机Petri网(Generalized Stochastic Petri Nets,GSPN)具有并行性、不确定性和异步特性以及强大的分析复杂系统的功能。针对Macro-Femto网络的呼叫准入约束机制,通过分析呼叫对信道的占用情形,在改进呼叫重审机制的基础上,建立了呼叫准入控制策略GSPN模型。利用建立的GSPN模型,分析了不同呼叫准入控制策略对新呼叫和切换呼叫阻塞率的影响,给出了部分重审机制的呼叫准入控制策略。在分析飞蜂窝的高速服务特性和宏蜂窝的高负载能力的基础上,结合部分重审呼叫准入策略和GSPN方法,提出了基于负载的飞蜂窝优先选择方案(Load-Based Femto-Priority Selection Scheme,LFP)。仿真结果表明,较典型的飞蜂窝优先选择方案,所提出的LFP方案对新呼叫的阻塞率和切换呼叫的掉线率分别降低了2.7%和4.6%。     

Mobile phone use among college students is a sedentary leisure behavior which may interfere with exercise

Research suggests that cell phone use is related to sedentary behavior, that cell phone use during exercise reduces intensity, and that high frequency cell phone users are less fit than other users. Thus, cell phone use appears connected to health and fitness behaviors and should be better understood within this context. The present study investigated the sedentary nature of cell phone use, and examined the likelihood of cellphone use interfering with exercise behavior.DesignA validated survey was administered to a random sample of students from a public US university (N = 226).ResultsMean self-reported cell phone use was 380 min day⁻¹, 87% reported cell phone use primarily occurs while sitting, and 70% of use was for leisure. Cell phone use was positively related to sedentary behavior (β = 0.30, p < 0.001). It was not related to physical activity. However, the likelihood of cell phone use during moderate (p = 0.006) and mild (p < 0.001) intensity exercise increased as cell phone use increased.ConclusionLike other screens (e.g., TVs), cell phone use appears to be a sedentary leisure behavior. Furthermore, high frequency use increases the likelihood that it will occur during exercise, likely lowering exercise intensity.