Molecular interaction networks in the analyses of sequence variation and proteomics data

Protein–protein interaction networks are typically generated in standard cell lines or model organisms as it is prohibitively difficult to record large interaction datasets from specific tissues or disease models at a reasonable pace. Although the interaction data are of high confidence, they thus do not reflect in vivo relationships as such. A wealth of physiologically relevant protein information, obtained under different conditions and from different systems, is available including information on genetic variation, protein levels, and PTMs. However, these data are difficult to assess comprehensively because the relationships between the entities remain elusive from the measurements. Here, we exemplarily highlight recent studies that gained deeper insight from genetic variation, protein, and PTM measurements using interaction information pointing toward the importance and potential of interaction networks for the interpretation of sequencing and proteomics data.     

基于MC9S12NE64的以太网测控系统的设计

嵌入式测控系统是当前工业自动化测控应用领域研究的热点之一。本文利用Freescale公司的MC9S12NE64MCU,运用其内部10位A/D模块,进行数据采集,并通过其集成的以太网网络模块实现了数据上传和远程控制。     

学校学生管理系统的设计与实现

随着教育的发展,学校规模不断扩大,专业不断增设,学生数量逐渐增加,有关学生的各种信息也在增多。面对大量的信息,学校特别需要专门管理学生信息的系统。本文设计开发了学校学生管理系统,该系统使用VB作为开发工具,利用面向对象的结构化程序设计方法,采用了MS SQL Server关系数据库管理系统,基本实现了学校学生学籍档案及成绩等管理,提高了学生管理的效率,完成了快速查询所需信息及及时更新的功能。系统编制过程中充分考虑了用户的需求,用户界面十分简洁、实用,层次结构合理。     

USB控制芯片PDIUSBD12典型应用研究

该文首先介绍对PDIUSBD12的结构、原理和特性进行了简单介绍,给出了PDIUSBD12与AT89S51连接的电路原理图。以ATMEL单片机和PHILIPS公司的PDIUSBD12构成USB设备控制端,对PDIUSBD12芯片及应用进行研究。     

基于LabVIEW的食管动力检测系统设计

设计了一种基于虚拟仪器技术的食管动力检测系统,可以实现食管动力信号的实时采集、显示以及数据的自动记录与分析处理等功能,用于消化道动力障碍性疾病的辅助诊断。本文介绍了系统的硬件组成、下位机设计和LabVIEW环境下的上位机软件设计。     

时间序列季节调整方法在中国的发展:PBC版X-12-ARIMA

中国人民银行(PBc)版X-12-ARIMA软件是基于中国特点而定制的时间序列季节调整软件.通过总结时间序列季节调整方法的特点以及相应软件在国外的发展,针对我国应用的特点,尤其是春节因素的考虑,在解剖X-12-ARIMA方法原理的基础上,在春节因素计算方法、软件应用界面以及用户使用帮助等3个主要方面加以改进,具有数据导入、调整设置文件、运行方式以及结果输出4方面的特色.     

Aberrant protein expression in plasma and kidney tissue during experimental obstructive nephropathy

Kidney failure is a major health problem worldwide. Patients with end-stage renal disease require intensive medical support by dialysis or kidney transplantation. Current methods for diagnosis of kidney disease are either invasive or insensitive, and renal function may decline by as much as 50% before it can be detected using current techniques. The goal of this study was, therefore, to identify biomarkers of kidney disease (associated with renal fibrosis) that can be used for the development of a non-invasive clinical test for early disease detection. We utilized two protein-profiling technologies (SELDI-TOF MS and 2-D) to screen the plasma and kidney proteome for aberrantly expressed proteins in an experimental mouse model of unilateral uretric obstruction, which mimics the pathology of human renal disease. Several differentially regulated proteins were detected at the plasma level of day-3-obstructed animals, which included serum amyloid A1, fibrinogen α, haptoglobin precursor protein, haptoglobin and major urinary proteins 11 and 8. Differentially expressed proteins detected at the tissue level included ras-like activator protein 2, haptoglobin precursor protein, malate dehydrogenase, α enolase and murine urinary protein (all p<0.05 versus controls). Immunohistochemistry was used to confirm the up-regulation of fibrinogen. Interestingly, these proteins are largely separated into four major classes: (i) acute-phase reactants (ii) cell-signaling molecules (iii) molecules involved in cell growth and metabolism and (iv) urinary proteins. These results provide new insights into the pathology of obstructive nephropathy and may facilitate the development of specific assay(s) to detect and monitor renal fibrosis.     

Reference Range Estimation: Accounting for Measurement System Errors

Reference range is a statistic that is used in health related fields to represent the range of the most likely values for a variable of interest. Based on this range, individuals are classified as being healthy or unhealthy. In biostatistics, the reference range is calculated as the (1 ? α)% prediction interval, where this prediction interval is based on the estimated population variance from the data. Such estimation of population variance is not precise, because obtained test results do usually have errors associated with them. These errors are due to the imprecise test procedure or gauge used. In this paper, the total variability in the data is decomposed into two categories. The first is the patient‐to‐patient variability and the other is the variability due to the measurement system used. Estimation of the two kinds is performed through a gauge repeatability and reproducibility study, then the reference range is calculated, taking into account only the patient‐to‐patient variability. The revised reference range procedure is illustrated through a case study of vitamin B12 test results. A closed form formula is given to calculate the probability of a given test result being within the revised reference range. Copyright © 2015 John Wiley & Sons, Ltd.     

Effects of T5 heat treatment on extrusion welds in AZ80 hollow profile

AZ80 hollow profile was produced by porthole-die extrusion. The mechanical behavior and microstructure characteristic of extrusion welds in AZ80 hollow profile during different T5 heat treatments were investigated. Five kinds of morphologies of β-Mg₁₇Al₁₂ were observed in turn in weld regions under various temperatures. Extrusion weld is not weakest region in AZ80 hollow profile in terms of hardness, the peak hardness of weld regions is roughly equal to the weld-free regions at different aging temperatures, which is attributed to similar volume fraction, morphology, and size of β-Mg₁₇Al₁₂ precipitate in weld regions and weld-free regions. The precipitate-free zones (PFZs) usually form near the welds interface in aging process, and the hardness of PFZs is usually lower than those of weld regions and weld-free regions. Aging at 200°C for 24?h is suitable heat treatment system for AZ80 hollow profile, which can achieve excellent mechanical property and simultaneously reduce the width of PFZs.     

Bending impact on the performance of a flexible Li4Ti5O12-based all-solid-state thin-film battery

The growing demand of flexible electronic devices is increasing the requirements of their power sources. The effect of bending in thin-film batteries is still not well understood. Here, we successfully developed a high active area flexible all-solid-state battery as a model system that consists of thin-film layers of Li₄Ti₅O₁₂, LiPON, and Lithium deposited on a novel flexible ceramic substrate. A systematic study on the bending state and performance of the battery is presented. The battery withstands bending radii of at least 14 mm achieving 70% of the theoretical capacity. Here, we reveal that convex bending has a positive effect on battery capacity showing an average increase of 5.5%, whereas concave bending decreases the capacity by 4% in contrast with recent studies. We show that the change in capacity upon bending may well be associated to the Li-ion diffusion kinetic change through the electrode when different external forces are applied. Finally, an encapsulation scheme is presented allowing sufficient bending of the device and operation for at least 500 cycles in air. The results are meant to improve the understanding of the phenomena present in thin-film batteries while undergoing bending rather than showing improvements in battery performance and lifetime.