Excessive biomass formation in two-phase flow trickle-bed bioreactors induces biological clogging and leads to the progressive obstruction of the bed that is accompanied with a build-up in pressure drop and flow channeling. One of the important aspects during biological clogging in trickle-bed bioreactors is the aggregation of cells and the detachment of cells and aggregates from pore bodies within the porous bed. Current theoretical models describing the transient behaviour of biomass accumulation and the biological clogging in trickle-bed bioreactors for wastewater treatment neglect the cell aggregation process and the aggregates detachment. An attempt has been made with this contribution in which the authors strived to develop an Euler-Euler two-fluid dynamic model based on the volume-average mass, momentum equations, species balance equations, biomass dynamics equation, filtration equations for the cells and the aggregates and discrete population balance equations for the cells agglomeration to describe two-phase flow and space-time evolution of biological clogging in trickle-bed bioreactors for wastewater treatment. Phenol biodegradation by Pseudomonas putida as the predominant species immobilized on activated carbon was chosen as a case study to illustrate the consequences of formation of excessive amounts of biomass. Cells aggregation was described by the rate at which a certain size aggregate is being formed by smaller aggregates minus the rate at which the aggregate combines to form a larger aggregate. The detachment of the cells or aggregates from the collector surface was supposed to be induced by the colloidal forces in the case of Brownian cells/aggregates or by the hydrodynamic forces in the case of non-Brownian aggregates. 相似文献
The aim of this study was a better understanding of the regulation mechanisms of in vitro osteoblast activity on biomaterials. Rat osteoblast behaviour on different surfaces was studied. Surfaces with different roughness (and a similar surface chemistry) or with different surface chemistry (and a similar roughness) were compared. Cellular morphology was observed by scanning electron microscopy and cell adhesion was quantified using an image analysis system. Osteoblast proliferation was quantified by a MTT test and total protein content and alkaline phosphatase (ALP) activity were evaluated by spectrophotometry. Data were compared by statistical analysis.
Results showed that NiTi surface roughness did not influence osteoblasts morphology, adhesion, total protein content and ALP activity whereas it modulated cell proliferation. Roughness was shown to stimulate cell proliferation. For smooth surfaces exhibiting two different chemical compositions, adhesion rate was found to be higher on Thermanox® than on NiTi whereas proliferation was shown to be smaller. ALP activity was also modulated by surface chemistry. Thus, cell adhesion and ALP activity were found to be more governed by surface chemistry than by roughness whereas cell proliferation was shown to be modulated by roughness (this effect increasing during cell culture) and by chemistry (this effect remaining stable in time) together. Total protein content and cell morphology were found to be independent of both parameters (roughness and chemistry). Effects of surface chemistry were discussed in terms of wettability and electron acceptor/donor properties of the surfaces of interest. Immunofluorescence images of adhesion proteins could not demonstrate differences between the three surfaces. 相似文献
The inorganic membrane reactor is a combined unit operation of chemical reactions and membrane separations. By having a membrane reactor, the downstream separation load can be reduced. Also, the yields can be increased and conversion can be improved for equilibrium limited reactions. However, many of the industrial chemical reactions take place at high temperature that the conventional polymeric membranes cannot withstand. A great deal of research has been done recently to develop ion-conducting ceramic membranes. Many of these have been successfully employed to form membrane reactors for many industrially relevant chemical reactions, such as hydrogenation, dehydrogenation, oxidation, coupled reactions, and decomposition reactions. An overview is given for the area of inorganic membrane preparations and membrane reactors. Many examples of petrochemical interests are presented, including hydrocarbon conversions and fuel cell applications. 相似文献
Perovskite based on Sn have attracted extensive attention to address the toxicity challenge associated with Pb-based perovskite solar cells. However, Sn-based perovskite solar cells(SPSCs)are notable for their poor stability and loss of efficiency due to rapid oxidation of Sn2+ to Sn4+ in air. To slow down the rapid oxidation, a number of antioxidants are suggested. Nevertheless, the antioxidant normally gets oxidized to non-antioxidizing by-products in a single-stage redox reaction and loses the function of oxidation prevention. Herein, vanillin is introduced, a natural antioxidant with a double-staged redox reaction to inhibit the oxidation of Sn2+ or reduce Sn4+ back to Sn2+, which improves the efficiency and prolongs the open-air stability of SPSCs. With 7.5% vanillin doping, an outstanding efficiency of 13.18% is achieved. Moreover, exposure of the solar cell to 160 W microwave irradiation for 3 minutes resulted in significant efficiency recovery from 88% to 96.5% at 812 hours and from 35.7% to 65.4% after 2200 hours of aging. This work reveals the potential of natural antioxidation and short microwave irradiation as suitable approaches to elevate the efficiency and lifetime of SPSCs. 相似文献
As the interest in hydrogen and PEM fuel cells is growing, it is crucial to define the best technology for fuel storage, especially in the transportation field. Metal hydrides show different benefits, including the possibility of thermally coupling the hydrogen storage and utilization systems: fuel cells require heat subtraction for ensuring proper operation, while metal hydrides require heat to activate the hydrogen release reactions. This work describes the integration of PEMFCs and metal hydrides on board a zero-emissions ship, with a special focus on their thermal coupling; a model-based approach is developed to ensure the system's feasibility at different load demands for the vessel, including transient conditions. The study is based on the real application of an innovative zero-emissions ship (ZEUS) financed by Fincantieri-Isotta Fraschini S.p.A, where the total power installation is set at 144 kW by PEMFC and 50 kg of hydrogen are stored by metal hydrides. 相似文献
Internalization and dynamic subcellular distribution of thiol-capped CdTe quantum dots (QDs) in living cells were studied
by means of laser scanning confocal microscopy. These unfunctionalized QDs were well internalized into human hepatocellular
carcinoma and rat basophilic leukemia cells in vitro. Co-localizations of QDs with lysosomes and Golgi complexes were observed,
indicating that in addition to the well-known endosome-lysosome endocytosis pathway, the Golgi complex is also a main destination
of the endocytosed QDs. The movement of the endocytosed QDs toward the Golgi complex in the perinuclear region of the cell
was demonstrated.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
This paper reports the production of high quality copper indium diselenide thin films using pulsed DC magnetron sputtering from a powder target. As-grown thin films consisted of pin-hole free, densely packed grains. X-ray diffraction showed that films were highly orientated in the (112) and/or (204)/(220) direction with no secondary phases present. The most surprising and exciting outcome of this study was that the as-grown films were of near stoichiometric composition, almost independent of the composition of the starting material. No additional steps or substrate heating were necessary to incorporate selenium and create single phase CuInSe2. Electrical properties obtained by hot point probe and four point probe gave values of low resistivity and showed that the films were all p-type. The physical and structural properties of these films were analyzed using X-ray diffraction, scanning electron microscopy and atomic force microscopy. Resistivity measurements were carried out using the four point probe and hot probe methods. The single step deposition process can cut down the cost of the complex multi step processes involved in the traditional vacuum based deposition techniques. 相似文献