跨部门的PDT激励机制研究

针对企业跨部门项目团队内缺乏有效的团队成员激励机制的问题,在对项目成员的能力、风险规避度和团队化程度等因素分析的基础上,提出新的生产函数.基于委托代理理论,建立委托代理激励机制模型并求解,然后对影响PDT项目团队的各因素进行了灵敏度分析,以期为先进生产型企业中跨部门PDT激励机制的建立提供行之有效的指导.经过分析发现,...     

Thermally Managed,Injectable Optoelectronic Probe with Heat Dissipation Guide for Photodynamic Therapy

The development of fabrication technologies and appearance of new materials has resulted in dramatic increase in the performance of electronic devices, while the overall size has decreased. Recent electronic devices made of micro/nano-size components show high efficiency and outstanding performance with compact size, but these devices have revealed several fatal problems. In particular, the isolated heat that is generated by numerous components concentrated in a limited small area at high density, such as bio-integrated devices, is an issue that needs to be urgently addressed, because it is closely related to the performance and lifetime of electronic devices. To solve these problems, the microscale light emitting diode (µLED)-based neural probe is introduced on an injectable heat dissipation guide. The heat dissipation guide is made of boron nitride (BN) nanomaterials with high thermal conductivity. The heat management noticeably improves the optical output performance of the µLEDs, in which BN effectively dissipates heat, and allows enhanced lighting from the LEDs to be transmitted through brain tissue without thermal damage. Moreover, it shows remarkable improvement in the therapeutic effect of photodynamic therapy of mouse cancer cells.     

基于ALA的PDT体外灭活HL60实验研究

利用自行设计的光动力疗法(PDT)反应室对HL60细胞进行了氨乙酰丙酸(ALA)PDT实验研究，获得灭活HL60细胞的最佳光学参量因子：ALA培养的终浓度为1．0mM／ml；ALA的培养时间为4h；辐射光波长为412nm；辐照光剂量为18J／cm^2；辐照光功率为5mW／cm^2；培养细胞的胎牛血清(FCS)浓度为8％～10％。在这些光学参数下对HL60的灭活能达89．9％，而单核淋巴细胞(PBMC)的损伤仅为15％，基本上能达到选择性地灭活肿瘤细胞HL60。     

Fluorinated Polyethylenimine to Enable Transmucosal Delivery of Photosensitizer‐Conjugated Catalase for Photodynamic Therapy of Orthotopic Bladder Tumors Postintravesical Instillation

Photodynamic therapy (PDT) by insertion of an optical fiber into the bladder cavity has been applied in the clinic for noninvasive treatment of bladder tumors. To avoid systemic phototoxicity, bladder intravesical instillation of a photosensitizer may be an ideal approach for PDT treatment of bladder cancer, in comparison to conventional intravenous injection. However, the instillation‐based PDT for bladder cancer treatment remains to be less effective due to the poor urothelial uptake of photosensitizer, as well as the tumor hypoxia‐associated PDT resistance. Herein, it is uncovered that fluorinated polyethylenimine (F‐PEI) achieved by mixing with Chorin‐e6‐conjugated catalase (CAT‐Ce6) is able to form self‐assembled CAT‐Ce6/F‐PEI nanoparticles, which show greatly improved cross‐membrane, transmucosal, and intratumoral penetration capacities compared with CAT‐Ce6 alone or nonfluorinated CAT‐Ce6/PEI nanoparticles. Owing to the decomposition of tumor endogenous H₂O₂ by CAT‐Ce6/F‐PEI nanoparticles penetrating into bladder tumors, the tumor hypoxia would be effectively relieved to further favor PDT. Therefore, bladder intravesical instillation with CAT‐Ce6/F‐PEI nanoparticles could offer remarkably improved photodynamic therapeutic effect to destruct orthotopic bladder tumors with reduced systemic toxicity compared to hematoporphyrin, the first‐line photosensitizer used for bladder cancer PDT in clinic. This work presents a unique photosensitizer nanomedicine formulation, promising for clinical translation in instillation‐based PDT to treat bladder tumors.     

HPPH光动力治疗对鼠G422脑胶质瘤突变型p53蛋白和p16蛋白表达的影响

目的:探讨HPPH光动力治疗对鼠G422脑胶质瘤突变型p53蛋白和p16蛋白表达的影响并和HpD做比较。方法:建立鼠G422脑胶质瘤皮下移植瘤模型,设立空白对照组、单注射HPPH组(0.45mg/kg)、单665nm激光照射组、HPPH-PDT组(0.15mg/kg组、0.3mg/kg组、0.45mg/kg组)、HpD-PDT组(5mg/kg)。单注射HPPH组、HPPH-PDT组和HpD-PDT组自尾静脉推注入光敏剂,24h后以波长665nm的半导体激光照射HPPH-PDT组和单激光组肿瘤,功率密度200mW/cm~2,每光斑照射17min,能量密度为204J/cm~2;以波长630nm的半导体激光照射HpD-PDT组肿瘤,功率密度200mW/cm~2,每光斑照射20min,能量密度为240J/cm~2。于PDT后9d处死鼠行酶联免疫吸附法(ELISA)的双抗体夹心法检测HPPH-PDT和HpD-PDT后突变型p53蛋白(mutant type p53 protein,mtp53)和p16蛋白表达变化。结果:空白、单注药和单照光三组对照组之间mtp53蛋白和p16蛋白表达值两两比较,差异无统计学意义(P>0.05)。HPPH-PDT各剂量组与空白对照组比较,mtp53蛋白表达值明显降低,p16蛋白表达值明显升高,差异有统计学意义(P<0.01)。0.3mg/kg组和0.45mg/kg组与0.15mg/kg组相比,mtp53蛋白表达值降低,p16蛋白表达值升高,差异有统计学意义(P<0.05),0.45mg/kg组mtp53蛋白表达值稍低于0.3mg/kg组,p16蛋白表达值稍高于0.3mg/kg组,差异无统计学意义(P>0.05)。HPPH-PDT0.3mg/kg组和0.45mg/kg组的mtp53蛋白表达值低于HpD-PDT组,p16蛋白表达值高于HpD-PDT组,差异有统计学意义(P<0.05)。结论:HPPH-PDT0.3mg/kg是适宜的HPPH剂量。HPPH-PDT诱导mtp53蛋白表达降低,p16蛋白表达升高。与HpD-PDT相比,HPPH-PDTmtp53蛋白表达更低,p16蛋白表达更高。     

基于真实信号强度的覆盖分析

随着城乡一体化的快速发展,城市大型建筑密度增大,乡镇建筑增多,导致部分场所信号覆盖减弱甚至无信号覆盖.已建成的专网通信系统无法满足不断变化的信号覆盖需求,迫切需要通过新增站点的方式进行扩容补盲.为掌控PDT系统信号覆盖情况,有效实现对PDT系统的扩容补盲,文章研究了一种信号覆盖检测技术,通过整合PDT系统的场强数据达到对信号覆盖的准确分析,为扩容补盲提供有力依据.     

光动力疗法对荷瘤鼠杀伤作用的组织形态学实验研究

目的：探讨光动力疗法恶性肿瘤杀伤作用的机制。方法：应用光动力疗法对接种H22肝癌的40只昆明小鼠作肿瘤杀伤的对比实验研究。结果：通过组织形态学和抑瘤检测证实，光动力疗法能选择性杀伤癌细胞。结论：在治疗后24小时内就可以见到癌细胞出现空泡变性、核固缩、溶解、坏死，随着时间推移坏死更加明显。同时还证实这种光动力疗法对正常细胞和组织没有损伤。     

ALA口服光动力学治疗鼠G22胶质细胞瘤的研究

目的:通过鼠肿瘤动物模型确定ALA口服激光光动力疗法促使肿细胞死亡及凋亡的疗效及光敏药物最佳用药剂量。方法:以不同剂量的ALA口服(20μg/ml、40μg/ml、60μg/ml、120μg/ml、240μg/ml)、以630nm半导体激光200mW/cm2照射,每光斑照射20分钟剂量,照光前及照光后不同时间以肉眼、肿瘤大小测定、病理、电镜、流式细胞仪观察疗效差别并与未作光动力学治疗的空白对照组模型作比较,寻找最佳的光敏药物配合的剂量。结果:随ALA口服剂量增加PDT疗效增加,口服剂量达60mg/kg疗效明显增加能抑止肿瘤生长,肿瘤变暗,质变硬,结痂,60mg/kg,120mg/kg,240mg/kg疗效相似,并不能达到完全的杀除肿瘤,该法作为肿瘤治疗若与HPD-PDT联合使用,可提高疗效,减少HPD剂量,减少避光时间,ALA-PDT可作肿瘤诊断,可避免了HPD光敏诊断的皮肤光毒反应。结论:光动力学治疗脑瘤口服剂量达60mg/kg疗效明显增加,能抑止肿瘤生长,剂量再增加疗效相似,并不能达到完全的杀灭肿瘤。     

光动力治疗对荷瘤小鼠肿瘤淋巴细胞表型的影响

目的:观察光动力治疗(PDT)肿瘤留置对残存肿瘤局部淋巴细胞表型的影响。方法:采用双侧小鼠荷H22移植型肝癌模型。将30只双侧荷瘤小鼠分为3组:PDT组,切除组,对照组。光动力治疗一侧肿瘤后,定量观察残存肿瘤和脾脏内CD4+和CD8+细胞的变化。使用免疫组织化学及图像分析技术。结果:在原位肿瘤内,有极少量的CD4+细胞。在残存肿瘤内,未见CD4+细胞,CD8+细胞呈明显的带状分布,在光动力治疗组,CD8+细胞的密度明显增高,但仍不足以杀死残存肿瘤,其活性也有待进一步研究。     

Impact of RbF and NaF Postdeposition Treatments on Charge Carrier Transport and Recombination in Ga-Graded Cu(In,Ga)Se2 Solar Cells

Two key strategies for enhancing the efficiency of Cu(In,Ga)Se₂ solar cells are the bandgap gradient across the absorber and the incorporation of alkali atoms. The combined incorporation of Na and Rb into the absorber has brought large efficiency gains compared to Na-containing or alkali-free layers. Here, transient absorption spectroscopy is employed to study the effect of NaF or combined NaF+RbF postdeposition treatments (PDT) on minority carrier dynamics in different excitation volumes of typical composition-graded Cu(In,Ga)Se₂ solar cells. Electron lifetimes are found to be highly dependent on the film composition and morphology, varying from tens of nanoseconds in the energy notch to only ≈100 ps in the Ga-rich region near the Mo-back contact. NaF PDT improves recombination lifetimes by a factor of 2–2.5 in all regions of the absorber, whereas the effectiveness of the RbF PDT is found to decrease for higher Ga-concentrations. Electron mobility measured in the absorber region with large grains is promoted by both alkali PDTs. The data suggest that NaF PDT passivates shallow defect states (Urbach tail) throughout the Cu(In,Ga)Se₂ film (including the interior of large grains), whereas the additional RbF PDT is effective at grain boundary surfaces (predominantly in regions with medium to low Ga-concentrations).