PPI协议分析

使用软件监听串口,记录Step7-Micro/SIN与S7-200 PLC通讯的PPI协议报文,参照PROFIBUS总线通讯协议,分析出PPI协议报文关键帧格式,实现读、写、锁定、解锁、连接测试、开停PLC等常用功能,可用于开发基于S7-200系列PLC、使用PPI协议进行通讯的上位机程序或轻量级的现场设备.     

Applying Protein–Protein Interactions and Complex Networks to Identify Novel Genes in Retinitis Pigmentosa Pathogenesis

Retinitis Pigmentosa (RP) is a hereditary retinal disorder that causes the atrophy of photoreceptor rod cells. Since individual defective genes converge on the same disease, we hypothesized that all causal genes of RP belong in a complex network. To explore this hypothesis, we conducted a gene connection analysis using 161 genes attributed to RP, compiled from the Retinal Information Network, RetNet. We then examined the protein interaction network (PIN) of these genes. In line with our hypothesis, using STRING, we directly connected 149 genes out of the recognized 159 genes. To uncover the association between the PIN and the ten unrecalled genes, we developed an algorithm to pinpoint the best candidate genes to connect the uncalled genes to the PIN and identified ten such genes. We propose that mutations within these ten genes may also cause RP; this notion is supported by analyzing and categorizing the known causal genes based on cellular locations and related functions. The successful establishment of the PIN among all documented genes and the discovery of novel genes for RP strongly suggest an interconnectedness that causes the disease on the molecular level. In addition, our computational gene search protocol can help identify the genes and loci responsible for genetic diseases, not limited to RP.     

高温花生粕蛋白提取及功能性质的研究

以高温花生粕为原料,进行了主要成分的测定,采用正交优化花生蛋白的提取工艺,并对所提花生分离蛋白进行功能性质的测定与研究。花生蛋白提取的最佳工艺为:温度60℃,pH值为10,料水比(g∶mL)为1∶10,浸提时间为2h;花生分离蛋白的功能性质较好,应用价值高。     

豌豆分离蛋白对小麦粉面团特性及挂面品质的影响

研究豌豆分离蛋白(PPI)添加量对小麦粉面团蛋白质组成、粉质特性、拉伸特性和对挂面断裂强度、烹煮特性及感官品质的影响。结果表明:添加PPI后面团中23.8~100.1 ku的蛋白条带明显加深;当添加量为9.0%时,面团吸水率提高8.0%,稳定时间延长36.6%,拉伸阻力提高83.2%,拉伸比增大176.2%,说明PPI的添加能够提高面团的筋力;PPI添加量达到7.0%时,挂面最佳烹煮时间降低11.8%,生挂面断裂强度提高34.0%,同时熟挂面的硬度、胶着性和咀嚼度有不同程度的提高。感官品评表明,添加3.0%~7.0%PPI制备的挂面与普通挂面无显著差异。     

换流阀用与直流断路器用压接型IGBT器件差异分析

压接型IGBT器件以其耐受电压高、电流密度大、控制功率低、开关速度快以及双面散热等优势成为柔性直流输电系统中换流阀和直流断路器选用的理想器件。由于应用装备的差异,换流阀和直流断路器对于压接型IGBT器件分别提出了低通态压降和高短路关断能力的要求。分析了换流阀用压接型IGBT器件和直流断路器用压接型IGBT器件外部和内部电流、电压、温度和压力的差异,总结了两种器件应用过程中可能存在的主要失效原因,提出了封装设计中需要重点考虑的技术问题。     

Essential Protein Prediction Based on Shuffled Frog-Leaping Algorithm

Essential proteins are integral parts of living organisms.The prediction of essential proteins facilitates to discover disease genes and drug targets.The prediction precision and robustness of most of existing identification methods are not satisfactory.In this paper,we propose a novel essential proteins prediction method(EPSFLA),which applies Shuffled frog-leaping algorithm(SFLA),and integrates several biological information with network topological structure to identify essential proteins.Specifically,the topological property and several biological properties (function annotation,subcellular localization,protein complex,and orthology) are integrated and utilized to weight protein-protein interaction networks.Then the position of a frog is encoded and denotes a candidate essential protein set.The frog population continuously evolve by means of local exploration and global exploration until termination criteria for algorithm are satisfied.Finally,those proteins contained in the best frog are regarded as predicted essential proteins.The experimental results show that EPSFLA outperforms some well-known prediction methods in terms of various criteria.The proposed method aims to provide a new perspective for essential protein prediction.     

用于成对PPI网络比对的分治与整合算法

生物网络比对是分析不同生物间进化关系的重要手段,它可以揭示不同物种间的保守功能并为物种间的注释转移提供重要信息。网络比对与子图同构类似,是一个NP-hard问题。本文提出了一种新的分治与整合策略的生物网络比对算法。首先进行模块划分,并根据已有的比对信息计算模块相似性;然后根据模块间结点的子比对获取候选结果集,最终通过超图匹配获得比对结果。使用已有的比对信息的集体行为预估模块间的相似性,大大提高了模块匹配的效率。基于路径和结点的得分函数保证了模块内结点的相似性。对于不同网络间结点的相似性,分别从结点自身和结点间的差异进行相似性判断。与现有算法相比,本文算法在生物和拓扑指标上均表现最佳。     

基于空间映射的蛋白质相互作用网络链接预测算法

蛋白质间的相互作用预测问题本质上是复杂网络的链接预测问题。到目前为止,已经有很多方法用于链接预测,这些方法要么只考虑拓扑信息,要么只考虑蛋白质相互作用网络内部的交互信息,但是仅考虑一种信息来预测蛋白质的交互信息是远远不够的。因此提出了一种新方法:将蛋白质相互作用网络看作是一个有权图,根据网络中两节点的拓扑结构和属性信息,分别计算它们的拓扑相似度和属性相似度来预测它们之间是否存在链接关系。在两种相似度平衡方面,考虑基于空间映射的方法,将它们独立地映射到另一空间,并且使它们分别映射的空间尽量相近,从而使得拓扑信息、属性信息有机融合。实验结果表明,提出的算法具有较好的准确率和良好的生物统计特性。     

卷染机中央控制系统

针对多台卷染机实时监测与控制的实际需求,根据西门子S7-200系列PLC的特点设计了一套成本低、扩展性强的基于串口服务器的工业以太网设备联网方案.计算机通过PPI协议同时对多台S7-200 PLC混合存储区多组数据的一次读写技术,实现集中控制多台卷染机,检测设备状态、上传和下载配方和显示主要工艺参数图表的功能.通过调试检测,系统能够平稳的长时间运行,完全可以满足实际使用需要.     

Structural Characterization of Receptor–Receptor Interactions in the Allosteric Modulation of G Protein-Coupled Receptor (GPCR) Dimers

G protein-coupled receptor (GPCR) oligomerization, while contentious, continues to attract the attention of researchers. Numerous experimental investigations have validated the presence of GPCR dimers, and the relevance of dimerization in the effectuation of physiological functions intensifies the attractiveness of this concept as a potential therapeutic target. GPCRs, as a single entity, have been the main source of scrutiny for drug design objectives for multiple diseases such as cancer, inflammation, cardiac, and respiratory diseases. The existence of dimers broadens the research scope of GPCR functions, revealing new signaling pathways that can be targeted for disease pathogenesis that have not previously been reported when GPCRs were only viewed in their monomeric form. This review will highlight several aspects of GPCR dimerization, which include a summary of the structural elucidation of the allosteric modulation of class C GPCR activation offered through recent solutions to the three-dimensional, full-length structures of metabotropic glutamate receptor and γ-aminobutyric acid B receptor as well as the role of dimerization in the modification of GPCR function and allostery. With the growing influence of computational methods in the study of GPCRs, we will also be reviewing recent computational tools that have been utilized to map protein–protein interactions (PPI).