A prerequisite for effective pest risk management in food is the unbiased interpretation of results obtained by various detection methods. In this study we compared the sensitivity of filth flotation tests, sieving and heat extraction in Tullgren–Berlese funnels for detecting insect contaminants. Samples of wheat grain, flour and semolina were contaminated with eggs, juveniles and adults of Tribolium castaneum, and eggs or larvae of Ephestia kuehniella. Calibration methods were applied for every detection method, and total and sample recoveries and detection limits were calculated for each method, food substrate and contaminant type. The tested contaminants were not detected on a qualitative level by any single technique, instead a combination of techniques was necessary for detection. Sieving was the method with the highest total recoveries, ranging from 90 to 100%. Filth flotation was a uniquely effective for egg detection, with total recoveries ranging from 65 to 95%. The extraction of adults and larvae of both species in Tullgren–Berlese funnels failed in semolina and flour, and was of very limited success in grain. The detection limits for sieving were from 1 to 16 contaminants/kg commodity. The detection limits for filth flotation were from 224 to 508 eggs, and 58 to 507 adults or larvae/kg commodity. The sample recoveries were usually influenced by sample size, species, stadium and their interactions, and indicated how to optimize method protocols. The calibration of methods provided estimates of contaminant densities different from those obtained without calibration. Our work revealed that some currently used methods are not sensitive enough to detect all stages of insect pests, or in some cases, low levels of pest infestation. This lack of sensitivity potentially enables the infested cereal food product to continue down the food processing chain even after laboratory inspection. 相似文献
Three varieties of paddy rice with and three varieties without lipoxygenase (LOX) isoenzymes were stored at room temperature, in dark conditions for 42 months. Insect damage and viability were investigated during that time. Varieties without LOX-1,2 and their offspring were screened with a rapid method of estimating LOX isoenzymes, and subjected to an accelerated-ageing experiment. The results indicated that in varieties without LOX-1,2, after long storage, rice quality was normal, but for varieties with LOX-1,2, rice quality deteriorated. With increase of storage time, the germination rate of the seed of most varieties decreased greatly except for varieties without LOX-1,2 where there was almost no change in the germination rate. In varieties without LOX-1,2 and their offspring, after an accelerated ageing experiment the germination rate of some varieties changed slowly, but with others, the germination rate changed quickly. This indicated that a definitive factor, which influenced the life of seeds, may be the loss of LOX-1,2. During storage, there was also a significant difference in the degree of insect damage. In varieties without LOX-3 insect damage was lower, but in varieties with LOX-3 insect damage was higher. Therefore, the absence of LOX-3 may be very important in insect resistance in rice grain. 相似文献
Aflatoxin level in 300 farmers’ stores in four agro-ecological zones in Benin, a west African coastal country, were determined over a period of 2 years. At sampling a questionnaire was used to evaluate maize storage practices. Farmers were asked what storage structure they used, their storage form, storage period, pest problems in storage and what was done against them. Beninese farmers often changed their storage structures during the storage period, transfering the maize from a drying or temporary store to a more durable one. Most of the farmers complained about insects damaging stored maize. Often, storage or cotton insecticides were utilized against these pests. Regression analysis identified those factors that were associated with increased or reduced aflatoxin.
Maize samples in the southern Guinea and Sudan savannas were associated with higher aflatoxin levels and the forest/savanna mosaic was related to lower toxin levels. Factors associated with higher aflatoxin were: storage for 3–5 months, insect damage and use of Khaya senegalensis-bark or other local plants as storage protectants. Depending on the agroecological zone, storage structures that had a higher risk of aflatoxin development were the “Ago”, the “Secco”, the “Zingo” or storing under or on top of the roof of the house. Lower aflatoxin levels were related to the use of storage or cotton insecticides, mechanical means or smoke to protect against pests or cleaning of stores before loading them with the new harvest. Fewer aflatoxins were found when maize was stored in the “Ago” made from bamboo or when bags were used as secondary storage containers. 相似文献
In this study frozen fillets of sardine (Sardina pilchardus) were used to make marinades. The marinating process was performed in 7% acetic acid and 14% sodium chloride in barrels.
The fish:solution ratio was (1.5:1). After the marination process, sardine fillets were packed in glass jars in two different
formulations fish:solution ratio of (1.5:1); the first formulation contained 2% acetic acid and 4% sodium chloride with tomato
sauce and spices and the other was 2% citric acid and 4% sodium chloride with lemon and also the same spices. Pasteurization
process had been applied on half of the jars at 70 °C for 20 min. Chemical, enzymatical and textural changes during marination
and 6 months storage period of sardine marinades were determined. The results obtained in proteolytic activities correlated
well with the observed texture measurements according to time of storage. A decrease in the histidine content and an increase
in glutamic acid and aspartic acid contents of marinated sardines were determined. Histamine levels were lower than the toxic
limit (100 mg/kg) during the marination and storage period of sardine marinades. 相似文献
The objective of this study was to examine the relation between storage period and isoflavone concentration and composition in soybean [Glycine max (L.) Merrill] seeds. Total isoflavones varied from 470.0 to 878.1 µg g–1 over the three cropping years and there were significant differences between the soybean cultivars. Generally, the malonyl conjugates were in higher concentrations than were the other compounds, and the individual isoflavones varied with each cropping year. The Myeongjunamul (1998: 936.7 µg g–1, 1999: 727.0 µg g–1, 2000: 807.8 µg g–1) cultivar had the highest concentration, on average. Regarding changes in isoflavones with storage period, the concentration decreased by 91.7 to 171.1 µg g–1. Malonyldaidzin and malonylgenistin concentrations also decreased, and the concentration of glucosides increased slightly over the 3 years. Acetyldaidzin, acetylglycitin and glycitein could not be detected or were only found in very small concentrations. Pureun (–66.2 µg g–1) and Myeongjunamul (–77.4 µg g–1) cultivars showed little variation in isoflavone levels, whereas the concentration in Muhan (–295.4 µg g–1) decreased markedly on average over the three storage years. There were also significant differences between the cultivars. Our study suggests that it might be feasible to improve the storage methods of soybeans to preserve them for longer periods, which would have benefits for both their import and their export.S.H. Kim and W.S. Jung contributed equally to this work. 相似文献