中国对虾组织细胞中酚氧化酶活力的研究

运用酶细胞化学技术，显示中国对虾（Fenneropenaeus chinensis）淋巴器、皮肤、鳃、肝胰腺、中肠育囊和中肠这几种组织细胞中的酚氧化酶活性，在透射电镜下观察各组织中细胞内酚氧化酶的活性部位；观察在受病毒感染的细胞内酚氧化酶对病毒的作用。结果表明，在健康对虾的组织细胞中，基本不表现酚氧化酶活性，而在受病毒感染的对虾中，不同的组织表现不同程度的酚氧化酶活性。其中，尤以淋巴器和皮肤的细胞中酚氧化酶活性最强，在淋巴器和皮肤这两个组织的细胞中，都可看到酚氧化酶对病毒结构的破坏作用。中国对虾淋巴器具有对血淋巴中有害异物进行过滤的作用，酚氧化酶在这一过程中发挥了重要作用。     

Inhibition of Respiratory Syncytial Virus Infection by Small Non-Coding RNA Fragments

Respiratory syncytial virus (RSV) causes acute lower respiratory tract infection in infants, immunocompromised individuals and the elderly. As the only current specific treatment options for RSV are monoclonal antibodies, there is a need for efficacious antiviral treatments against RSV to be developed. We have previously shown that a group of synthetic non-coding single-stranded DNA oligonucleotides with lengths of 25–40 nucleotides can inhibit RSV infection in vitro and in vivo. Based on this, herein, we investigate whether naturally occurring single-stranded small non-coding RNA (sncRNA) fragments present in the airways have antiviral effects against RSV infection. From publicly available sequencing data, we selected sncRNA fragments such as YRNAs, tRNAs and rRNAs present in human bronchoalveolar lavage fluid (BALF) from healthy individuals. We utilized a GFP-expressing RSV to show that pre-treatment with the selected sncRNA fragments inhibited RSV infection in A549 cells in vitro. Furthermore, by using a flow cytometry-based binding assay, we demonstrate that these naturally occurring sncRNAs fragments inhibit viral infection most likely by binding to the RSV entry receptor nucleolin and thereby preventing the virus from binding to host cells, either directly or via steric hindrance. This finding highlights a new function of sncRNAs and displays the possibility of using naturally occurring sncRNAs as treatments against RSV.     

模块化免疫神经网络模型在计算机病毒分类检测中的应用

结合神经网络优化、聚类的特点、免疫网络识别自己、非己和抗体处理抗原的机理以及遗传算法中适者生存和优胜劣汰的原理,探讨一种基于神经网络方法下的新的计算机病毒分类检测模型,该模型能动态实现系统处理信号的分类,而且对每类信号也给出了综合有效的处理方法,其利用神经网络和免疫网络相互作用的规律,最终形成一个动态的、自适应的、分布式的模型。该模型可有效地解决病毒分类检测问题,有较大潜力和发展空间。     

抗新型冠状病毒抗体样本盘在其胶体金测试卡质量评价中的应用

目的 建立抗新型冠状病毒(SARS-CoV-2)抗体样本盘,并应用于SARS-CoV-2抗体胶体金测试卡的质量评价.方法 采用新型冠状病毒肺炎(Corona Virus Disease 2019,COVID-19)康复者恢复期经病毒灭活的血浆样本建立SARS-CoV-2抗体样本盘,并进行优化.收集国内3个厂家(厂家S、...     

针对网络机房ARP欺骗攻击的研究与防范

分析了ARP安全漏洞及病毒攻击原理。探讨了在局域网内的主机和网络变换设备等应多方面采取防护措施和应对策略。     

基于智能扫描的病毒监视器的研究

详细阐述了当今病毒发展的新特性以及在当前环境下传统的基于病毒特征码的病毒扫描器的不足。在按照病毒种类对各类病毒详细分析之后总结出病毒的一系列行为特征以及相应的典型代码结构。提出了一种全新的基于智能扫描的病毒监视器的体系结构和工作原理，并对它今后的发展进行了展望。     

Determination of virus abundance, diversity and distribution in a municipal wastewater treatment plant

Procedures to extract and count sludge viruses-like particles from municipal sewage treatment plant were optimized by epifluorescence microscopy using SYBR Green I as a stain. The highest indigenous virus yields from the bulk of the anaerobic digestion sludge and influent (solid) were obtained by utilizing 10 mM sodium pyrophosphate as eluant solution with vortex and 1 min of sonication. The use of 1× phosphate buffered saline as eluant with vortex and 1 min of sonication yields highest indigenous virus from activated sludge. The efficiency of extracting indigenous viruses by sodium pyrophosphate-ultrasound treatment was about 62% of the extractable virus particles from activated sludge and 87% for anaerobic digestion sludge, respectively. Samples treated with DNase had decreased, but not significant, virus counts, suggesting a minor effect of extracellular DNA on virus count. Following the optimized procedure, we investigated the abundance and diversity of virus particles in the wastewater stream of a municipal treatment plant. The concentrations of virus particles ranged from 0.28 × 10⁹ ml⁻¹ to 27.04 × 10⁹ ml⁻¹. Transmission electron microscopy (TEM) showed a high variety of virus morphotypes in sludge. Pulsed-field gel electrophoresis (PFGE) revealed a diverse and dynamic viral community in different stages of the system with genome sizes ranging from 33 kb to >350 kb with most of the viral DNA in the 30-80 kb and 200-350 kb size ranges. Collectively, our study suggested that indigenous viruses are abundant and dynamic in the municipal wastewater treatment system and may play an important role in functioning of the system.     

浅谈计算机信息安全与密码学

信息安全是为了让社会安宁,不让网上信息被泄露,黑客入侵,计算机病毒传播等,面临着政治、经济、外交、科技、教育和意识形态等方面的总体网络斗争,为我国的信息安全构筑起可靠屏障。     

计算机网络安全技术探讨

本文论述了计算机网络安全的内容和解决方案,并对网络防火墙技术、加密技术和网络防病毒技术进行了初步探讨。