STN7 Kinase Is Essential for Arabidopsis thaliana Fitness under Prolonged Darkness but Not under Dark-Chilling Conditions

Reversible phosphorylation of photosystem II light harvesting complexes (LHCII) is a well-established protective mechanism enabling efficient response to changing light conditions. However, changes in LHCII phosphorylation were also observed in response to abiotic stress regardless of photoperiod. This study aimed to investigate the impact of dark-chilling on LHCII phosphorylation pattern in chilling-tolerant Arabidopsis thaliana and to check whether the disturbed LHCII phosphorylation process will impact the response of Arabidopsis to the dark-chilling conditions. We analyzed the pattern of LHCII phosphorylation, the organization of chlorophyll–protein complexes, and the level of chilling tolerance by combining biochemical and spectroscopy techniques under dark-chilling and dark conditions in Arabidopsis mutants with disrupted LHCII phosphorylation. Our results show that during dark-chilling, LHCII phosphorylation decreased in all examined plant lines and that no significant differences in dark-chilling response were registered in tested lines. Interestingly, after 24 h of darkness, a high increase in LHCII phosphorylation was observed, co-occurring with a significant F_V/F_M parameter decrease. The highest drop of F_V/F_M was detected in the stn7-1 line–mutant, where the LHCII is not phosphorylated, due to the lack of STN7 kinase. Our results imply that STN7 kinase activity is important for mitigating the adverse effects of prolonged darkness.     

通过液位波动来检测搅拌器的运行故障

通过仪表检测液位波动的方式来间接检测搅拌器的运行状态和故障,从而解决在高速搅拌轴无法安装测量仪表的问题。     

浅谈PLC在石油钻机中的应用

随着电驱动钻机在油田中的应用,PLC成为其控制的核心讲述了利用SIMATIC S7—300进行系统集成的设计方法。     

宽厚板主体设备定尺剪切工艺控制优化

在简述轧钢部的主体设备定尺剪问题的基础上,论述通过定尺剪控制系统软件优化修改来解决生产中存在的生产序列瓶颈问题,从控制角度对西门子S7—400的程序进行了多处改进和优化。     

基于WinCC6．0及S7-200PLC的材料分拣监控系统设计

本文采用西门子s7-200系列PLC和winCC监控软件相结合的控制模式,实现了材料、分拣的全自动监视与控制。S7-200PLC和以WinCC为OPC客户端的监控软件之间的通讯采用s7—200PCACCESS作为OPC服务器实现。经过对材料分拣装置的实时监控,证明系统稳定性和准确度较好,能满足监控系统的需要。     

基于S7-400与WINCC6．0的传动故障诊断系统

本文主要通过S7-400和WINCC60的使用介绍传动故障诊断系统的开发设计,通过系统的实际应用,使大家了解传动诊断系统的功能和作用对现代化高节奏生产企业的重要性。     

多媒体系列标准MPEG的发展研究

通过对MPEG－1到MPEG－21发展过程的分析，详细论述了MPEG系列标准的技术原理及应用领域；并对MPEG－4，MPEG－7和MPEG－21标准的主要技术进行了重点研究。     

AT91SAM7S64芯片I2C(TWI)接口应用开发

较为详细地介绍AT91SAM7S64芯片的I2C接口控制器中外围数据控制器(PDC)的使用方式,并将其与AT24C04之间的通信作为实例给出完整的应用开发流程和软硬件详细描述。该方法的可行性已经为工程实践和实际产品所证实。该操作也可推广到其他AT91SAM7S系列ARM处理器与其他I2C接口器件间的通信。并对其他拥有外围数据控制器的接口开发有一定的借鉴意义。     

基于PLC控制的气动搬运机械手系统设计

介绍了某种工业用气动搬运机械手的组成结构、功能和动作流程。通过PLC控制气动系统完成指定动作,并通过S7-200 脉冲输出方式,应用MAP库编程控制步进电机,实现机械手的精确运动控制以及气动机械手的设计功能。     

长脉冲激光沉积原位生成YBaCuO超导膜

用长脉冲激光(脉冲宽度150μs,波长1.06μm)辐照高温烧结的YBa_2Cu_3O_(7-x)靶,在6Pa的氧气压强下,巳在(100)YSZ单晶衬底上原位生成YBa_2Cu_(?)O_(7-x)超导膜。衬底置于750℃的加热器上,衬底与靶之间的距离5cm,用该法制得的薄膜光亮坚实,正常态呈金属性,零电阻温度为84.7K。用XRD和SEM对薄膜进行了分析研究。