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101.
Altschuh Daniele; Tessier Daniel C.; Vernet Thierry 《Protein engineering, design & selection : PEDS》1994,7(6):769-776
The two main catalytic residues Cys25 and Hisl59 of the monomericcysteine protease papain are located on different walls of acleft formed by two domains. This topology suggests a possiblerelationship between relative domain organization and catalyticmechanism. The effect on enzymatic parameters of structuralmodifications at various locations of the twodomain interfaceof papain was examined by individual or double replacementsby Ala of pairs of interacting residues. Most modificationshad no effect on enzyme activity. However, the enzyme's substrateturnover (kcat) decreased following simultaneous alterationof the two most conserved residues, forming an apolar contactlocated 15 Å away from the active site. The pH activityprofile of the double mutant was unchanged, indicating a conservedionization state of the active site thiolate-imidazolium ionpair. This state is strongly dependent on the distance separatingthe two residues, thus suggesting that the active site geometryhas not been significantly altered. Efficient enzymatic activityin papain requires more than a correct active site geometryand is influenced by domain packing properties in a region remotefrom the active site. 相似文献
102.
采用精确的有限元非线性分析方法对小高跨比单层单跨实腹门式刚架的平面内弹性整体稳定性进行了大量的参数分析,指出在梁上均布荷载作用下,当柱梁高跨比小于某一数值时,一般不发生传统理论所认为的反对称失稳模式,而是以对称失稳模式为主。给出了修正的柱计算长度系数,并与规范给出的数值进行了对比,认为这种对称失稳模式更不利于门式刚架的整体稳定,应该引起足够的重视。 相似文献
103.
104.
Building a knowledge-based system is like developing a scientific theory. Although a knowledge base does not constitute a theory of some natural phenomenon, it does represent a theory of how a class of professionals approaches an application task. As when scientists develop a natural theory, builders of expert systems first must formulate a model of the behavior that they wish to understand and then must corroborate and extend that model with the aid of specific examples. Thus there are two interrelated phases of knowledge-base construction: (1) model building and (2) model extension. Computer-based tools can assist developers with both phases of the knowledge-acquisition process. Workers in the area of knowledge acquisition have developed computer-based tools that emphasize either the building of new models or the extension of existing models. The PROTÉGÉ knowledge-acquisition system addresses these two activities individually and facilitates the construction of expert systems when the same general model can be applied to a variety of application tasks. 相似文献
105.
介绍了管环缝射线透照方式,以及环缝单壁外透法和环缝双壁单影法100%射线透照检查一次透照长度及分段次数的计算。 相似文献
106.
试验模态分析技术的进展 总被引:5,自引:0,他引:5
本文对试验模态分析技术的进展,特别是80年代发展起来的有关新技术进行了综合评述。主要内容有:试验激振技术、频响函数估计方法、试验测试设备、以及各种模态识别方法,包括单输入/多输出和多输入/多输出识别的时域和频域方法。文中强调了试验模态分析技术各环节之间的相互联系、相互影响,对于80年代已成为模态识别主流的多输入/多输出各种方法(包括作者发展的几种方法),用统一方法作了描述。最后提出了若干值得进一步研究的方向。 相似文献
107.
Eugene Fiume† 《Computer Graphics Forum》1995,14(1):47-58
The main preoccupations of research in computer-aided geometric design have been on shape-specification techniques for polynomial curves and surfaces, and on the continuity between segments or patches. When modelling with such techniques, curves and surfaces can be compressed or expanded arbitrarily. There has been relatively little work on interacting with direct spatial properties of curves and surfaces, such as their arc length or surface area. As a first step, we derive families of parametric piecewise polynomial curves that satisfy various positional and tangential constraints together with arc-length constraints. We call these curves isometric curves. A space curve is defined as a sequence of polynomial curve segments, each of which is defined by the familiar Hermite or Bézier constraints for cubic polynomials; as well, each segment is constrained to have a specified arc length. We demonstrate that this class of curves is attractive and stable. We also describe the numerical techniques used that are sufficient for achieving real time interaction with these curves on low-end workstations. 相似文献
108.
本文分析了使用计数的原理来测量长度、转速的仪器,其误差产生的原因,并结合G3K-3型高准确度长度测量仪,着重介绍了消除误差的方法. 相似文献
109.
Oliver W. W. Yang 《Performance Evaluation》1995,23(3):261-284
This paper analyzes and compares the slotted time operation and the non-slotted time operation of a singleserver system with deterministic service time. These two operations are commonly used to model discrete service time systems in computer or digital communications. But due to the similarity in their operations and performance, the two models may be mixed up with each other. This paper examines by means of queueing analysis the performance of a few classes of systems that differ in the service order of their customers. The means and variances of the queue lengths, waiting times and interdeparture times of FCFS systems using the slotted time operations are first obtained from their respective LST and generating function equations. These are then used for comparison with those in non-slotted time systems. The results show that although the slotted time operation in the FCFS systems can be approximated by the non-slotted time operations under heavy traffic condition, the performances under other regions and service disciplines (e.g., the LCFS and the priority systems) may deviate significantly. They must be properly adjusted if one wishes to use the simpler equations of the non-slotted time operations to approximate the slotted time operations. The comparison graphs provided in this paper supply adjustments guidelines for the careful designers. 相似文献
110.
运用HerbstFW公式原理对国产碱性条件进行了大量试验,建立了焊接线能量(E),焊条熔化长度(lE),焊道长度(lR)的关系式:E=808D1.8lE/lR。并应用线算图和SH-CCT图对该式施焊结果验证,热影响区的金相组织,硬度基本一致。因此,可以应用焊道长度校核与控制线能量。 相似文献