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991.
992.
Complete fusion of two selected cells allows for the creation of novel hybrid cells with inherited genetic properties from both original cells.Alternatively,via fusion of a selected cell with a selected vesicle,chemicals or genes can be directly delivered into the cell of interest,to control cellular reactions or gene expression.Here,we demonstrate how to perform an optically controlled fusion of two selected cells or of one cell and one vesicle.Fusion is mediated by laser irradiating plasmonic gold nanoparticles optically trapped between two cells (or a vesicle and a cell) of interest.This hot-particle-mediated fusion causes total mixing of the two cytoplasms and the two cell membranes resulting in formation of a new hybrid cell with an intact cell membrane and enzymatic activity following fusion.Similarly,fusion between a vesicle and a cell results in delivery of the vesicle cargo to the cytoplasm,and after fusion,the cell shows signs of viability.The method is an implementation of targeted drug delivery at the single-cell level and has a great potential for cellular control and design. 相似文献
993.
The effect of transition metal ions(M~(2+)=Mn~(2+),Ni~(2+),Co~(2+),Cu~(2+)) on the chemical synthesis of polyaniline(PANI) used as a platinum-free counter electrode(CE) in dye-sensitized solar cells(DSSCs) was investigated.PANI was synthesized by co-polymerization of aniline in the presence of different transition metal ions by using potassium dichromate in acidic medium. It was found that the ion doping of PANI showed a certain catalytic activity for the regeneration of traditional iodide/triiodide(I~-/I_3~-) redox couples. The power conversion efficiency(η) of PANI CEs doped with Mn~(2+),Ni~(2+),Co~(2+) (4.41%, 2.36% and 2.10%, respectively) were higher than 1.94%, the value measured for PANI CE without doping. Doping with Cu~(2+)decreased the power conversion efficiency of PANI CE(PANI-Cu~(2+) η = 1.41%). The electrical properties of the PANI, PANI-Ni~(2+), PANI-Co~(2+),PANI-Mn~(2+) and PANI-Cu~(2+) were studied by cyclic voltammetry(CV), impedance(EIS), and Tafel polarization curve. The experimental results confirmed that PANI was affected by the doping of different transition metal ions(M~(2+)=Mn~(2+),Ni~(2+),Co~(2+),Cu~(2+)). These results indicate a potential application of ion doped PANI as counter electrode in cost-effective DSSCs. 相似文献
994.
The adoption of Th fuel in fast reactors is being reconsidered due to the potential favorable impact on actinide waste management and resource availability. A closed Th cycle leads to an actinide inventory with lower radiotoxicity and heat load for the first several thousands of years. Due to the typically low TRansUranic (TRU) Conversion Ratio (CR), Th can also be advantageous to expedite the consumption of legacy TRU. One of the main obstacles to the implementation of Th is the highly radioactive recycled fuel which requires remote handling under heavy shielding, inevitably penalizing economics and challenging conventional pin-based fuel manufacturing. From this perspective, the development of liquid-fuelled reactors, with Molten Salt Reactors regarded as the most promising, appears particularly attractive as fuel handling would be greatly simplified. The present paper investigates the fuel cycle performances of the reference GEN-IV Molten Salt Fast Reactor (MSFR) in terms of isotope evolution, radiotoxicity generation and safety-related parameters. Similarly to most MSR concepts proposed in the past, the MSFR is based on the fluoride molten salt technology, but it features the novelty of a fast neutron spectrum. Calculations are performed using state-of-the-art equilibrium-cycle methodologies, i.e., the ERANOS-based EQL3D procedure developed at the Paul Scherrer Institut and extended to the simulation of the MSFR. Selected results have been benchmarked with the Monte Carlo code PSG2/SERPENT. These results have also been used for the assessment of a diffusion module based on the COMSOL multi-physics toolkit, which is the subject of current studies aimed at efficiently simulating the peculiar MSFR transient behavior. 相似文献
995.
王啟元 《徐州建筑职业技术学院学报》2012,12(2):1-3
用自平衡法进行桩基检测时,荷载箱的埋设位置决定检测数据的真实性.现行自平衡点计算公式不考虑桩顶荷载作用,影响测试结果.为了避免因为平衡点计算精度影响测试结果,根据天津市文化中心交通枢纽工程实际情况,考虑桩顶上部回填级配碎石对平衡点的影响,修正既有平衡点计算公式.运用修正后的公式,计算天津市文化中心交通枢纽工程桩平衡点,并与设计值进行了比较,吻合较好. 相似文献
996.
Artificial extracellular matrix for embryonic stem cell cultures: a new frontier of nanobiomaterials
AbstractNanobiomaterials can play a central role in regenerative medicine and tissue engineering by facilitating cellular behavior and function, such as those where extracellular matrices (ECMs) direct embryonic stem (ES) cell morphogenesis, proliferation, differentiation and apoptosis. However, controlling ES cell proliferation and differentiation using matrices from natural sources is still challenging due to complex and heterogeneous culture conditions. Moreover, the systemic investigation of the regulation of self-renewal and differentiation to lineage specific cells depends on the use of defined and stress-free culture conditions. Both goals can be achieved by the development of biomaterial design targeting ECM or growth factors for ES cell culture. This targeted application will benefit from expansion of ES cells for transplantation, as well as the production of a specific differentiated cell type either by controlling the differentiation in a very specific pathway or by elimination of undesirable cell types. 相似文献
997.
《International Journal of Hydrogen Energy》2021,46(67):33523-33540
The metal-supported solid oxide fuel cell (MS-SOFC) is of current research interest in the clean energy field due to its high performance, quick start-up, thermal cycle stability, and lower raw material cost compared to the conventional cermet-based SOFC. To efficiently operate a MS-SOFC using complex hydrocarbon and logistic fuels, it is required to introduce an internal reforming catalyst within the anode metal scaffold. This review article discusses some examples of the performance of MS-SOFCs under hydrocarbon and logistic fuels with and without an additional reforming catalyst. We also discuss the performance improvement of conventional cermet-based SOFCs by adding reforming catalysts via the infiltration method. This information can be directly applied to future MS-SOFC applications. Furthermore, this review article proposes possible novel methods such as direct precursor infiltration, catalyst-anode premixing, and atomic layer deposition methods to introduce the reforming catalyst into a MS-SOFC for improving its initial electrochemical performance and long-term stability under hydrocarbon and logistics fuel. 相似文献
998.
In the nuclear industry, safely managing spent fuels discharged from PWRs (pressurized water reactors) is an ongoing challenge. In this paper, a synergistic coupling of innovative small long‐cycle PWRs and advanced sodium‐cooled fast reactors is considered to reduce the accumulated TRUs (transuranics) by transmuting them with electricity production. In the coupling strategy, the innovative small PWRs employing UO2–ThO2 and fully ceramic micro‐encapsulated fuels are used to deeply burn TRUs from commercial PWRs, while advanced SFRs (sodium‐cooled fast reactors) with actinide recycling are designed to further transmute the TRUs discharged from innovative small PWRs. This work focuses on the core physics analysis of new SFR burner cores using different TRU feeds discharged from small PWRs. Additionally, quasi‐static reactivity balance analyses are performed to understand the safety of the SFR burner cores. The mass flows of TRUs in the nuclear park, which is composed of PWRs, small long‐cycle PWRs, and SFR burners, are analyzed to evaluate TRU inventory reduction. The results of this study show that the advanced SFR burners with all the TRU feed types discharged from the small long‐cycle PWRs have a high TRU consumption rate. They satisfy all of the conditions for self‐controllability under unprotected accidents with a reasonable number of control rods. This coupling strategy requires ~35% less power in conjunction with the advanced SFR burners in the nuclear park and increases the support ratio of SFR burners by ~42% than does the coupling of commercial PWRs and SFR burners. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
999.
Margaret H. Kurth Jesse M. Keenan Mehrdad Sasani 《Building Research & Information》2019,47(4):480-492
ABSTRACTInitiatives to operationalize the concept of resilience in the building industry are rapidly emerging. The concept of resilience has introduced a way to explore solutions to some important problems in the building industry. However, much of the work that has taken place to date represents activities generally assigned to risk management, which is discussed as being inherently insufficient for sustaining the functions of the built environment under stresses. This commentary considers the opportunities and limitations for mainstreaming resilience into building industry processes and actors. Barriers include indeterminate analytical meaning, event and performance uncertainty, immature regulatory standards setting, and untested enterprise economics. Further, the multiple outcomes of recovery and the relationship between building recovery and adaptation are discussed and, along with economics of resilience investments, a research need highlighted. A simple heuristic is presented to illustrate the complement of resilience to risk management and advance the integration of resilience into existing industry workflows. 相似文献
1000.