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91.
A series of experiments have been conducted using a recombinant baculovirus/insect cell expression system (Bm5/Bm5.NPV.CAT) to establish the optimum temperature for both cell growth and virus infection. Bm5 cell growth was found to be limited at temperatures below 22°C and ceased completely at temperatures above 34°C. In the range between 24 and 28°C, final cell densities always reached 96% of the highest achievable viable cell density. The shortest population doubling time was obtained at 28°C. Overall, a consistent increase in metabolism with increasing temperatures was observed. During the infection/viral replication phase, an increase in the temperature from 25 to 31°C resulted in a faster decrease in viable cell density and an earlier production of chloramphenicol acetyltransferase (CAT). Furthermore, protein yield at temperatures above 28°C was significantly reduced. Overall, the best temperature for the infection phase for the Bm5/Bm5.NPV expression system was found to be 25°C when the cells are cultured in serum free media. 相似文献
92.
Various aspects on probabilistic modelling of cleavage fracture are discussed. The investigation involves consideration of
a unit cell with an explicitly modelled void. The results from this model are compared with results for the case when the
void content is accounted for in the sense of a Gurson-Tvergaard law. It is found that explicit modelling of the void can
give substantially different results for the fracture probability. The effect depends on the exponent in the assumed Weibull
distribution, the threshold stress, the constraint conditions and the hardening of the material.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
93.
采用气囊作内模进行浇筑桥面板混凝土 ,加快了预制桥面板施工进度 ,确保工程施工质量 ,并降低了工程施工成本 相似文献
94.
This paper addresses the problem of bandwidth allocation under the weighted maximum rate constrained link sharing policy and proves a key theory in the condition of allocation termination. We propose several algorithms with various worst-case and average-case time complexities, and evaluate their computation elapse times. 相似文献
95.
Intelligent scheduling with tabu search: An application to jobs with linear delay penalties and sequence-dependent setup costs and times 总被引:3,自引:0,他引:3
In this article we study thetabu search (TS) method in an application for solving an important class of scheduling problems. Tabu search is characterized by integrating artificial intelligence and optimization principles, with particular emphasis on exploiting flexible memory structures, to yield a highly effective solution procedure. We first discuss the problem of minimizing the sum of the setup costs and linear delay penalties when N jobs, arriving at time zero, are to be scheduled for sequential processing on a continuously available machine. A prototype TS method is developed for this problem using the common approach of exchanging the position of two jobs to transform one schedule into another. A more powerful method is then developed that employs insert moves in combination with swap moves to search the solution space. This method and the best parameters found for it during the preliminary experimentation with the prototype procedure are used to obtain solutions to a more complex problem that considers setup times in addition to setup costs. In this case, our procedure succeeded in finding optimal solutions to all problems for which these solutions are known and a better solution to a larger problem for which optimizing procedures exceeded a specified time limit (branch and bound) or reached a memory overflow (branch and bound/dynamic programming) before normal termination. These experiments confirm not only the effectiveness but also the robustness of the TS method, in terms of the solution quality obtained with a common set of parameter choices for two related but different problems. 相似文献
96.
A. W. MacGregor 《Journal of the Institute of Brewing》1996,102(2):97-102
Molecular technologies have been developed for the transformation of barley. These technologies complement current methods of barley breeding. In addition, they offer the potential of altering specific components in barley that affect malting quality and of introducing foreign genes, controlling desirable traits, into barley. Application of genetic engineering to improving malt quality factors such as cell wall degradation, protein modification, starch hydrolysis and flavour stability, is discussed. Limitations to the use of this technology for improving malt-related functional properties of barley components such as cell walls and starch granules are also evaluated. Some possible constraints to the utilization of genetic engineering for malt quality improvements are identified. 相似文献
97.
本文探讨了TEM室测量电话通信设备辐射干扰的原理、方法,得出了在线电话终端0.15~30MHz频段辐射干扰测量结果,并进行了分析。 相似文献
98.
Joonyoung Park Sung-Hwan Han S. Senthilarasu Soo-Hyoung Lee 《Solar Energy Materials & Solar Cells》2007,91(8):751-753
Recent studies have reported that a thin interlayer between poly(3, 4-ethylene dioxythiophene)-poly(styrene sulfonic acid) (PEDOT: PSS) and an emissive polymer layer leads to a large increase in the performances of polymer light-emitting diodes (PLEDs) by preventing significant quenching of the radiative excitons at the PEDOT: PSS interface; therefore, acting as an efficient exciton-blocking layer. Using the similar idea, a thin interlayer was fabricated between PEDOT: PSS and the active layer of conjugated polymers/methanofullerene composites in a plastic solar cell. The interlayer consisted of a poly(fluorene)-based hole transporter spin-coated directly on top of the PEDOT: PSS layer. The devices with the interlayer exhibited a higher efficiency than in those without the interlayer. 相似文献
99.
David C. Whitehead Katherine M. Goulden Roy D. Hartley 《Journal of the science of food and agriculture》1985,36(5):311-318
The distribution of 10 nutrient elements was assessed in three fractions of herbage samples of perennial ryegrass, tall fescue, white clover and lucerne. The cell wall fraction, prepared by mechanical disintegration of undried material, contained the following proportions of the total herbage content of the major elements: N, 2.9–16.4%; P, 3.0–6.9%; S, 3.6–17.2%; Ca, 11.3–51.8%; Mg. 6.4–27.6% and K, 0.1–1.7%. Substantial though more variable proportions of the trace element cations, Fe, Mn, Zn and Cu were also present in the cell wall fraction. The extent to which the elements were soluble in water was assessed by analysis of the water-insoluble fraction prepared from freeze-dried samples of each herbage. The proportions of the total herbage content of the major elements soluble in water were: N, 30–57%; P, 80–92%; S, 35–71%; Ca, 48–69%; Mg. 65–83% and K, 90–96%. With the trace element cations the proportions soluble in water were more variable, though they were generally less than 70% of the total. The extent to which the various elements were soluble in 80% aqueous ethanol was assessed by analysis of the insoluble fraction prepared from freeze-dried materials. With each of the major elements, smaller proportions of the total herbage content were soluble in 80% ethanol than in water, which may reflect in part the lower solubility of proteins and pectin. With the trace element cations, the differences in solubility between 80% ethanol and water were generally small and inconsistent. 相似文献
100.
CDC15, an essential cell cycle gene in Saccharomyces cerevisiae, encodes a protein kinase domain 总被引:17,自引:0,他引:17
The cell division cycle gene CDC15 is essential for the late nuclear division in the yeast Saccharomyces cerevisiae. The amino acid sequence of the 974 amino acids/110 kDa CDC15 gene product, as deduced from the nucleotide sequence, includes an aminoterminal protein kinase domain which contains a primary sequence mosaic showing patterns specific for protein serine/threonine kinases besides those for protein tyrosine kinases. Many protein kinases non-essential for growth are known. CDC15 represents an essential protein kinase like CDC7 and CDC28. A carboxyterminal deletion of 32 amino acids renders the protein inactive. 相似文献